DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(281) Fucoxanthin attenuates LPS-induced acute lung injury via inhibition of the TLR4/MyD88 signaling axisAgingDecember 11, 2020Li X, Huang R, Liu K, Li M, Luo H, Cui L, Huang L, Luo L.RT. After washed with PBS three times, cells were stained with DAPI at 1:1000 (Cell Signaling Technology, 4083) for 10 min, washed three times with PBS, and then subjected to image acquisition by Cytation 5 High content screening

(282) A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleusNature communicationsDecember 1, 2020Simion V, Zhou H, Haemmig S, Pierce JB, Mendes S, Tesmenitsky Y, Pérez-Cremades D, Lee JF, Chen AF, Ronda N, et al.temperature. Nuclei were stained for 5 min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5 µg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen, P36930). Images

(283) Inhibition of carnitine palmitoyl transferase 1A-induced fatty acid oxidation suppresses cell progression in gastric cancerArchives of Biochemistry and BiophysicsDecember 1, 2020Liqiang Wang, Changfeng Li, Yumei Song, ZhenKun Yanwere serum-starved for 1 h, stained for 10 min with BODIPY® 493/503 (D3922, Invitrogen) and DAPI (#4083, CST), and imaged by using an OLYMPUS BX53 microscope. Statistical procedures Statistical analysis

(284) HER2-positive breast cancer that resists therapeutic drugs and ionizing radiation releases sphingomyelin-based molecules to circulating blood serumMolecular and clinical oncologyDecember 1, 2020Monzen S, Tatara Y, Mariya Y, Chiba M, Wojcik A, Lundholm L.Cell Signaling Technology) were used as secondary antibodies. The cell nuclear staining for fluorescence analysis DAPI (no. 4083; Cell Signaling Technology) was used. Fluorescence signals were detected using a fluorescence microscope

(285) lncRNA KTN1‑AS1 promotes glioma cell proliferation and invasion by negatively regulating miR‑505‑3pOncology reportsDecember 1, 2020Mu Y, Tang Q, Feng H, Zhu L, Wang Y.ProteinTech Group, Inc.)] for 1 h at room temperature. Nuclei were stained with DAPI (1 µg/ml; cat. no. 4083s; Cell Signaling Technology, Inc.) for 10 min. Cell slides were subsequently collected and placed on

(286) A versatile toolkit for CRISPR-Cas13-based RNA manipulation in DrosophilaGenome biologyNovember 17, 2020Huynh N, Depner N, Larson R, King-Jones K.dilution buffer for 1 h at RT, and then washed in 1× PBS3T with either 1:50,000 DAPI (Cell Signaling #4083) or 1:2000 Nuclear Green DCS1 (Abcam ab138905) for 3 × 10 min. Samples were mounted in Vectashield mounting

(287) Fibroblast growth factor-2 alleviates the capillary leakage and inflammation in sepsisMolecular medicine (Cambridge, Mass.)November 13, 2020Pan X, Xu S, Zhou Z, Wang F, Mao L, Li H, Wu C, Wang J, Huang Y, Li D, et al.anti-p-P65 (9936), anti-P38 (8690), anti-p-P38 (9216), anti-IL-1β (12242), anti-TNF-α (3707) and DAPI (4083) were acquired from Cell Signaling Technology, USA. Anti-VE-cadherin (ab33168), anti-rabbit IgG H&L

(288) Histone H3K9 and H3K14 acetylation at the promoter of the LGALS9 gene is associated with mRNA levels in cervical cancer cellsFEBS Open BioNovember 1, 2020Erick Armenta‐Castro et al.Abcam) diluted 1 : 1000 in 1% BSA in PBS. Nuclei staining was performed with 0.1 µg·mL−1 DAPI Stain (4083; Cell Signaling Technology, Danvers, MA, USA). An isotype IgG control was applied as a negative control

(289) Metabolic Imaging Detects Resistance to PI3Kα Inhibition Mediated by Persistent FOXM1 Expression in ER⁺ Breast CancerCancer cellOctober 12, 2020Ros S, Wright AJ, D'Santos P, Hu DE, Hesketh RL, Lubling Y, Georgopoulou D, Lerda G, Couturier DL, Razavi P, et al.before a brief wash in 2X SSC plus 0.05% Tween 20. Slides were incubated with DAPI counterstain (Cat # 4083S, Cell Signaling Technologies), diluted to 10 μg/ml, for 5 min at room temperature in the dark. Slides

(290) Comparative Transcriptome Analysis of the Regenerating Zebrafish Telencephalon Unravels a Resource With Key Pathways During Two Early Stages and Activation of Wnt/β-Catenin Signaling at the Early Wound Healing StageFrontiers in Cell and Developmental BiologyOctober 9, 2020Yeliz Demirci, Gokhan Cucun, Yusuf Kaan Poyraz, Suhaib Mohammed, Guillaume Heger, Irene Papatheodorou, Gunes OzhanJackson Immunoresearch Laboratories). Nuclear staining was performed with 40 ,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology). MATERIALS AND METHODS Stab Wound Assay A transgenic reporter of Tcf/Lef-mediated

(291) Methods for histological characterization of cryo-induced myocardial infarction in a rat modelActa HistochemicaOctober 1, 2020Matthew Alonzo, Monica Delgado, Carol Cleetus, Shweta Anil Anil Kumar, Vikram Thakur, Munmun Chattopadhyay, Binata Joddarwith 1X PBS for 3 times, stained with DAPI (Cell Signaling Technology Inc., Danvers, MA, USA Cat. # 4083S; 20 mg/ml stock solution). DAPI was diluted at 1:50,000 in deionized water and incubated for 2–3 minutes.

(292) DDX3X Suppresses the Susceptibility of Hindbrain Lineages to MedulloblastomaDevelopmental cellAugust 24, 2020Patmore DM, Jassim A, Nathan E, Gilbertson RJ, Tahan D, Hoffmann N, Tong Y, Smith KS, Kanneganti TD, Suzuki H, et al.Invitrogen, A-21206, A-21207 or A10043). Sections were then counterstained using DAPI (Cell Signaling, 4083) and mounted using ProLong Gold antifade mountant (Thermo Fisher Scientific, P36930). Digital images

(293) Long non‑coding RNA FLVCR1‑AS1 promotes glioma cell proliferation and invasion by negatively regulating miR‑30b‑3pMolecular medicine reportsAugust 1, 2020Gao W, Li H, Liu Y, Zhang Y, Zhao H, Liu F.ProteinTech Group, Inc.] at room temperature for 1 h. Cell nuclei were stained with DAPI (1 µg/ml; cat. no. 4083s; Cell Signaling Technology, Inc.) for 15 min and images were captured using a fluorescence microscope

(294) A novel BCMA PBD-ADC with ATM/ATR/WEE1 inhibitors or bortezomib induce synergistic lethality in multiple myelomaLeukemiaAugust 1, 2020Lijie Xing et al.Medical) and DNA damage by γH2AX (CST-9718, Cell Signaling Technology), with counterstaining by DAPI (CST-4083, Cell Signaling Technology) to locate nuclei. Immunohistochemical images were taken on Zeiss Inverted

(295) Long non-coding RNA EWSAT1 contributes to the proliferation and invasion of glioma by sponging miR-152-3pOncology lettersAugust 1, 2020Yang H, Chen W, Jiang G, Yang J, Wang W, Li H.ProteinTech Group, Inc.)] at room temperature for 1 h. Cell nuclei were then incubated with DAPI at 4°C (1 µg/ml; cat. no. 4083s; Cell Signaling Technology, Inc.) for 15 min and observed under a fluorescence microscope (Nikon Corporation)

(296) Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathwaysStem cell research & therapyJuly 16, 2020Liu F, Fang Y, Hou X, Yan Y, Xiao H, Zuo D, Wen J, Wang L, Zhou Z, Dang X, et al.anti-mouse IgG (A-11005, Invitrogen, USA). The cells were then counterstained using 0.5 μg/ml of DAPI (4083, Cell Signaling Technology, USA) for 15 min at room temperature. After rinsing with PBS, the chambers

(297) Phospho-Tyr705 of STAT3 is a therapeutic target for sepsis through regulating inflammation and coagulationCell communication and signaling : CCSJuly 8, 2020Xu S, Pan X, Mao L, Pan H, Xu W, Hu Y, Yu X, Chen Z, Qian S, Ye Y, et al.#ab6717), anti-mouse PE (Abcam, #ab130774), anti-rabbit Alexa Fluor (Abcam, #ab150078) and DAPI (CST, #4083). The following ELISA kits were used: TNF-α (Multi Sciences, #EK282), IL-1β (Abcam, #197742), IL-6 (Multi

(298) IL-17 aggravates renal injury by promoting podocyte injury in children with primary nephrotic syndromeExperimental and therapeutic medicineJuly 1, 2020Zhai S, Sun B, Zhang Y, Zhao L, Zhang L.the slides were incubated in a wet box at 20‑37˚C for 1 h. A concentration of 5 µg/ml DAPI (cat. no. 4083; Cell Signaling Technology, Inc.) was subsequently added before the slides were incubated in the dark

(299) CD47 is a negative regulator of intestinal epithelial cell self-renewal following DSS-induced experimental colitisScientific reportsJune 23, 2020He Y, Sun X, Rong W, Yang R, Liang H, Qi Y, Li L, Zen K.The following antibodies were used: anti-c-Myc (Cell Signaling, 2840 s), anti-KLF4 (Cell Signaling, 4083 s), anti-Oct-4A Scientific Reports | (2020) 10:10180 | https://doi.org/10.1038/s41598-020-67152-w 2 www.nature.com/scientificreports/ www.nature.com/scientificreports Figure

(300) Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolismNature metabolismJune 1, 2020Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, Rahman M, Brown KR, Ross C, Usaj M, et al.antibodies (1:500, Invitrogen A-21245) and counterstained with 1 μg/ml DAPI (Cell Signaling Technology, 4083S) for 45 minutes in the dark. Cells were visualized by confocal microscopy (Zeiss LSM 880). RNA-sequencing Sample

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