RNA 5 Pyrophosphohydrolase (RppH) M0356S from New England Biolabs

Product Specs
ItemRNA 5 Pyrophosphohydrolase (RppH)
CompanyNew England Biolabs
Price $103.00 Inquire
Catalog NumberM0356S
Size200 units
ApplicationsFunctional Genomics
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cellsCell DiscovMarch 18, 2025Lili Li, Xiaoyin Tang, Xuanxuan Guo, Di Rao, Lin Zeng, Junchao Xue, Shuxian Liu, Shikui Tu, En-Zhi Shenprocedure was performed as described previously98. Briefly, RNA 5′ Pyrophosphohydrolase (RppH) (NEB, M0356S) was used to trim the 5′ triphosphate of endogenous 22G-RNAs to monophosphate. The 3′ and 5′ adapters

(42) The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expressionJournal of VirologyFebruary 25, 2025Ruth Verhamme, Robert J. J. Jansens, Jianheng Liu, Fien Van Raemdonck, Cliff Van Waesberghe, Luke Nicholson, Samie R. Jaffrey, Herman W. Favoreelenrichment, the RNA fragments were eluted into water. To ligate the 5′ adapter, we used RppH (NEB #M0356S) to release the 3′-Desthiobiotin caps, which results in monophosphate 5′ ends ready for ligation. We

(43) Decreased SynMuv B gene activity in response to viral infection leads to activation of the antiviral RNAi pathway in C. elegansPLoS BiolJanuary 29, 2025Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H. X. Liu, Jennifer D. Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J. Tate, Taiowa A. Montgomery, Gary Ruvkuntriphosphates to monophosphates following the manufacturer’s recommendations (New England Biolabs, cat# M0356S) [77]; 16 to 30-nt small RNAs were size selected on a 17% polyacrylamide/urea gel and purified using

(44) 5′ terminal nucleotide determines the immunogenicity of IVT RNAsNucleic Acids ResearchJanuary 24, 2025Magdalena Wolczyk, Jacek Szymanski, Ivan Trus, Zara Naz, Tola Tame, Agnieszka Bolembach, Nila Roy Choudhury, Karolina Kasztelan, Juri Rappsilber, Andrzej Dziembowski, et al.different conditions – without enzyme, with XRN-1 (NEB #M0338S) alone, or with XRN-1 and RppH (NEB #M0356S) together. Reactions were performed at 37°C for 60 min in dedicated buffer for XRN-1 enzyme provided

(45) Noncoding RNA Terc-53 and hyaluronan receptor Hmmr regulate aging in miceProtein & CellJanuary 4, 2025Sipeng Wu, Yiqi Cai, Lixiao Zhang, Xiang Li, Xu Liu, Guangkeng Zhou, Hongdi Luo, Renjian Li, Yujia Huo, Zhirong Zhang, et al.TRIzol reagent. In vitro transcribed RNAs were treated with RNA 5’ pyrophosphohydrolase (RppH, NEB, Cat# M0356S) to remove pyrophosphate from the RNAs, and then purified by ethanol precipitation. RNA isolation RNAs

(46) Aptamer-based assay for high-throughput substrate profiling of RNA decapping enzymesNucleic Acids ResearchNovember 27, 2024Katarzyna Grab, Mateusz Fido, Tomasz Spiewla, Marcin Warminski, Jacek Jemielity, Joanna Kowalskadescribed (48). EcRppH and 5′-Polyphosphatase were purchased from New England Biolabs (catalog number M0356S) and Lucigen (catalog number RP8092H), respectively. Design of aptamer probes DNA oligonucleotide

(47) Uncovering the transcriptome-wide RNA modifications in Acinetobacter baumanniiMicrobial GenomicsNovember 20, 2024Kah Ern Ten, Sadequr Rahman, Hock Siew Tanpoly(A)-tailed total RNA of Ab-C98 was dephosphorylated by RNA 5'-pyrophosphohydrolase (RppH) (NEB, M0356S). The RNAs with 5’-monophosphate were ligated to the T7 promoter using T4 RNA ligase 1 (NEB, M0204S).

(48) Divergent molecular assembly and catalytic mechanisms between bacterial and archaeal RNase P in pre-tRNA cleavageProceedings of the National Academy of SciencesOctober 22, 2024Xiaoge Liang, Dian Chen, Aimin Su, Yu Liudescribed earlier, followed by digestion with 2 U/μL 5′-pyrophosphohydrolase enzyme (RppH, Cat. No. M0356S, NEB, USA) at 37 °C for 2 h to generate monoP-211nt-RNA with a 5′-mono-phosphate. The sequence of the

(49) Zika virus non-coding RNAs antagonize antiviral responses by PKR-mediated translational arrestNucleic Acids ResearchOctober 14, 2024Horacio M Pallarés, María Mora González López Ledesma, Santiago Oviedo-Rouco, Luciana A Castellano, Guadalupe S Costa Navarro, Ana J Fernández-Alvarez, María Josefina D’Andreiz, Victor Daniel Aldas-Bulos, Diego E Alvarez, Ariel A Bazzini, et al.with LiCl 7.5 M precipitation, and then 1.5 ug of RNA was treated with RNA Pyrophosphohydrolase (NEB, M0356S) following the manufacturer's instructions. Each RNA was transfected into A549 cells at a concentration

(50) PAR-dCLIP: Enabling detection of RNA binding protein target transcripts bound at 5′ termini through the incorporation of a decapping stepMethods in EnzymologyOctober 10, 2024Samantha Lisy, Katherine Rothamel, Yelena Perevalova-Pinzul, Manuel Ascano0.2",Sigma-Aldrich,P4682 Chloroform,Sigma-Aldrich,C2432 Isoamyl Alcohol (Molecular Biology),Fisher BioReagents,BP1150 RNA 5’ Pyrophosphohydrolase (RppH),NEB,M0356S 10X ThermoPol Reaction Buffer,NEB,B9004S T4 RNA Ligase 1,NEB,M0204L Adenosine 5’-triphosphate (ATP)

(51) RNA interacts with topoisomerase I to adjust DNA topologyMolecular CellSeptember 5, 2024Mannan Bhola, Kouki Abe, Paola Orozco, Homa Rahnamoun, Pedro Avila-Lopez, Elijah Taylor, Nefertiti Muhammad, Bei Liu, Prachi Patel, John F. Marko, et al.B9200S "Ribonucleic Acid, Yeast",Millipore,Cat# 55714 RNA 5' Pyrophosphohydrolase (RppH),New England Biolabs,Cat# M0356S RNase I,Thermo Fisher Scientific,Cat# AM2294 RNaseOUT™ Recombinant Ribonuclease Inhibitor,Invitrogen,Cat#

(52) Staphylococcal exoribonuclease YhaM destabilizes ribosomes by targeting the mRNA of a hibernation factorNucleic Acids ResearchAugust 27, 2024Anna Lipońska, Hyun Lee, Mee-Ngan F Yapadditional cleanup followed by Turbo™ DNase treatment. RNA was decapped with RppH (New England Biolabs; M0356S) for 30 min at 37°C. RNA circularization was performed with T4 RNA Ligase I (New England Biolabs M0204L)

(53) Caenorhabditis elegans SynMuv B gene activity is down-regulated during a viral infection to enhance RNA interferencebioRxivJuly 19, 2024Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H.X. Liu, Jennifer D. Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J. Tate, Taiowa A. Montgomery, Gary Ruvkuntriphosphates to monophosphates following the manufacturer’s recommendations (New England Biolabs, cat# M0356S)(75). 16–30-nt small RNAs were size selected on a 17% polyacrylamide/urea gel and purified using the

(54) The Upstream Sequence Transcription Complex dictates nucleosome positioning and promoter accessibility at piRNA genes in the C. elegans germ linePLOS GeneticsJuly 10, 2024Nancy Paniagua, C. Jackson Roberts, Lauren E. Gonzalez, David Monedero-Alonso, Valerie ReinkeDirect-zol RNA microprep kit (Zymo Research #R2062). The RNA was then treated with 1.5 μL RppH (NEB M0356S) in a total reaction volume of 20 μL for 1 h at 37°C, resuspended in 100 μL Trizol (Invitrogen) and

(55) Identification and in vitro characterization of UDP-GlcNAc-RNA cap-modifying and decapping enzymesNucleic Acids ResearchJune 10, 2024Frederik Weber, Nikolas Alexander Motzkus, Leona Brandl, Marvin Möhler, Andrijana Alempijevic, Andres Jäschkefor RNA ligation were PAGE purified prior to use. Streptavidin (N7021S), RNA 5′ pyrophosphohydrolase (M0356S), NEBuffer 2, NudC (M0607S), NEBuffer r3.1, low range ssRNA ladder (N0364S) and the Monarch RNA Cleanup

(56) Tdrd3-null mice show post-transcriptional and behavioral impairments associated with neurogenesis and synaptic plasticityProg NeurobiolFebruary 1, 2024Zhu X, Joo Y, Bossi S, McDevitt RA, Xie A, Wang Y, Xue Y, Su S, Lee SK, Sah N, et al.overnight. Then biotin RNA was enriched and extracted again. RNA was modified in 5’ end by RppH (NEB, #M0356S) and performed hydroxyl repair by T4 PNK (NEB, #M0201L). After being extracted by ZYMO RNA clean & concentrator

(57) Effects of Methionine Restriction from Different Sources on Sperm Quality in Aging MiceNutrientsNovember 15, 2023Yinghui Wu, Hao Li, Yueyue Miao, Jian Peng, Hongkui Weipurified using an mRNA extraction kit and the sperm cap structure was removed using RppH enzyme (NEB.M0356S). The samples were then cleaved into ribonucleotides using Nuclease S1. The samples were then treated

(58) PRO-IP-seq tracks molecular modifications of engaged Pol II complexes at nucleotide resolutionNature CommunicationsNovember 3, 2023Anniina Vihervaara, Philip Versluis, Samu V. Himanen, John T. Lisconducted on-beads. The bead-nascent-RNA-complexes were diluted in RNA 5' Pyrophosphorylase mix (NEB, M0356S) and incubated at 37 °C for 45 min. The 5'-hydroxyrepair was subsequently conducted by adding T4 polynucleotide kinase

(59) Condensate cooperativity underlies transgenerational gene silencingCell reportsAugust 29, 2023Du Z, Shi K, Brown JS, He T, Wu WS, Zhang Y, Lee HC, Zhang D.Protease Inhibitor Cocktail (50X),Promega,Cat#G6521 RNA 5’Pyrophosphohydrolase (RppH),New England Biolabs,Cat#M0356S Isopropyl-beta-D-thiogalactopyranoside (IPTG),Biotopped,Cat#I6070 CAS:367–93-1 1-Bromo-3-chloropropane

(60) Tdrd3-null mice show post-transcriptional and behavioral impairments associated with neurogenesis and synaptic plasticityResearch SquareMay 12, 2023XingLiang Zhu, Yuyoung Joo, Simone Bossi, Ross McDevitt, Aoji Xie, Yue Wang, Yutong Xue, Shuaikun Su, Seung Kyu Lee, Nirnath Sah, et al.overnight. Then biotin RNA was enriched and extracted again. RNA was modified in 5’ end by RppH (NEB, #M0356S) and performed hydroxyl repair by T4 PNK (NEB, #M0201L). After being extracted by ZYMO RNA clean & concentrator

Be the First to Write a Review!

RNA 5 Pyrophosphohydrolase (RppH)

RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

New England Biolabs

Citations (124)

Reviews

RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map