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Pavan Kumar Chodisetti
Postdoctoral Researcher
The Scripps Research Institute
Articles by Pavan Kumar Chodisetti
Convenient DNA Gel Extraction Kit From Qiagen
August 16, 2023
This kit is very useful for extraction of DNA from agarose gels especially if you have non-specific PCR-amplified DNA bands.
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Streamlined Plasmid DNA Purification Kit From Zymo Research
August 14, 2023
I do a lot of clonings in my lab as a part of making gene knockouts in bacteria. In this process, I have to make a lot of plasmid ...
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Reliable E. coli Competent Cells From New England BioLabs
August 14, 2023
I have constructed a relatively large shuttle vector that can be propagated in both Gram-positive (E. faecium) and Gram-negative ...
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Reliable Kit From ThermoFisher for Bacterial Live-Dead Cell Staining
August 14, 2023
I made a cell wall hydrolase knockout in E. coli and I observed decreased growth in a growth curve. To investigate whether the ...
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Fantastic enzyme to solubilize cell walls of bacteria
August 14, 2023
I work extensively with cell wall hydrolases (enzymes) of bacteria. To check the enzymatic activity, I have to solubilize the ...
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Tritiated-Meso-Diaminopimelic Acid From Moravek, a Proxy to Determine Peptidoglycan Incorporation in Bacteria
August 14, 2023
I have used 3H-mDAP as a proxy, to determine the amount of cell wall incorporation in a cell wall hydrolase mutant.
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Test strips to determine Minimum Inhibitory Concentration (MIC) of bacteria
August 14, 2023
I want to investigate the genetic connection between cell wall hydrolase knockouts of bacteria and cell wall-acting antibiotic ...
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Highly Recommended Genomic DNA Isolation Kit From New England BioLabs
August 14, 2023
In order to clone bacteria genes, I must extract genomic DNA (gDNA) using this kit, which is more efficient than the traditional ...
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Stain-Free Precast Gels From BIO-RAD for Polyacrylamide Gel Electrophoresis
August 05, 2023
I use these gels in my research to a great extent because of the short running time, and because they are readily available (you ...
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Detection of LAP+ Treg Cells by Flow Cytometry
Mitesh Dwivedi
The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans.
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