DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(241) Ganoderma Lucidum Triterpenoids Improve Maternal Separation-Induced Anxiety- and Depression-like Behaviors in Mice by Mitigating Inflammation in the Periphery and BrainNutrientsMay 28, 2022Xue Mi, Gui-Rong Zeng, Jie-Qing Liu, Zhou-Song Luo, Ling Zhang, Xiao-Man Dai, Wen-Ting Fang, Jing Zhang, Xiao-Chun Chenat 4 °C overnight and at room temperature for 1 h. The brain sections were exposed to DAPI (1 μg/mL; 4083, CST, Danvers, MA, USA) for nuclear staining for 5 min, and then cover slips were mounted on slides

(242) Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s DiseaseFrontiers in PharmacologyMay 25, 2022Zhao Dai et al.37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for western blot. 2.5 Network Visualization and Statistical Analysis Statistical

(243) Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractographyeLifeMay 20, 2022Yan M, Yu W, Lv Q, Lv Q, Bo T, Chen X, Liu Y, Zhan Y, Yan S, Shen X, et al.Alexa Fluor-conjugated secondary antibodies at room temperature for 3 hr, and DAPI (Cell signaling Cat# 4083 s) for 30 min at room temperature. The following primary antibodies were used: CaMKIIa (1:200, Abcam,

(244) Brain Extract of Subacute Traumatic Brain Injury Promotes the Neuronal Differentiation of Human Neural Stem Cells via AutophagyJournal of Clinical MedicineMay 11, 2022Zhenghui He et al.MA, USA) were used as secondary antibodies. Nuclei were counterstained with DAPI stain (1:10,000; #4083, Cell Signaling Technology, Danvers, MA, USA). The slides were viewed using the Zeiss Axio observer

(245) Treatment of SARS-CoV-2-induced pneumonia with NAD(+) and NMN in two mouse modelsCell discoveryApril 29, 2022Jiang Y, Deng Y, Pang H, Ma T, Ye Q, Chen Q, Chen H, Hu Z, Qin CF, Xu Z.human IgG (1:2000; Invitrogen). Nuclei were counterstained with DAPI (Cell Signaling Technology, Cat# 4083s). Fluorescent images were captured using a confocal laser-scanning microscope (Carl Zeiss, LSM700 and

(246) Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microgliaJournal of neuroinflammationApril 12, 2022Xiang X, Tang X, Yu Y, Xie S, Liu L, Chen M, Zhang R, Kang X, Zheng Y, Yang G, et al.1:400) for 1 h at room temperature. Sections were then washed in PBS, incubated with DAPI (1:1000, CST, #4083) in PBS for 10 min. Subsequently, sections were washed in PBS buffer, mounted onto microscope slides

(247) Coupled small molecules target RNA interference and JAK/STAT signaling to reduce Zika virus infection in Aedes aegyptiPLOS PathogensApril 4, 2022Chasity E. Trammell, Gabriela Ramirez, Irma Sanchez-Vargas, Laura A. St Clair, Oshani C. Ratnayake, Shirley Luckhart, Rushika Perera, Alan G. Goodmanmembranes for 1 h at room temperature in the dark. Samples were stained with DAPI (1:100; Cell Signaling 4083), mounted onto coverslips using ProLong Diamond Antifade Mountant (Invitrogen P36961), and imaged using

(248) Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for ovarian endometriosisReproductive biology and endocrinology : RB&EMarch 29, 2022Murakami M, Osuka S, Muraoka A, Hayashi S, Bayasula, Kasahara Y, Sonehara R, Hariyama Y, Shinjo K, Tanaka H, et al.for 1 h at room temperature. Nuclear staining was carried out using 4',6-diamidino-2-phenylindole (4083, 1:1000, Cell Signaling Technology). Visualization was performed using a confocal laser-scanning microscope

(249) The Effect of Ex Vivo Human Serum from Liver Disease Patients on Cellular Protein Synthesis and GrowthCellsMarch 24, 2022Allen SL, Seabright AP, Quinlan JI, Dhaliwal A, Williams FR, Fine NHF, Hodson DJ, Armstrong MJ, Elsharkaway AM, Greig CA, et al.A32731, Thermo Fisher Scientific) as previously described [18]. Nuclei were stained with DAPI (1:5000, 4083, Cell Signaling Technology (CST), Leiden, The Netherlands). Image acquisition was performed using a

(250) Ferroptosis in oligodendrocyte progenitor cells mediates white matter injury after hemorrhagic strokeCell death & diseaseMarch 23, 2022Shen D, Wu W, Liu J, Lan T, Xiao Z, Gai K, Hu L, Luo Z, Wei C, Wang X, et al.secondary antibodies for 1 h. Nuclei were stained with 1 μg/ml 4’,6-diamidino-2-phenylindole (DAPI, 4083 S, CST) for 10 min. Images were acquired by fluorescence microscope (Nikon ECLIPSE Ci) or confocal microscope

(251) Somatostatin-evoked Aβ catabolism in the brain: Mechanistic involvement of α-endosulfine-K(ATP) channel pathwayMolecular psychiatryMarch 1, 2022Watamura N, Kakiya N, Nilsson P, Tsubuki S, Kamano N, Takahashi M, Hashimoto S, Sasaguri H, Saito T, Saido TC.tyramide-enhanced FITC or rhodamine for 10 min. Finally, sections were treated with DAPI (Cell Signaling Technology 4083 S) diluted in TNB buffer before mounting with PermaFluor (Thermo Fisher Scientific TA-030-FM). Sections

(252) Diverse Macrophages Constituted the Glioma Microenvironment and Influenced by PTEN StatusFrontiers in ImmunologyFebruary 21, 2022Fengqi Zhou et al.for 1 h at room temperature, counterstained with 4,6-dianmino-2-phenylindole (DAPI) (Cell Signaling, 4083, 1:5,000), and sealed with mounting medium (Sigma-Aldrich, F4680). Statistical Analysis The differences

(253) Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinomaCell death & diseaseFebruary 17, 2022Ling Z, Li W, Hu J, Li Y, Deng M, Zhang S, Ren X, Wu T, Xia J, Cheng B, et al.incubation in the dark at room temperature. The cells were then counterstained with 0.5 ug/mL of DAPI (#4083 s, CST, Danvers, MA, USA) for 5 min at room temperature and washed with PBS three times. The samples

(254) DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3JCI insightFebruary 8, 2022Borza CM, Bolas G, Bock F, Zhang X, Akabogu FC, Zhang MZ, de Caestecker M, Yang M, Yang H, Lee E, et al.antibody (1:1000, Abcam, ab225225) for 12 hours at 4°C, then incubated with DAPI (1:200, Cell Signaling, 4083) in PBS for 10 minutes at room temperature. Slides were mounted with Prolong Gold Antifade reagent (Invitrogen,

(255) Exogenous melatonin prevents type 1 diabetes mellitus-induced bone loss, probably by inhibiting senescenceOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USAFebruary 1, 2022Gong Z, Da W, Tian Y, Zhao R, Qiu S, Wu Q, Wen K, Shen L, Zhou R, Tao L, et al.at room temperature for 1 h. After washing with cold PBS 3 times, the cells were stained with DAPI (4083, 0.1 μg/ml, CST, America) for 5 min. After washing with cold PBS 3 times, images were captured with

(256) Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosisNatureFebruary 1, 2022Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, Min R, Wu Z, Li W, Miao R, et al.ImmunoResearch). Nuclei were counterstained with DAPI (4,6-diamidino-2-phenylindole) (Cell Signaling Technology, #4083). Slides were mounted using Aqua-Poly/Mount (Dako). Images were captured using a laser scanning confocal

(257) Microengineered Multi-Organoid System from hiPSCs to Recapitulate Human Liver-Islet Axis in Normal and Type 2 DiabetesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2022Tao T, Deng P, Wang Y, Zhang X, Guo Y, Chen W, Qin J.secondary antibodies (1:500, CST) at room temperature for 1 h. Nuclei were stained with DAPI (1:4000, CST 4083). Real‐Time Quantitative PCR To quantify the gene expression levels in liver and islet organoids,

(258) The Effects of Mechanical Loading Variations on the Hypertrophic, Anti-Apoptotic, and Anti-Inflammatory Responses of Differentiated Cardiomyocyte-like H9C2 CellsCellsJanuary 29, 2022Zevolis E, Philippou A, Moustogiannis A, Chatzigeorgiou A, Koutsilieris M.conjugated with CF 488A (1:2000, Biotium, Fremont CA, USA), and after staining also with DAPI (1 µg/mL; 4083; Cell Signaling), they were viewed under a microscope (Olympus BX40; Olympus Corporation, Tokyo, Japan).

(259) Mechanical force promotes dimethylarginine dimethylaminohydrolase 1-mediated hydrolysis of the metabolite asymmetric dimethylarginine to enhance bone formationNature communicationsJanuary 10, 2022Xie Z, Hou L, Shen S, Wu Y, Wang J, Jie Z, Zhao X, Li X, Zhang X, Chen J, et al.(1:1000; Molecular Probes, A21206) were used as secondary antibodies. DAPI (Cell Signaling Technology, #4083) and DyLight™ 594 Phalloidin (Cell Signaling Technology, #12877) were used for counterstaining. All

(260) Primary Tumor Suppression and Systemic Immune Activation of Macrophages through the Sting Pathway in Metastatic Skin TumorYonsei medical journalJanuary 1, 2022Synn CB, Kim DK, Kim JH, Byeon Y, Kim YS, Yun MR, Lee JM, Lee W, Lee EJ, Lee S, et al.(PerkinElmer) according to the manufacturer’s protocol. The cell nuclei were stained with DAPI (cat no. #4083). The pIRF3 (cat no. #3033) and NF-κB p65 (cat no. #8242) antibodies were purchased from Cell Signaling Technology

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DAPI

DAPI from Cell Signaling Technology

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DAPI from Cell Signaling Technology

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