DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(241) Microengineered Multi-Organoid System from hiPSCs to Recapitulate Human Liver-Islet Axis in Normal and Type 2 DiabetesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2022Tao T, Deng P, Wang Y, Zhang X, Guo Y, Chen W, Qin J.secondary antibodies (1:500, CST) at room temperature for 1 h. Nuclei were stained with DAPI (1:4000, CST 4083). Real‐Time Quantitative PCR To quantify the gene expression levels in liver and islet organoids,

(242) Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosisNatureFebruary 1, 2022Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, Min R, Wu Z, Li W, Miao R, et al.ImmunoResearch). Nuclei were counterstained with DAPI (4,6-diamidino-2-phenylindole) (Cell Signaling Technology, #4083). Slides were mounted using Aqua-Poly/Mount (Dako). Images were captured using a laser scanning confocal

(243) The Effects of Mechanical Loading Variations on the Hypertrophic, Anti-Apoptotic, and Anti-Inflammatory Responses of Differentiated Cardiomyocyte-like H9C2 CellsCellsJanuary 29, 2022Zevolis E, Philippou A, Moustogiannis A, Chatzigeorgiou A, Koutsilieris M.conjugated with CF 488A (1:2000, Biotium, Fremont CA, USA), and after staining also with DAPI (1 µg/mL; 4083; Cell Signaling), they were viewed under a microscope (Olympus BX40; Olympus Corporation, Tokyo, Japan).

(244) Mechanical force promotes dimethylarginine dimethylaminohydrolase 1-mediated hydrolysis of the metabolite asymmetric dimethylarginine to enhance bone formationNature communicationsJanuary 10, 2022Xie Z, Hou L, Shen S, Wu Y, Wang J, Jie Z, Zhao X, Li X, Zhang X, Chen J, et al.(1:1000; Molecular Probes, A21206) were used as secondary antibodies. DAPI (Cell Signaling Technology, #4083) and DyLight™ 594 Phalloidin (Cell Signaling Technology, #12877) were used for counterstaining. All

(245) PDIA2 Bridges Endoplasmic Reticulum Stress and Metabolic Reprogramming During Malignant Transformation of Chronic ColitisFrontiers in oncologyJanuary 1, 2022Tao J, Yin L, Wu A, Zhang J, Zhang J, Shi H, Liu S, Niu L, Xu L, Feng Y, et al.anti-rabbit IgG from Vector Laboratories (Burlingame, CA, USA), DAPI from Cell Signaling Technology (CST) (4083S, USA), and Annexin-FITC/PI from Shanghai Yisheng Biotechnology Co., Ltd (Shanghai, China). D (+)-glucose

(246) Primary Tumor Suppression and Systemic Immune Activation of Macrophages through the Sting Pathway in Metastatic Skin TumorYonsei medical journalJanuary 1, 2022Synn CB, Kim DK, Kim JH, Byeon Y, Kim YS, Yun MR, Lee JM, Lee W, Lee EJ, Lee S, et al.(PerkinElmer) according to the manufacturer’s protocol. The cell nuclei were stained with DAPI (cat no. #4083). The pIRF3 (cat no. #3033) and NF-κB p65 (cat no. #8242) antibodies were purchased from Cell Signaling Technology

(247) Three-dimensional analyses of vascular network morphology in a murine lymph node by X-ray phase-contrast tomography with a 2D Talbot arrayFrontiers in immunologyJanuary 1, 2022Schwarzenberg FL, Schütz P, Hammel JU, Riedel M, Bartl J, Bordbari S, Frank SC, Walkenfort B, Busse M, Herzen J, et al.10 min in 0.1% PBT and counterstained with DAPI as a nuclear counterstain (Cell Signaling, Cat.Nr.: 4083S, Cambridge, UK) 1:5000 in 0.1% PBT for 30 min. In slides where unconjugated antibodies were used, secondary

(248) miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1Oxidative medicine and cellular longevityJanuary 1, 2022Bian C, Luan Z, Zhang H, Zhang R, Gao J, Wang Y, Li J, Ren H.antibody,"Mouse antibody isotype control, #5415, for IP, Cell Signaling Technology" DAPI,"For nucleus, #4083, for IF, Cell Signaling Technology" Anti-rabbit IgG,"Anti-rabbit, #3678, for WB, Cell Signaling Technology" Anti-mouse

(249) A spatiotemporal steroidogenic regulatory network in human fetal adrenal glands and gonadsFrontiers in endocrinologyJanuary 1, 2022Wang Y, Guo B, Guo Y, Qi N, Lv Y, Ye Y, Huang Y, Long X, Chen H, Su C, et al.The slides were washed three times in PBS for 5 min each, and the nuclei were then stained with DAPI (4083S, CST). Fluorescence images were captured using a laser scanning confocal microscope (TCS SP8, Laika

(250) Spontaneous formation of neutrophil extracellular traps is associated with autophagyScientific reportsDecember 14, 2021Guo Y, Gao F, Wang X, Pan Z, Wang Q, Xu S, Pan S, Li L, Zhao D, Qian J.min and exposed to ProLong Gold Antifade Reagent (Cell Signaling Technology, Beverly, USA, catalog # 4083S and catalog # 9071S, respectively). Cells were visualized using a confocal TCS SP8 microscope (Leica Microsystems

(251) Meiotic Cas9 expression mediates gene conversion in the male and female mouse germlinePLoS biologyDecember 1, 2021Weitzel AJ, Grunwald HA, Weber C, Levina R, Gantz VM, Hedrick SM, Bier E, Cooper KL.anti-rabbit, 1:250 each] in block with 300 nM DAPI [Cell Signaling Technology (MA, USA), catalog # 4083S] and incubated at 4°C for 18 hours. We then washed 3× for 30′ in PBST and mounted under coverslips in

(252) Bioengineered miR-124-3p prodrug selectively alters the proteome of human carcinoma cells to control multiple cellular components and lung metastasis in vivoActa pharmaceutica Sinica. BDecember 1, 2021Deng L, Petrek H, Tu MJ, Batra N, Yu AX, Yu AM.anti-rabbit Alexa Fluor® 488-conjugated secondary antibody and then 4ʹ,6-diamidino-2-phenylindole (DAPI; #4083, Cell signaling Technology) for staining nuclei or Alexa Fluor® 594 Phalloidin (#12877, Cell signaling Technology

(253) Haem relieves hyperoxia-mediated inhibition of HMEC-1 cell proliferation, migration and angiogenesis by inhibiting BACH1 expressionBMC OphthalmologyDecember 1, 2021Lan Jian, Yang Mei, Chen Xing, Yuan Rongdito the instructions. Finally, the cells were washed with PBS for 5 min, stained with DAPI (1:1000, 4083, CST, USA) for 5 min, washed with PBS and sealed with an anti-fluorescence quenching solution [18].

(254) p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal DifferentiationInternational journal of stem cellsNovember 30, 2021Liu Y, Zhang P, Huang W, Liu F, Long D, Peng W, Dang X, Zeng X, Zhou R.anti-mouse IgG (A-11005, Invitrogen, USA). The slides were then counterstained using 0.5 μg/ml of DAPI (#4083, Cell Signaling Technology, USA) for 15 min at room temperature. After rinsing with PBS, the chambers

(255) hPMSCs-Derived Exosomal miRNA-21 Protects Against Aging-Related Oxidative Damage of CD4+ T Cells by Targeting the PTEN/PI3K-Nrf2 AxisFrontiers in ImmunologyNovember 23, 2021Yanlian Xiong et al.dysfunction, thereby clinically alleviating immunosenescence. Materials And Methods Reagents DAPI(#4083) and antibodies against Histon H3 (#4499), Nrf2 (#12721), PI3K (#4249), Akt (#9272), β-actin (#4970),

(256) Schisandrin A protects against isoproterenol‑induced chronic heart failure via miR‑155Molecular Medicine ReportsNovember 22, 2021Lijing Gao, Ting Li, Shufen Li, Zhuohui Song, Yongli Chang, Li Yuanadded and incubated at 37°C for 1 h. The subsequent steps were performed in the dark. DAPI (cat. no. 4083; Cell Signaling Technology, Inc.) was used to incubate at room temperature for 5 min for nuclear counterstaining.

(257) Pharmacological inhibition of BACE1 suppresses glioblastoma growth by stimulating macrophage phagocytosis of tumor cellsNature cancerNovember 1, 2021Zhai K, Huang Z, Huang Q, Tao W, Fang X, Zhang A, Li X, Stark GR, Hamilton TA, Bao S.Supplementary Table 1) for one hour at room temperature, counterstained with DAPI (Cell Signaling, 4083, 1:5000) and sealed with mounting medium (Sigma-Aldrich, F4680). Finally, images were captured by a

(258) Homogeneity or heterogeneity, the paradox of neurovascular pericytes in the brainGliaOctober 1, 2021Zhang H, Zhang X, Hong X, Tong X.sections were incubated with 4′,6′‐diamidino‐2‐phenylindole dihydrochloride (DAPI, 1:1500, Cell Signaling 4083S) for 15 min. The primary antibodies included goat antibody to Pdgfrβ (1:500; R&D system AF1042), GFAP

(259) Interpretation of cancer mutations using a multi-scale map of protein systemsScienceOctober 1, 2021Fan Zheng et al.Goat anti-Mouse (1:2000 dilution, A11003, Life Technologies, Eugene, OR, USA). DAPI (1:1000 dilution, 4083, Cell Signaling, Danvers, MA, USA) was utilized to counterstain the nuclei. Fluorescent images were

(260) NG2 glia-derived GABA release tunes inhibitory synapses and contributes to stress-induced anxietyNature communicationsSeptember 30, 2021Zhang X, Liu Y, Hong X, Li X, Meshul CK, Moore C, Yang Y, Han Y, Li WG, Qi X, et al.Alexa Fluor 647 (1:500, Invitrogen A21450). Sections were incubated with DAPI (1:1000, Cell Signaling 4083 S) for 15 min and mounted on glass slides in Fluoromount™ Aqueous Mounting Medium (AQUA-MOUNT, REF 13800).

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