ThermoFisher Scientific
Phusion Hot Start II High-Fidelity DNA Polymerase
F549L
Info:Supplier Page
Phusion High-Fidelity DNA Polymerase was purchased to use in PCR in order to get 2kb DNA product with high fidelity. The results demonstrated utilizing the correct annealing time consistently yielded the target 2 kb product with high fidelity and a complete absence of sequence mutations.
PCR and cloning
genomic DNA from cell samples
set up an accurate annealing time
Phusion High-Fidelity DNA Polymerase was employed in the PCR amplification strategy to isolate a 2 kb target DNA product while minimizing replication errors. Optimization of the reaction conditions revealed that maintaining an accurate annealing time was essential to successfully and reliably obtain the desired high-fidelity product without introducing any detectable mutations.
PCR product with high fidelity
Product yields is relatively low
This DNA Polymerase could have good PCR product with high fidelity