Cell Signalling
P-Akt Antibody
9275
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Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major focus of attention because of its critical regulatory role in diverse cellular processes, including cancer progression and insulin metabolism. These findings make Akt/PKB an important therapeutic target for the treatment of cancer, diabetes, laminopathies, stroke, and neurodegenerative disease.
Western Blot
MDCK cells
1/1000
Milk 5% in TBS-T
Anti Rb HRP
Not needed
ECL
Unique band at expected MW. P-thr 308 indicates active Akt pathway in response to DNA damage by gamma-Irradiation.
None
Specificity. Non-phosphorylated protein is not detected.
Any remarkable.
After Irradiation, cells undergoes in a phosphorylation of Akt protein, without an increase of total protein amount.