Licor
Odyssey Clx
9140-09
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The Odyssey Clx is used for western blot imaging. The protocol is very similar to conventional western blotting. It calls specifically for Millipore PVDF membranes, and Odyssey blocking solution used for blocking, primary and secondary incubations. You can use any primary antibody, however, the secondary antibody is specific to Licor, it is conjugated to a fluorophore in order to image. The Odyssey images based on near-IR fluorescence. The Odyssey can image western blot (NIR), in-cell western, gel shift, and tissue sections.
Western Blotting
Rat hepatoma whole cell lysates
Whole cell lysates were separated via SDS-PAGE, and transferred to a PVDF membrane (Millipore). Membrane was blocked in 1:3 OB:TBS for 1 hour at RT. Membrane was probed for b-actin at a 1:1000 dilution in 1:3 OB:TBST (0.1%tween20) overnight at 4 degrees C. Membrane was washed in and incubated with secondary antibody, IRDye680 goat-anti-rabbit 1:15,000. in 1:3 OB:TBST (0.1%tween20) with 0.02% SDS for 1 hour at RT.Membrane was imaged using auto intensity.
Load very small amount of protein marker, I use Licor chameleon at 1uL.
Nice crisp results. Imaging on this computer are quick and easy. Can image the same blot forever after you dry it out.
Can multiplex antibodies! Saves tons of time
N/A
EASY, saves time, user friendly
Tough to strip membranes
Would rather do this any day than chemi!!!
Product Name: Odyssey® CLx Molecular Imaging WorkstationSupplier Page