RNA 5 Pyrophosphohydrolase (RppH) M0356S from New England Biolabs

Product Specs
ItemRNA 5 Pyrophosphohydrolase (RppH)
CompanyNew England Biolabs
Price $103.00 Inquire
Catalog NumberM0356S
Size200 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) CTCF couples long-range loop extrusion and diffusion to mediate a diverse Igκ repertoireNature CommunicationsDecember 11, 2025Emma L. Bush, Brigette Berke-Reynolds, Kaitlyn M. Hutchins, Xinrui Yu, Jorge A. Colón-Rosado, Fujung Chang, John Curran, Jiaxin Yang, Liangliang Sun, Jianrong Wang, et al.Dynabeads MyOne Streptavidin C1 beads, treated with RNA 5’ Pyrophosphohydrolase (New England Biolabs, M0356S) and T4 Polynucleotide Kinase (New England Biolabs, M0201S), and sequentially ligated to 5’ and 3’ RNA

(22) RG motifs promote piRNA-mediated gene silencing in C. elegansNucleic Acids ResDecember 11, 2025Dylan C Wallis, Carolyn M Phillipspolyphosphatase (Epicentre, RP8092H) (total small RNA samples) or 5′ RNA pyrophosphohydrolase (NEB M0356S) (IP samples) and ligated to 3′ pre-adenylated adapters with truncated T4 RNA ligase (NEB M0373L). Small

(23) The Incoming Influenza Genome Assembles a Host RBP Network that Orchestrates Viral RNA SynthesisbioRxivDecember 11, 2025Stephen Clarke, Deep B. Patel, Andrea Ascura, Caroline L. Wiser, Jennifer L. Pennise, Samantha M. Lisy, Manuel AscanoUniversity),N/A T4 DNA ligase,New England Biolabs,M0202T RNA 5’Pyrophosphohydrolase (RppH),New England Biolabs,M0356S "RNase inhibitor, Murine",New England Biolabs,M0314S DNase I (RNase free),New England Biolabs,M0303S TPCK-trypsin,Thermo Fischer Scientific

(24) Argonaute-siRNA loading via the RNA-binding protein RDE-4 in C. elegansCurrent BiologyDecember 1, 2025Thiago L. Knittel, Brooke E. Montgomery, Reese A. Sprister, Colin N. Magelky, Margaret J. Smith, Maritza Soto-Ojeda, Melissa Guthrie, Carolyn M. Phillips, Taiowa A. Montgomeryby Thermo Fisher Scientific",Cat# NW04127BOX RNA 5′ Pyrophosphohydrolase,New England Biolabs,Cat# M0356S Alt-R™ S.p. Cas9 Nuclease V3,Integrated DNA Technologies,Cat# 1081058 Critical commercial assays,Critical

(25) TAF1 is required for fetal but not adult hematopoiesis in miceDevelopmental CellNovember 3, 2025Fan Liu, Jingyin Yue, Francesco Tamiro, Jun Sun, Pradeep Kumar Reddy Cingaram, Krystal Lisa Hossack, Concepcion Martinez Caja, Ye Xu, Chuan Chen, Felipe Beckedorff, et al.purified by streptavidin beads M-280 (Invitrogen, 11205D), and the 5' cap was removed using RppH (NEB, M0356S). T4 polynucleotide kinase (NEB, M0201) was then used to repair the 5' hydroxyl terminus. Following

(26) Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiont WolbachiaMicrob GenomOctober 27, 2025Youseuf Suliman, Zhiru Li, Amit Sinha, Philip D. Dyer, Catherine S. Hartley, Laurence Ettwiller, Alistair C. Darby, Clotilde K. Carlow, Benjamin L. MakepeaceRNA to 2.2 µl of 10X ThermoPol buffer (NEB product number B9004) and 2 µl RppH (NEB product number M0356S), followed by incubation at 37 °C for 1 h. Next, 1 µl of 1 : 10 diluted proteinase K was added to the

(27) Argonautes and small RNAs associated with nematode programmed DNA eliminationbioRxivOctober 27, 2025Maxim V. Zagoskin, Yuanyuan Kang, Richard E. Davis, Jianbin Wang°C). To enable cloning of siRNAs carrying 5′ triphosphates, samples were treated with RppH (NEB, catalog #M0356S) in ThermoPol buffer for 1 h at 37 °C to remove cap and convert 5′ di- and triphosphates to monophosphates

(28) The MLLT3 YEATS domain is a dual reader of histone marks (H3K9/18/27ac/cr) and ncRNA (7SK), linking epigenetic and RNA signaling to regulate hematopoiesisbioRxivSeptember 11, 2025Adam Boulton, Ashish Kabra, Nicholas Achille, Emmalee R. Adelman, Dimitrios G. Anastasakis, Felipe Beckedorff, Pradeepkumar Cingaram, Ying Zhang, Benjamin Leach, Ramin Shiekhattar, et al.purified by streptavidin beads M-280 (Invitrogen, 11205D), and the 5’ cap was removed using RppH (NEB, M0356S). T4 polynucleotide kinase (NEB, M0201) was then used to repair the 5’ hydroxyl terminus. Following

(29) Integrator promotes the association of TFIID and RNA polymerase II to maintain pluripotency during developmentMolecular CellAugust 7, 2025Raghu Ram Edupuganti, Felipe Beckedorff, Pradeep Kumar Reddy Cingaram, Saeid Mohammad Parast, Jingyin Yue, Gary LeRoy, Monica Guiselle Valencia, Sadat Dokaneheifard, Helena Gomes Gomes Dos Santos, Omar Lopez Ocejo, et al.Gold,InvivoGen,ant-hg-1 Lipofectamine 3000,Invitrogen,100022052 Biotin-11-NTPs,PerkinElmer,NEL54(2/3/4/5)001 RppH,NEB,M0356S PNK,NEB,M0201L SuperScript III,Invitrogen,18080051 Salmon Sperm DNA,Thermo Scientific,15632011 TSKgel-DEAE-5PW,TOSO

(30) Biochemical analysis of human eIF4E-DCP2 interaction: Implications for the relationship between translation initiation and decappingPLoS OneAugust 1, 2025Zachary F. Mandell, Jeff Collerincubating the 5’ triphosphate RNA oligos with the bacterial RNA 5’ pyrophosphohydrolase, RppH (NEB cat # M0356S), then purified using silica-membrane spin columns. Each of the RNA oligos was of the sequence 5’-AGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUAAGAGC-3’. Recombinant

(31) Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposonsNat CommunJuly 2, 2025KeHuan K. Edmonds, Max E. Wilkinson, Daniel Strebinger, Hongyu Chen, Blake Lash, Clarissa C. Schaefer, Shiyou Zhu, Dangliang Liu, Shai Zilberzwige-Tal, Alim Ladha, et al.RP-HPLC. For 5’ modification, in vitro transcribed RNA (uncapped) were first digested with RppH (NEB, M0356S) to yield 5P-mRNA, which were then ligated to chemically synthesized (and capped) oligonucleotides with

(32) Chemical and topological design of multicapped mRNA and capped circular RNA to augment translationNature BiotechnologyJuly 1, 2025Hongyu Chen, Dangliang Liu, Abhishek Aditham, Jianting Guo, Jiahao Huang, Franklin Kostas, Kamal Maher, Mirco J. Friedrich, Ramnik J. Xavier, Feng Zhang, et al.oligonucleotides to mRNAs The 5′-triphosphorylated mRNA generated by IVT was first treated with RppH (NEB, M0356S) according to the manufacturer’s protocol to generate 5P-mRNA and purified with the Monarch RNA cleanup

(33) tRNA-derived RNA processing in sperm transmits non-genetically inherited phenotypes to offspring in C. elegansResearchSquareJune 6, 2025Nicholas S. Galambos, Olivia J. Galambos, Blair K. Schneider, Kennelly S. Schneider, Katrin E. Allerton, Alexandra E. Schneider, Alexandra Schneider, Jared Yukselen, Alper Kucukural, Colin C. Kucukuralafter complete ethanol removal, the pellet was reconstituted in RNA 5´ Pyrophosphohydrolase (RppH - NEB M0356S) per manufacturers guidelines and incubated at 37°C for one hour. Following RppH treatment, small RNAs

(34) Nucleoporins shape germ granule architecture and balance small RNA silencing pathwaysNat CommunMay 8, 2025Kun Shi, Ying Zhang, Zhenzhen Du, Symonne C. Liu, Ivan Leon, Xinyu Fan, Heng-Chi Lee, Donglei Zhangin 15 μL of nuclease-free water. The RNA samples underwent treatment with RppH (New England Biolabs, M0356S) to convert 22G-RNA 5′ triphosphates to monophosphates. RNA was then concentrated using the RNA Clean

(35) Decoding m6Am by simultaneous transcription-start mapping and methylation quantificationElifeMarch 31, 2025Jianheng Fox Liu, Ben R Hawley, Luke S Nicholson, Samie R Jaffreyeluate was cleaned again by ethanol-AMPure XP. The DTB-GTP cap was removed using 0.5 U/µl RppH (NEB #M0356S) in 1 X ThermoPol buffer (NEB #M0356S) and incubating at 37 °C for 1 hr. The resulting 5’-monophosphate

(36) Decoding m6Am by simultaneous transcription-start mapping and methylation quantificationElifeMarch 31, 2025Jianheng Fox Liu, Ben R Hawley, Luke S Nicholson, Samie R Jaffreyeluate was cleaned again by ethanol-AMPure XP. The DTB-GTP cap was removed using 0.5 U/µl RppH (NEB #M0356S) in 1 X ThermoPol buffer (NEB #M0356S) and incubating at 37 °C for 1 hr. The resulting 5’-monophosphate

(37) Mouse promoters are characterised by low occupancy and high turnover of RNA polymerase IIMolecular Systems BiologyMarch 31, 2025Kasit Chatsirisupachai, Christina J I Moene, Rozemarijn Kleinendorst, Elisa Kreibich, Nacho Molina, Arnaud Krebs,New England Biolabs,M0204L EGTA,Sigma-Aldrich,E3889 RNA 5’ Pyrophosphohydrolase (RppH) - 200 units,New England Biolabs,M0356S Superase-In RNase Inhibitor,Thermo Fisher, Q5 High-Fidelity DNA Polymerase—100 units,New England Biolabs,M0491S Sarkosyl,Sigma-Aldrich,61739 SYBR

(38) Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cellsCell DiscovMarch 18, 2025Lili Li, Xiaoyin Tang, Xuanxuan Guo, Di Rao, Lin Zeng, Junchao Xue, Shuxian Liu, Shikui Tu, En-Zhi Shenprocedure was performed as described previously98. Briefly, RNA 5′ Pyrophosphohydrolase (RppH) (NEB, M0356S) was used to trim the 5′ triphosphate of endogenous 22G-RNAs to monophosphate. The 3′ and 5′ adapters

(39) The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expressionJournal of VirologyFebruary 25, 2025Ruth Verhamme, Robert J. J. Jansens, Jianheng Liu, Fien Van Raemdonck, Cliff Van Waesberghe, Luke Nicholson, Samie R. Jaffrey, Herman W. Favoreelenrichment, the RNA fragments were eluted into water. To ligate the 5′ adapter, we used RppH (NEB #M0356S) to release the 3′-Desthiobiotin caps, which results in monophosphate 5′ ends ready for ligation. We

(40) Decreased SynMuv B gene activity in response to viral infection leads to activation of the antiviral RNAi pathway in C. elegansPLoS BiolJanuary 29, 2025Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H. X. Liu, Jennifer D. Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J. Tate, Taiowa A. Montgomery, Gary Ruvkuntriphosphates to monophosphates following the manufacturer’s recommendations (New England Biolabs, cat# M0356S) [77]; 16 to 30-nt small RNAs were size selected on a 17% polyacrylamide/urea gel and purified using

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RNA 5 Pyrophosphohydrolase (RppH)

RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

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RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

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