DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(261) The histone chaperone Anp32e regulates memory formation, transcription, and dendritic morphology by regulating steady-state H2A.Z binding in neuronsCell ReportsAugust 17, 2021Gilda Stefanelli et al.Fluor 488, A11039, 1:5000) for 1 h at RT and washed. DNA was counter stained with DAPI (Cell Signaling, 4083S). Slides were mounted with ProLong Gold Antifade Reagent (Cell Signaling, 9071). Images were acquired

(262) Survival motor neuron deficiency slows myoblast fusion through reduced myomaker and myomixer expressionJournal of cachexia, sarcopenia and muscleAugust 1, 2021McCormack NM, Villalón E, Viollet C, Soltis AR, Dalgard CL, Lorson CL, Burnett BG.additional 5 days. Cells on coverslips were fixed with 4% paraformaldehyde and stained with DAPI (Cat. #4083S, Cell Signalling Technology, Danvers, MA, USA). Five images per condition were quantified for each n.

(263) SENP1 promotes MCL pathogenesis through regulating JAK-STAT5 pathway and SOCS2 expressionCell death discoveryJuly 26, 2021Zhang Y, Ma Y, Wu G, Xie M, Luo C, Huang X, Tian F, Chen J, Li X.p53 (#9282), STAT5 (#94205), p-STAT5 (#4322 and #9359), LaminB1 (#13435), Ki67 (#12202) and DAPI (#4083) were obtained from Cell Signaling Technology (Shanghai, China). Recombinant human erythropoietin (EPO)

(264) The effect of young and old ex vivo human serum on cellular protein synthesis and growth in an in vitro model of agingAmerican journal of physiology. Cell physiologyJuly 1, 2021Allen SL, Marshall RN, Edwards SJ, Lord JM, Lavery GG, Breen L.MA) for 1 h in darkness. Cells were washed once with PBS and incubated in the dark with DAPI (1:5,000, 4083, Cell Signaling Technology, Leiden, Netherlands) for 5 min. Finally, cells were washed with PBS before

(265) YWHAE as an HE4 interacting protein can influence the malignant behaviour of ovarian cancer by regulating the PI3K/AKT and MAPK pathwaysCancer cell internationalJune 9, 2021Li X, Wang C, Wang S, Hu Y, Jin S, Liu O, Gou R, Nie X, Liu J, Lin B.which was then incubated for 2 h in the dark. Nuclei were stained with 4′,6-diamidino-2-phenylindole (4083S; Cell Signaling Technology Inc., Danvers, MA), and an anti-quenching agent was added dropwise onto the

(266) PARP inhibition promotes ferroptosis via repressing SLC7A11 and synergizes with ferroptosis inducers in BRCA-proficient ovarian cancerRedox BiologyJune 1, 2021Ting Hong, Guang Lei, Xue Chen, He Li, Xiaoye Zhang, Nayiyuan Wu, Yu Zhao, Yilei Zhang, Jing Wang

(267) Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell functionDiabetologiaMay 1, 2021Zhang M, Yang C, Zhu M, Qian L, Luo Y, Cheng H, Geng R, Xu X, Qian C, Liu Y.anti-phosphoPI3 kinase p85 (Tyr458)/p55 (Tyr199) (catalogue no. #17366) antibodies and DAPI (catalogue no. #4083) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAP SH3 binding protein 1 (G3BP1)

(268) Curcumin Alleviates Chronic Pain and Improves Cognitive Impairment via Enhancing Hippocampal Neurogenesis in Sciatic Nerve Constriction RatsJournal of Pain ResearchApril 20, 2021Jingyi Du, Yifan Deng, Zhuolin Qiu, Guoliang Sun, Yue Guo, Ziqing Hei, Xiang Li(1:500, ab150081, Abcam). Then slices were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI, 0.5µg/mL, 4083s, Cell Signaling Technology). After staining, slices were visualized by two experimenters blinded to

(269) The m⁶A methylome of SARS-CoV-2 in host cellsCell researchApril 1, 2021Liu J, Xu YP, Li K, Ye Q, Zhou HY, Sun H, Li X, Yu L, Deng YQ, Li RT, et al.overnight with 5% donkey serum and 0.15% Triton X-100. Nuclei were counterstained with DAPI DNA dye (CST, 4083, 1:1000) at RT for 10 min and mounted on glass slides. Images were taken using a PerkinElmer High Content

(270) FoxA2 and RNA Pol II mediate human islet amyloid polypeptide turnover in ER-stressed pancreatic β-cellsThe Biochemical journalMarch 26, 2021Chatterjee Bhowmick D, Burnett L, Kudaibergenova Z, Jeremic AM.minutes at RT. Nuclei were stained with nuclear-specific dye, DAPI (Cell Signaling Technology, cat# 4083) for 10 min at RT. The following antibodies (Abcam) were used: ER marker, anti-calnexin (1 : 500, cat#

(271) Ruscogenin attenuated tight junction injury and tumor migration in colorectal liver metastasis mice via regulating TRAP1Translational cancer researchMarch 1, 2021Lv Y, Wu X, Chen J, Shao F.nuclei of all cells were then washed and incubated with 4’,6-diamidino-2-phenylindole (DAPI, CST, USA, 4083, 1:1,000) for 15 min at room temperature. Briefly, confocal images were converted to 8-bit format, and

(272) Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosisAutophagyMarch 1, 2021Cui W, Sathyanarayan A, Lopresti M, Aghajan M, Chen C, Mashek DG.live-cell imaging. Nuclei were stained with DAPI (4′, 6-diamidino-2-phenylindole) (Cell Signaling, 4083S) for 10 min followed by mounting onto slides for visualization. To evaluate the specificity of the CD107

(273) Zinc ameliorates human aortic valve calcification through GPR39 mediated ERK1/2 signalling pathwayCardiovascular researchFebruary 22, 2021Chen Z, Gordillo-Martinez F, Jiang L, He P, Hong W, Wei X, Staines KA, Macrae VE, Zhang C, Yu D, et al.hVICs were determined by manually counting pyknotic nuclei after staining with DAPI (Cell Signaling, 4083S). In addition, hVICs in different stages of apoptosis were analyzed by flow cytometry using Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI

(274) N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional EffectsJournal of clinical immunologyFebruary 1, 2021Nunes-Santos CJ, Kuehn HS, Rosenzweig SD.A-11072). Next, cells were washed twice with PBS and incubated with DAPI (Cell Signaling technology, Cat. 4083S) in PBS for 10 min, followed by 2 additional washes with PBS. Representative images (range, 8–20) per

(275) STOML2 potentiates metastasis of hepatocellular carcinoma by promoting PINK1-mediated mitophagy and regulates sensitivity to lenvatinibJournal of hematology & oncologyJanuary 14, 2021Zheng Y, Huang C, Lu L, Yu K, Zhao J, Chen M, Liu L, Sun Q, Lin Z, Zheng J, et al.were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Cell Signaling Technology, #4083) at RT for 10 min. Photographs were captured with a laser confocal microscopy (Leica Microsystems AG).

(276) A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus ResponseFrontiers in immunologyJanuary 1, 2021Li X, Dong Z, Liu Y, Song W, Pu J, Jiang G, Wu Y, Liu L, Huang X.were added and incubated for 2h at RT. Nuclei were subsequently visualized with DAPI (1:2000) (CST, 4083) and cells were imaged and quantified using the laser confocal microscope (LSM880; Zeiss, Germany).

(277) Direct SARS-CoV-2 infection of the human inner ear may underlie COVID-19-associated audiovestibular dysfunctionCommun Med (Lond)January 1, 2021Jeong M, Ocwieja KE, Han D, Wackym PA, Zhang Y, Brown A, Moncada C, Vambutas A, Kanne T, Crain R, et al.(Supplementary Table 3) in PBST. Vectashield (Vector Laboratories, Cat# H1000) with DAPI (Cell Signaling, Cat# 4083) was used to mount the samples and visualize cellular nuclei. Negative control experiments without the

(278) A Novel Bispecific Antibody Targeting PD-L1 and VEGF With Combined Anti-Tumor ActivitiesFrontiers in immunologyJanuary 1, 2021Cui X, Jia H, Xin H, Zhang L, Chen S, Xia S, Li X, Xu W, Chen X, Feng Y, et al.were performed sequentially. After the antigen staining cycle, DAPI (4, 6-diamino-2-phenyl indole, 4083, Cell Signaling Technology) solution was added. The information for the primary and secondary antibodies

(279) Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cellseLifeDecember 17, 2020Gumber D, Do M, Suresh Kumar N, Sonavane PR, Wu CCN, Cruz LS, Grainger S, Carson D, Gaasterland T, Willert K.compound, drug D-luciferin-Potassium Salt ThermoFisher Scientific Cat# 50227 Other DAPI stain Cell Signaling Technology Cat# 4083S Other Protein G Sepharose BioVision Cat# 6511 Other HiTrap IMAC HP, 1 mL Cytiva Cat# 17092003 Other Superdex 200 10/300 GL

(280) Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3ζ co-knockdownOncology ReportsDecember 14, 2020Tahereh Kashkoulinejad-Kouhi, Shahrokh Safarian, Blanca Arnaiz, Laura Saa300 µl of 7-AAD/RNase solution was added at the end. 4',6-Diamidino-2-phenylindole (DAPI; product no. 4083; Cell Signaling Technology, Inc.) at 1 µg/ml prepared in PBS was applied to stain nucleus. The cells

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DAPI

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