SIGMA
Alpha Amylase antibody
A8273
α-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose.Pancreatic α-amylase randomly cleaves the α(1-4) glycosidic linkages of amylose to yield dextrin, maltose, or maltotriose. It adopts a double displacement mechanism with retention of anomeric configuration.The test for amylase is easier to perform than that for lipase, making it the primary test used to detect and monitor pancreatitis. Medical laboratories will usually measure either pancreatic amylase or total amylase. If only pancreatic amylase is measured, an increase will not be noted with mumps or other salivary gland trauma.
Immunofluorescence
Mice pancreas
1/200
1%FBS 1%BSA in TBS-T
Leyca Powervision
Not needed
DAB
Nice detection in paraffin samples
Heat mediated retrieval needed (citrate pH6).
Specific signal. strong signal
Any remarkable.
Strong signal in pancreatic cells. Great antibody