DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(221) Quinolizidines as Novel SARS-CoV-2 Entry InhibitorsInternational Journal of Molecular SciencesAugust 25, 2022Li Huang, Lei Zhu, Hua Xie, Jeffery Shawn Goodwin, Tanu Rana, Lan Xie, Chin-Ho Chenwith Prolong® Gold Anti-Fade Reagent with DAPI (Cell Signaling Technology, Danvers, MA, USA, Cat. # 4083S). Confocal images were acquired using a Nikon TE2000-U laser-scanning confocal microscope (Nikon, Tokyo,

(222) Transplantation of insulin-producing cells derived from human mesenchymal stromal/stem cells into diabetic humanized miceStem cell research & therapyJuly 26, 2022Ghoneim MA, Gabr MM, Refaie AF, El-Halawani SM, Al-Issawi MM, Elbassiouny BL, Kader MAAE, Ismail AM, Zidan MF, Karras MS, et al.anti-rabbit IgG (H + L) (# 4413, Cell Signaling Technology). The nuclei were counterstained with DAPI (# 4083, Cell Signaling Technology). For intracellular staining for flow cytometry: Primary monoclonal antibodies

(223) Single-cell RNA sequencing of subcutaneous adipose tissues identifies therapeutic targets for cancer-associated lymphedemaCell discoveryJune 21, 2022Liu X, Yuan M, Xiang Q, Li Z, Xu F, Chen W, Chen J, Huang J, Yu N, Zhou Z, et al.Fluor 488 goat anti-rabbit (A-11008, Invitrogen) secondary antibody at room temperature for 1 h. DAPI (4083S, Cell Signaling Technology) was used for nuclear counterstaining. The cells were imaged using the Axio

(224) miR‐181b regulates vascular endothelial aging by modulating an MAP3K3 signaling pathwayThe FASEB JournalJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G. McCoy, Daniel Pérez‐Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtemperature. Nuclei were stained for 5min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5μg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen). Images were acquired

(225) A novel frameshift variant in the TSPAN12 gene causes autosomal dominant FEVRMolecular genetics & genomic medicineJune 1, 2022Peng L, Dai E, Xiao H, Zhao R, He Y, Li S, Yang M, Yang Z, Zhao P.Plus 488 secondary antibody (1:300 dilution; CAT# A32723; Invitrogen) and DAPI (1:500 dilution; CAT# 4083; Cell Signaling Technology). Images were captured on an LSM 800 confocal scanning microscope (Zeiss,). 3

(226) Ganoderma Lucidum Triterpenoids Improve Maternal Separation-Induced Anxiety- and Depression-like Behaviors in Mice by Mitigating Inflammation in the Periphery and BrainNutrientsMay 28, 2022Xue Mi, Gui-Rong Zeng, Jie-Qing Liu, Zhou-Song Luo, Ling Zhang, Xiao-Man Dai, Wen-Ting Fang, Jing Zhang, Xiao-Chun Chenat 4 °C overnight and at room temperature for 1 h. The brain sections were exposed to DAPI (1 μg/mL; 4083, CST, Danvers, MA, USA) for nuclear staining for 5 min, and then cover slips were mounted on slides

(227) Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s DiseaseFrontiers in PharmacologyMay 25, 2022Zhao Dai et al.37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for western blot. 2.5 Network Visualization and Statistical Analysis Statistical

(228) Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractographyeLifeMay 20, 2022Yan M, Yu W, Lv Q, Lv Q, Bo T, Chen X, Liu Y, Zhan Y, Yan S, Shen X, et al.Alexa Fluor-conjugated secondary antibodies at room temperature for 3 hr, and DAPI (Cell signaling Cat# 4083 s) for 30 min at room temperature. The following primary antibodies were used: CaMKIIa (1:200, Abcam,

(229) Brain Extract of Subacute Traumatic Brain Injury Promotes the Neuronal Differentiation of Human Neural Stem Cells via AutophagyJournal of Clinical MedicineMay 11, 2022Zhenghui He et al.MA, USA) were used as secondary antibodies. Nuclei were counterstained with DAPI stain (1:10,000; #4083, Cell Signaling Technology, Danvers, MA, USA). The slides were viewed using the Zeiss Axio observer

(230) Treatment of SARS-CoV-2-induced pneumonia with NAD(+) and NMN in two mouse modelsCell discoveryApril 29, 2022Jiang Y, Deng Y, Pang H, Ma T, Ye Q, Chen Q, Chen H, Hu Z, Qin CF, Xu Z.human IgG (1:2000; Invitrogen). Nuclei were counterstained with DAPI (Cell Signaling Technology, Cat# 4083s). Fluorescent images were captured using a confocal laser-scanning microscope (Carl Zeiss, LSM700 and

(231) Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microgliaJournal of neuroinflammationApril 12, 2022Xiang X, Tang X, Yu Y, Xie S, Liu L, Chen M, Zhang R, Kang X, Zheng Y, Yang G, et al.1:400) for 1 h at room temperature. Sections were then washed in PBS, incubated with DAPI (1:1000, CST, #4083) in PBS for 10 min. Subsequently, sections were washed in PBS buffer, mounted onto microscope slides

(232) Coupled small molecules target RNA interference and JAK/STAT signaling to reduce Zika virus infection in Aedes aegyptiPLOS PathogensApril 4, 2022Chasity E. Trammell, Gabriela Ramirez, Irma Sanchez-Vargas, Laura A. St Clair, Oshani C. Ratnayake, Shirley Luckhart, Rushika Perera, Alan G. Goodmanmembranes for 1 h at room temperature in the dark. Samples were stained with DAPI (1:100; Cell Signaling 4083), mounted onto coverslips using ProLong Diamond Antifade Mountant (Invitrogen P36961), and imaged using

(233) Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for ovarian endometriosisReproductive biology and endocrinology : RB&EMarch 29, 2022Murakami M, Osuka S, Muraoka A, Hayashi S, Bayasula, Kasahara Y, Sonehara R, Hariyama Y, Shinjo K, Tanaka H, et al.for 1 h at room temperature. Nuclear staining was carried out using 4',6-diamidino-2-phenylindole (4083, 1:1000, Cell Signaling Technology). Visualization was performed using a confocal laser-scanning microscope

(234) The Effect of Ex Vivo Human Serum from Liver Disease Patients on Cellular Protein Synthesis and GrowthCellsMarch 24, 2022Allen SL, Seabright AP, Quinlan JI, Dhaliwal A, Williams FR, Fine NHF, Hodson DJ, Armstrong MJ, Elsharkaway AM, Greig CA, et al.A32731, Thermo Fisher Scientific) as previously described [18]. Nuclei were stained with DAPI (1:5000, 4083, Cell Signaling Technology (CST), Leiden, The Netherlands). Image acquisition was performed using a

(235) Ferroptosis in oligodendrocyte progenitor cells mediates white matter injury after hemorrhagic strokeCell death & diseaseMarch 23, 2022Shen D, Wu W, Liu J, Lan T, Xiao Z, Gai K, Hu L, Luo Z, Wei C, Wang X, et al.secondary antibodies for 1 h. Nuclei were stained with 1 μg/ml 4’,6-diamidino-2-phenylindole (DAPI, 4083 S, CST) for 10 min. Images were acquired by fluorescence microscope (Nikon ECLIPSE Ci) or confocal microscope

(236) Somatostatin-evoked Aβ catabolism in the brain: Mechanistic involvement of α-endosulfine-K(ATP) channel pathwayMolecular psychiatryMarch 1, 2022Watamura N, Kakiya N, Nilsson P, Tsubuki S, Kamano N, Takahashi M, Hashimoto S, Sasaguri H, Saito T, Saido TC.tyramide-enhanced FITC or rhodamine for 10 min. Finally, sections were treated with DAPI (Cell Signaling Technology 4083 S) diluted in TNB buffer before mounting with PermaFluor (Thermo Fisher Scientific TA-030-FM). Sections

(237) Diverse Macrophages Constituted the Glioma Microenvironment and Influenced by PTEN StatusFrontiers in ImmunologyFebruary 21, 2022Fengqi Zhou et al.for 1 h at room temperature, counterstained with 4,6-dianmino-2-phenylindole (DAPI) (Cell Signaling, 4083, 1:5,000), and sealed with mounting medium (Sigma-Aldrich, F4680). Statistical Analysis The differences

(238) Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinomaCell death & diseaseFebruary 17, 2022Ling Z, Li W, Hu J, Li Y, Deng M, Zhang S, Ren X, Wu T, Xia J, Cheng B, et al.incubation in the dark at room temperature. The cells were then counterstained with 0.5 ug/mL of DAPI (#4083 s, CST, Danvers, MA, USA) for 5 min at room temperature and washed with PBS three times. The samples

(239) DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3JCI insightFebruary 8, 2022Borza CM, Bolas G, Bock F, Zhang X, Akabogu FC, Zhang MZ, de Caestecker M, Yang M, Yang H, Lee E, et al.antibody (1:1000, Abcam, ab225225) for 12 hours at 4°C, then incubated with DAPI (1:200, Cell Signaling, 4083) in PBS for 10 minutes at room temperature. Slides were mounted with Prolong Gold Antifade reagent (Invitrogen,

(240) Exogenous melatonin prevents type 1 diabetes mellitus-induced bone loss, probably by inhibiting senescenceOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USAFebruary 1, 2022Gong Z, Da W, Tian Y, Zhao R, Qiu S, Wu Q, Wen K, Shen L, Zhou R, Tao L, et al.at room temperature for 1 h. After washing with cold PBS 3 times, the cells were stained with DAPI (4083, 0.1 μg/ml, CST, America) for 5 min. After washing with cold PBS 3 times, images were captured with

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DAPI

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