RNA 5 Pyrophosphohydrolase (RppH) M0356S from New England Biolabs

Product Specs
ItemRNA 5 Pyrophosphohydrolase (RppH)
CompanyNew England Biolabs
Price $103.00 Inquire
Catalog NumberM0356S
Size200 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Chromatin-intrinsic mechanisms determine orientation-specific class switch recombinationNature CommunicationsFebruary 28, 2026Sha Luo, Ruolin Qiao, Hailiang Zha, Leyi Yu, Xiaoling Shan, Shuchan Li, Zifan Yang, Aoyu Ma, Yaxin Cheng, Yao-Feng Zhao, et al.enriched run-on samples were incubated with hydroxyl repair with T4 PNK (NEB, M0201S) and RppH (NEB, M0356S), followed by ligating the 5′ and 3′ RNA adaptor. RT-PCR was performed from the adaptor-ligated RNA

(2) NAD+ capping of sibD transcripts in E. coli is mediated by its minimal promoter and enhanced by ppGppNucleic Acids ResearchFebruary 24, 2026Wuzhen Liu, Kefan Hu, Shiqi Nie, Zhang Feng, Qiongfang Li, Hailei Zhang, Shumin Liang, Jialin Peng, Shoudong Zhang, Zongwei Cai, et al.subjected to a decapping assay by incubating 20 ng of RNA with 0.5 μl NudC (M0607S, NEB) and 0.5 μl RppH (M0356S, NEB). For SibD RNAs, the decapped SibD RNAs were circularized with 30 U T4 RNA ligase (Thermo Fisher)

(3) Cell-type-specific RNA polymerase II activity maps in intact tissues provide a gateway to mammalian gene regulatory mechanisms in vivoDevelopmental CellFebruary 11, 2026Gopal Chovatiya, Sean Y. Huang, Alex B. Wang, Philip Versluis, Chris K. Bai, Michael DeBerardine, Yu-Ching Liao, Judhajeet Ray, Abdullah Ozer, John T. Lis, et al.resuspending beads with nascent RNA in RppH reaction mix (10x ThermoPol buffer (NEB, B9004S), RppH (NEB, M0356S), SUPERase-In RNase Inhibitor) and incubating at 37°C for 45 minutes on thermomixer at 500 rpm. Following

(4) Profiling active RNA polymerase II transcription start sites from total RNA by capped small RNA sequencing (csRNA-seq)Nature ProtocolsJanuary 16, 2026Mackenzie K. Meyer, Oluwadamilola J. Olanrewaju, Patricia Montilla-Perez, Anna L. McDonald, Eva M. Rickard, Francesca Telese, Christopher Benner, Marina I. Savenkova, Sascha H. Duttke(New England Biolabs, cat. no. M0525L) RNA 5’ Pyrophosphohydrolase (RppH, New England Biolabs, cat. no. M0356S) 2-Propanol (Sigma-Aldrich, cat. no. I9516–25ML) ! CAUTION: Highly flammable liquid and vapor. Causes

(5) A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memoryNature CommunicationsJanuary 9, 2026Chenming Zeng, Giulia Furlan, Miguel Vasconcelos Almeida, Juan C. Rueda-Silva, Jonathan Price, Helena Santos-Rosa, Jingxiu Xu, Yan Kuang, Enric Cata Socias, Jonas Mars, et al.two-sided unpaired t-test. small RNA-seq and sequencing analysis Total RNAs were treated with RppH (NEB #M0356S)130, and small RNA libraries were prepared with a small RNA-seq Kit v4 with UDIs (Nextflex #NOVA-5132-31).

(6) Hypoxia leads to reduced mito-nuclear gene expression and increased mtDNA transcriptional pausing in human cellsCommunications BiologyJanuary 8, 2026Noam Shtolz, Sarah Dadon, Dan Mishmarrepaired with RNA 5′ pyrophosphohydrolase, followed by phosphorylation with T4 polynucleotide kinase (M0356S and M0201S, both from NEB). Next, the 5′ ends of the RNA molecules were ligated to the Rev5 RNA adapter

(7) Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycleMolecular CellDecember 18, 2025Aviezer Silverman, Raneem Nashef, Reut Wasserman, Tamar Noy, Susan Born, Tianyou Yao, Yuncong Geng, Hila Rotbard, Adi Levkowitz, Yotam Kaufman, et al.inhibitor,Takara,Cat#2313A RNase T1,Boehringer,N/A DMS,Fluka,Cat#41610 RppH,New England Biolabs,Cat#M0356S 5′-Phosphate-Dependent Exonuclease (TeX),Lucigen,Cat#TER51020 5-bromo-2′-deoxyuridine (BrdU),Sigma Aldrich

(8) The Incoming Influenza Genome Assembles a Host RBP Network that Orchestrates Viral RNA SynthesisbioRxivDecember 11, 2025Stephen Clarke, Deep B. Patel, Andrea Ascura, Caroline L. Wiser, Jennifer L. Pennise, Samantha M. Lisy, Manuel AscanoUniversity),N/A T4 DNA ligase,New England Biolabs,M0202T RNA 5’Pyrophosphohydrolase (RppH),New England Biolabs,M0356S "RNase inhibitor, Murine",New England Biolabs,M0314S DNase I (RNase free),New England Biolabs,M0303S TPCK-trypsin,Thermo Fischer Scientific

(9) A new role for PHYHD1 and related dioxygenases: demethylation of 2'-O-methylated nucleosidesNucleic Acids ResDecember 11, 2025Justas Stonkus, Rasa Rutkienė, Rita Meškienė, Martyna Jasiūnienė, Agota Aučynaitė, Laura Kalinienė, Justas Lazutka, Darius Balčiūnas, Giedrius Vilkaitis, Rolandas Meškyshydrolysis Purified mRNA was first incubated with RNA 5′ pyrophosphohydrolase (RppH, New England Biolabs, USA, #M0356S) in NEB buffer 2 according to the manufacturer’s instructions. Total RNA and decapped mRNA were first

(10) CTCF couples long-range loop extrusion and diffusion to mediate a diverse Igκ repertoireNature CommunicationsDecember 11, 2025Emma L. Bush, Brigette Berke-Reynolds, Kaitlyn M. Hutchins, Xinrui Yu, Jorge A. Colón-Rosado, Fujung Chang, John Curran, Jiaxin Yang, Liangliang Sun, Jianrong Wang, et al.Dynabeads MyOne Streptavidin C1 beads, treated with RNA 5’ Pyrophosphohydrolase (New England Biolabs, M0356S) and T4 Polynucleotide Kinase (New England Biolabs, M0201S), and sequentially ligated to 5’ and 3’ RNA

(11) RG motifs promote piRNA-mediated gene silencing in C. elegansNucleic Acids ResDecember 11, 2025Dylan C Wallis, Carolyn M Phillipspolyphosphatase (Epicentre, RP8092H) (total small RNA samples) or 5′ RNA pyrophosphohydrolase (NEB M0356S) (IP samples) and ligated to 3′ pre-adenylated adapters with truncated T4 RNA ligase (NEB M0373L). Small

(12) Argonaute-siRNA loading via the RNA-binding protein RDE-4 in C. elegansCurrent BiologyDecember 1, 2025Thiago L. Knittel, Brooke E. Montgomery, Reese A. Sprister, Colin N. Magelky, Margaret J. Smith, Maritza Soto-Ojeda, Melissa Guthrie, Carolyn M. Phillips, Taiowa A. Montgomeryby Thermo Fisher Scientific",Cat# NW04127BOX RNA 5′ Pyrophosphohydrolase,New England Biolabs,Cat# M0356S Alt-R™ S.p. Cas9 Nuclease V3,Integrated DNA Technologies,Cat# 1081058 Critical commercial assays,Critical

(13) TAF1 is required for fetal but not adult hematopoiesis in miceDevelopmental CellNovember 3, 2025Fan Liu, Jingyin Yue, Francesco Tamiro, Jun Sun, Pradeep Kumar Reddy Cingaram, Krystal Lisa Hossack, Concepcion Martinez Caja, Ye Xu, Chuan Chen, Felipe Beckedorff, et al.purified by streptavidin beads M-280 (Invitrogen, 11205D), and the 5' cap was removed using RppH (NEB, M0356S). T4 polynucleotide kinase (NEB, M0201) was then used to repair the 5' hydroxyl terminus. Following

(14) Argonautes and small RNAs associated with nematode programmed DNA eliminationbioRxivOctober 27, 2025Maxim V. Zagoskin, Yuanyuan Kang, Richard E. Davis, Jianbin Wang°C). To enable cloning of siRNAs carrying 5′ triphosphates, samples were treated with RppH (NEB, catalog #M0356S) in ThermoPol buffer for 1 h at 37 °C to remove cap and convert 5′ di- and triphosphates to monophosphates

(15) Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiont WolbachiaMicrob GenomOctober 27, 2025Youseuf Suliman, Zhiru Li, Amit Sinha, Philip D. Dyer, Catherine S. Hartley, Laurence Ettwiller, Alistair C. Darby, Clotilde K. Carlow, Benjamin L. MakepeaceRNA to 2.2 µl of 10X ThermoPol buffer (NEB product number B9004) and 2 µl RppH (NEB product number M0356S), followed by incubation at 37 °C for 1 h. Next, 1 µl of 1 : 10 diluted proteinase K was added to the

(16) The MLLT3 YEATS domain is a dual reader of histone marks (H3K9/18/27ac/cr) and ncRNA (7SK), linking epigenetic and RNA signaling to regulate hematopoiesisbioRxivSeptember 11, 2025Adam Boulton, Ashish Kabra, Nicholas Achille, Emmalee R. Adelman, Dimitrios G. Anastasakis, Felipe Beckedorff, Pradeepkumar Cingaram, Ying Zhang, Benjamin Leach, Ramin Shiekhattar, et al.purified by streptavidin beads M-280 (Invitrogen, 11205D), and the 5’ cap was removed using RppH (NEB, M0356S). T4 polynucleotide kinase (NEB, M0201) was then used to repair the 5’ hydroxyl terminus. Following

(17) Integrator promotes the association of TFIID and RNA polymerase II to maintain pluripotency during developmentMolecular CellAugust 7, 2025Raghu Ram Edupuganti, Felipe Beckedorff, Pradeep Kumar Reddy Cingaram, Saeid Mohammad Parast, Jingyin Yue, Gary LeRoy, Monica Guiselle Valencia, Sadat Dokaneheifard, Helena Gomes Gomes Dos Santos, Omar Lopez Ocejo, et al.Gold,InvivoGen,ant-hg-1 Lipofectamine 3000,Invitrogen,100022052 Biotin-11-NTPs,PerkinElmer,NEL54(2/3/4/5)001 RppH,NEB,M0356S PNK,NEB,M0201L SuperScript III,Invitrogen,18080051 Salmon Sperm DNA,Thermo Scientific,15632011 TSKgel-DEAE-5PW,TOSO

(18) Biochemical analysis of human eIF4E-DCP2 interaction: Implications for the relationship between translation initiation and decappingPLoS OneAugust 1, 2025Zachary F. Mandell, Jeff Collerincubating the 5’ triphosphate RNA oligos with the bacterial RNA 5’ pyrophosphohydrolase, RppH (NEB cat # M0356S), then purified using silica-membrane spin columns. Each of the RNA oligos was of the sequence 5’-AGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUAAGAGC-3’. Recombinant

(19) Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposonsNat CommunJuly 2, 2025KeHuan K. Edmonds, Max E. Wilkinson, Daniel Strebinger, Hongyu Chen, Blake Lash, Clarissa C. Schaefer, Shiyou Zhu, Dangliang Liu, Shai Zilberzwige-Tal, Alim Ladha, et al.RP-HPLC. For 5’ modification, in vitro transcribed RNA (uncapped) were first digested with RppH (NEB, M0356S) to yield 5P-mRNA, which were then ligated to chemically synthesized (and capped) oligonucleotides with

(20) Chemical and topological design of multicapped mRNA and capped circular RNA to augment translationNature BiotechnologyJuly 1, 2025Hongyu Chen, Dangliang Liu, Abhishek Aditham, Jianting Guo, Jiahao Huang, Franklin Kostas, Kamal Maher, Mirco J. Friedrich, Ramnik J. Xavier, Feng Zhang, et al.oligonucleotides to mRNAs The 5′-triphosphorylated mRNA generated by IVT was first treated with RppH (NEB, M0356S) according to the manufacturer’s protocol to generate 5P-mRNA and purified with the Monarch RNA cleanup

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RNA 5 Pyrophosphohydrolase (RppH)

RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

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RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

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