DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(221) Inhibition of the LRRC8A channel promotes microglia/macrophage phagocytosis and improves outcomes after intracerebral hemorrhagic strokeiScienceDecember 22, 2022Liu J, Shen D, Wei C, Wu W, Luo Z, Hu L, Xiao Z, Hu T, Sun Q, Wang X, et al.TRIzol reagent,Invitrogen,15596018 DMEM/F12,Gibco,11039021 Penicillin/streptomycin,Sigma-Aldrich,V900929 DAPI,Cell Signaling Technology,4083S DCPIB,Tocris,1540/10 Hemin,Frontier Scientific,7084–24–4 ML385,Selleck,S8790 RIPA Lysis buffer,Boster,AR0101 30%

(222) Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human MacrophagesPharmaceuticsDecember 14, 2022Wikan N, Potikanond S, Hankittichai P, Thaklaewphan P, Monkaew S, Smith DR, Nimlamool W.secondary antibody (1:400), 1 μg/mL of 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cat No. 4083) (Cell Signaling Technology, Danvers, MA, USA) for nuclei staining, and with 1× of DyLight TM 594-Phalloidin

(223) Hepatic PLIN5 Deficiency Impairs Lipogenesis through Mitochondrial DysfunctionInternational journal of molecular sciencesDecember 9, 2022Zhang E.imaging. Nuclei were stained with DAPI (4′, 6-diamidino-2-phenylindole) (Cell Signaling, Danvers, MA, USA, 4083S) for 10 min followed by mounting onto slides for visualization. Images from 6 different fields per well

(224) The NALCN channel regulates metastasis and nonmalignant cell disseminationNature geneticsDecember 1, 2022Rahrmann EP, Shorthouse D, Jassim A, Hu LP, Ortiz M, Mahler-Araujo B, Vogel P, Paez-Ribes M, Fatemi A, Hannon GJ, et al.Thermo Fisher Scientific, 1:500). Sections were counterstained (4,6-diamidino-2-phenylindole (DAPI); catalog number 4083, Cell Signaling, 1:10,000) and images captured using a Zeiss ImagerM2 and Apotome microscope or Zeiss

(225) Chemically defined and small molecules-based generation of sinoatrial node-like cellsStem Cell Research & TherapyDecember 1, 2022Xiaojie Hou et al.Additional file 2: Table S2. Wash with PBS three times before each step. Nuclei were stained with DAPI (4083, Cell Signaling Technology, USA) for 15 min at room temperature. Fluorescence images were captured by

(226) Maternal rodent exposure to di-(2-ethylhexyl) phthalate decreases muscle mass in the offspring by increasing myostatinJournal of cachexia, sarcopenia and muscleDecember 1, 2022Li F, Luo T, Rong H, Lu L, Zhang L, Zheng C, Yi D, Peng Y, Lei E, Xiong X, et al.dilution, Invitrogen) 1 h at RT, followed by nuclear staining with 4′6‐diamidino‐2‐phenylindole (cat# 4083, CST). The TA muscle sections (5 μm) were stained with anti‐laminin antibody (cat# L0663, Sigma) for

(227) Anti-TGF-β/PD-L1 bispecific antibody promotes T cell infiltration and exhibits enhanced antitumor activity in triple-negative breast cancerJournal for immunotherapy of cancerDecember 1, 2022Yi M, Wu Y, Niu M, Zhu S, Zhang J, Yan Y, Zhou P, Dai Z, Wu K.1:300) was incubated at room temperature for 1 hour. The cell nuclei were counterstained with DAPI (4083, CST, 1 µg/mL). Western blotting Western blotting was performed as previously described.26 The following

(228) Albumin alleviated esketamine-induced neuronal apoptosis of rat retina through downregulation of Zn2+-dependent matrix metalloproteinase 9 during the early developmentBMC NeuroscienceNovember 16, 2022Kan Zhang, Ruijing Ma, Luping Feng, Peiwen Liu, Shuang Cai, Chaoyang Tong, Jijian ZhengAlexa Fluor 488 (1:500, Abcam ab150109). Sections were incubated with DAPI (1:1000, Cell Signaling 4083 S) for 15 min. Fluorescent images were visualized using a confocal microscope (Leica TCS SP8). Images

(229) PPAR agonists attenuate lenalidomide's anti-myeloma activity in vitro and in vivoCancer lettersOctober 1, 2022Sha Y, Wu J, Paul B, Zhao Y, Mathews P, Li Z, Norris J, Wang E, McDonnell DP, Kang Y.thrice with PBS, the slides were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 4083; Cell Signaling) for 5 min and mounted with the antifade mounting medium (Vector, H-1000). Images were

(230) FBXO42 facilitates Notch signaling activation and global chromatin relaxation by promoting K63-linked polyubiquitination of RBPJScience AdvancesSeptember 23, 2022Hua Jiang, Weixiang Bian, Yue Sui, Huanle Li, Han Zhao, Wenqi Wang, Xu LiRRID:AB_2630356) at room temperature for 1 hour, and subjected to 4′,6-diamidino-2-phenylindole (DAPI) staining (4083S, CST). Coverslips were mounted using FluorSave reagent (345789, Millipore). The cells were viewed using

(231) Targeting the succinate receptor effectively inhibits periodontitisCell reportsSeptember 20, 2022Guo Y, Xu F, Thomas SC, Zhang Y, Paul B, Sakilam S, Chae S, Li P, Almeter C, Kamer AR, et al./14190-136 Succinate,Sigma,14160-100G EDTA,Fisher Chemical,E478-1 BlockAid™ Blocking Solution,Invitrogen,B10710 DAPI,Cell Signaling Technology,4083 ActinGreen™ 488,Invitrogen,R37110 Sodium acetate,Sigma,S2889 L-(+) Tartaric acid,Sigma,T6521 Glacial

(232) AKR1C1 Protects Corneal Epithelial Cells Against Oxidative Stress-Mediated Ferroptosis in Dry EyeInvestigative Opthalmology & Visual ScienceSeptember 6, 2022Xin Zuo, Hao Zeng, Bowen Wang, Xue Yang, Dalian He, Li Wang, Hong Ouyang, Jin Yuananti–rabbit IgG (#4412), Alexa Fluor 488-labeled donkey anti-mouse IgG (#4409) antibodies, and DAPI (#4083) were purchased from Cell Signaling Technology (Danvers, MA, USA). The following antibodies were purchased

(233) A positive mechanobiological feedback loop controls bistable switching of cardiac fibroblast phenotypeCell discoverySeptember 6, 2022Niu L, Cheng B, Huang G, Nan K, Han S, Ren H, Liu N, Li Y, Genin GM, Xu F.by Rhodamine phalloidin (1:500; Invitrogen, R415, USA). DAPI (0.5 μg/mL; Cell Signaling Technology, 4083, USA) was used to stain cell nuclei. Immunofluorescent staining for YAP/F-actin/DAPI was used to calculate

(234) The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSCInternational journal of stem cellsAugust 30, 2022Liu F, Long D, Huang W, Peng W, Lan H, Zhou Y, Dang X, Zhou R.Invitrogen, USA). The cells were washed 3 times with PBS and then counterstained using 0.5 μg/ml of DAPI (4083, Cell Signaling Technology, USA) for 15 min at RT. The chambers were mounted and visualized under fluorescence microscopy

(235) Quinolizidines as Novel SARS-CoV-2 Entry InhibitorsInternational Journal of Molecular SciencesAugust 25, 2022Li Huang, Lei Zhu, Hua Xie, Jeffery Shawn Goodwin, Tanu Rana, Lan Xie, Chin-Ho Chenwith Prolong® Gold Anti-Fade Reagent with DAPI (Cell Signaling Technology, Danvers, MA, USA, Cat. # 4083S). Confocal images were acquired using a Nikon TE2000-U laser-scanning confocal microscope (Nikon, Tokyo,

(236) Transplantation of insulin-producing cells derived from human mesenchymal stromal/stem cells into diabetic humanized miceStem cell research & therapyJuly 26, 2022Ghoneim MA, Gabr MM, Refaie AF, El-Halawani SM, Al-Issawi MM, Elbassiouny BL, Kader MAAE, Ismail AM, Zidan MF, Karras MS, et al.anti-rabbit IgG (H + L) (# 4413, Cell Signaling Technology). The nuclei were counterstained with DAPI (# 4083, Cell Signaling Technology). For intracellular staining for flow cytometry: Primary monoclonal antibodies

(237) Single-cell RNA sequencing of subcutaneous adipose tissues identifies therapeutic targets for cancer-associated lymphedemaCell discoveryJune 21, 2022Liu X, Yuan M, Xiang Q, Li Z, Xu F, Chen W, Chen J, Huang J, Yu N, Zhou Z, et al.Fluor 488 goat anti-rabbit (A-11008, Invitrogen) secondary antibody at room temperature for 1 h. DAPI (4083S, Cell Signaling Technology) was used for nuclear counterstaining. The cells were imaged using the Axio

(238) A novel frameshift variant in the TSPAN12 gene causes autosomal dominant FEVRMolecular genetics & genomic medicineJune 1, 2022Peng L, Dai E, Xiao H, Zhao R, He Y, Li S, Yang M, Yang Z, Zhao P.Plus 488 secondary antibody (1:300 dilution; CAT# A32723; Invitrogen) and DAPI (1:500 dilution; CAT# 4083; Cell Signaling Technology). Images were captured on an LSM 800 confocal scanning microscope (Zeiss,). 3

(239) miR-181b regulates vascular endothelial aging by modulating a MAP3K3 signaling pathwayFASEB JJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G McCoy, Daniel Pérez-Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtemperature. Nuclei were stained for 5min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5μg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen). Images were acquired

(240) miR‐181b regulates vascular endothelial aging by modulating an MAP3K3 signaling pathwayThe FASEB JournalJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G. McCoy, Daniel Pérez‐Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtemperature. Nuclei were stained for 5min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5μg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen). Images were acquired

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DAPI

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