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Author Profile
Raul Peña
Lab manager
Fundación IMIM
Articles by Raul Peña
V5 Tag Detection
March 19, 2014
Protein tagging is a very usual method to detect proteins with no available good commercial antibodies. V5 epitope tag is derived ...
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Ubiquitination in Nuclei by FBXL5
March 14, 2014
SNA1 is the main effectors of EMT due to its gene repression activity. Stabilization and subcellular localization of the protein ...
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Cell Cycle Analysis. CDK1 Detection by WB
March 13, 2014
We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell ...
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MPM-2 - Cell Cycle Analysis by IF
February 17, 2014
MPM-2 antigens are strongly expressed within mitotic cells. In eukaryotes, MPM-2 phosphoproteins have been found in the ...
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E-Cadh as Epithelial Marker
January 28, 2014
The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. Cadherins are a group of ...
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Loading Control for WB: Tubulin
January 27, 2014
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families,...
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Alpha Amylase Detection in Pancreas
January 24, 2014
a-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and ...
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PTEN Detection by WB
January 23, 2014
PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers)...
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Akt Pathway Activation Analysis
January 22, 2014
Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has ...
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Akt Detection in MDCK Cells
January 21, 2014
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is ...
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GFP Detection by WB
January 20, 2014
GFP is a fluorescent protein that is used as protein tag in vitro. Its detection by WB can be used to normalize transfection or ...
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Cell Cycle and CDC25A
January 17, 2014
The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among ...
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Cell Adhesion and E-Cadherin Antibody Review
January 16, 2014
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in ...
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Glucagon Production in Mouse Pancreas
December 26, 2013
Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and...
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Insulin Detection in Pancreas
December 25, 2013
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism...
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Excellent Fixation with Consistent Cell Preservation
Arianna Napoli
A high-quality ready-to-use fixative that consistently preserves cellular morphology and marker expression. It simplifies workflows while providing reproducible...
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