RNA 5 Pyrophosphohydrolase (RppH) M0356S from New England Biolabs

Product Specs
ItemRNA 5 Pyrophosphohydrolase (RppH)
CompanyNew England Biolabs
Price $103.00 Inquire
Catalog NumberM0356S
Size200 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(61) A-MYB and BRDT-dependent RNA Polymerase II pause release orchestrates transcriptional regulation in mammalian meiosisNature CommunicationsMarch 29, 2023Adriana K. Alexander, Edward J. Rice, Jelena Lujic, Leah E. Simon, Stephanie Tanis, Gilad Barshad, Lina Zhu, Jyoti Lama, Paula E. Cohen, Charles G. DankoM0204L). After this, a second bead binding was performed followed by a 5′ de-capping using RppH (NEB M0356S). The 5′ end of the transcript was phosphorylated using PNK (NEB M0201L) and then purified with Trizol.

(62) CoLoC-seq probes the global topology of organelle transcriptomesNucleic acids researchFebruary 22, 2023Jeandard D, Smirnova A, Fasemore AM, Coudray L, Entelis N, Förstner KU, Tarassov I, Smirnov A.(Thermo Fisher Scientific, Waltham, USA, Cat # 88216), RNA 5’-pyrophosphohydrolase (New England Biolabs, Ipswich, USA, Cat # M0356S), RNase A (Thermo Fisher Scientific, Waltham, USA, Cat # EN0531), RNase A/T1 mix (2 mg/ml, 5,000 U/ml;

(63) PRO-IP-seq Tracks Molecular Modifications of Engaged Pol II Complexes at Nucleotide ResolutionbioRxivFebruary 6, 2023Anniina Vihervaara, Philip Versluis, John T. Lisconducted on-beads. The bead-nascent-RNA-complexes were diluted in RNA 5' Pyrophosphorylase mix (NEB, M0356S) and incubated at 37 °C for 45 min. The 5'-hydroxyrepair was subsequently conducted by adding T4 polynucleotide kinase

(64) Profiling lariat intermediates reveals genetic determinants of early and late co-transcriptional splicingMolecular cellDecember 15, 2022Zeng Y, Fair BJ, Zeng H, Krishnamohan A, Hou Y, Hall JM, Ruthenburg AJ, Li YI, Staley JP.,Thermo Fisher Scientific,AM9344 Critical Commercial Assays,, Turbo DNase,Thermo Fisher Scientific,AM2238 RppH,NEB,M0356S Terminator™ 5´-Phosphate-Dependent Exonuclease,Lucigen,TER51020 T4 RNA Ligase 2 truncated K227Q,NEB,M0351L Superscript

(65) Comprehensive microRNA analysis across genome-edited colorectal cancer organoid models reveals miR-24 as a candidate regulator of cell survivalBMC genomicsDecember 1, 2022Villanueva JW, Kwong L, Han T, Martinez SA, Shanahan MT, Kanke M, Dow LE, Danko CG, Sethupathy P.(i) Removal of the 5’ cap using RNA 5’ pyrophosphohydrolase (RppH, New England Biolabs, Ipswich, MA, M0356S) (ii) Phosphorylation of the 5’ end using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA,

(66) The methyl phosphate capping enzyme Bmc1/Bin3 is a stable component of the fission yeast telomerase holoenzymeNature CommunicationsDecember 1, 2022Jennifer Porat, Moaine El Baidouri, Jorg Grigull, Jean-Marc Deragon, Mark A. Bayfieldphosphatase (NEB, M0290), followed by decapping and circularization with RNA 5’ Pyrophosphohydrolase (NEB, M0356S) and T4 RNA Ligase 1 (NEB, M0204S). TER1 was amplified from circularized RNA with the OneStep RT-PCR Kit

(67) Polyadenylation ligation‐mediated sequencing (PALM‐Seq) characterizes cell‐free coding and non‐coding RNAs in human biofluidsClinical and Translational MedicineJuly 20, 2022Zhongzhen Liu et al.preparation could be added. 68 , 69 We have tried to de‐cap the input cfRNAs using RppH (NEB, cat. M0356S) before constructing PALM‐Seq libraries. Our data showed no significant differences with or without

(68) Genetic dissection of the RNA polymerase II transcription cycleElifeJuly 1, 2022Shao-Pei Chou, Adriana K Alexander, Edward J Rice, Lauren A Choate, Charles G DankoLigase 1 (NEB, M0204L). We performed a second bead binding followed by a 5′ decapping with RppH (NEB, M0356S). RNA was phosphorylated on the 5’ end using T4 polynucleotide kinase (NEB, M0201L) then ligated onto

(69) Genetic dissection of the RNA polymerase II transcription cycleeLifeJuly 1, 2022Shao-Pei Chou, Adriana K Alexander, Edward J Rice, Lauren A Choate, Charles G DankoLigase 1 (NEB, M0204L). We performed a second bead binding followed by a 5′ decapping with RppH (NEB, M0356S). RNA was phosphorylated on the 5’ end using T4 polynucleotide kinase (NEB, M0201L) then ligated onto

(70) Dynamic and widespread control of poly(A) tail length during macrophage activationRNAJuly 1, 2022Yeonui Kwak, Ciarán W.P. Daly, Elizabeth A. Fogarty, Andrew Grimson, Hojoong Kwakwas performed (NEB; M0204L), followed by another round of bead binding, 5′ decapping using RppH (NEB, M0356S), 5′ end phosphorylation (NEB, M0201S), and then 5′ adaptor ligation. After the 5′ adaptor ligation,

(71) PABLO-QA: A sensitive assay for quantifying monophosphorylated RNA 5′ endsSTAR ProtocolsJune 1, 2022Jamie Richards, Joel G. Belascorecombinant proteins","Chemicals, peptides, and recombinant proteins" ,, RppH,New England Biolabs,M0356S 10× RppH buffer,New England Biolabs,M0356S Phusion high-fidelity DNA polymerase,Thermo Fisher Scientific,F530S 5×

(72) m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translationCell discoveryMay 21, 2022Zhuo W, Sun M, Wang K, Zhang L, Li K, Yi D, Li M, Sun Q, Ma X, Liu W, et al.by LC-MS/MS analysis One hundred and fifty nanograms isolated RNA was decapped with 10 U RppH (NEB, M0356S) in ThermoPol buffer at 37 °C for 3 h. The decapped RNA was purified by ethanol precipitation, and then

(73) TFIID Enables RNA Polymerase II Promoter-Proximal PausingSSRN Electronic JournalMarch 31, 2022Charli Fant et al.TRIzol™ LS Reagent Invitrogen cat#10296028 T4 Polynucleotide Kinase NEB cat#M0201S RNA 5' Pyrophosphohydrolase (RppH) NEB cat#M0356S ThermoPol® Reaction Buffer Pack NEB cat#B9004S T4 RNA Ligase 1 (ssRNA Ligase) NEB cat#M0204S SuperScript™ III Reverse Transcriptase

(74) Membrane-associated cytoplasmic granules carrying the Argonaute protein WAGO-3 enable paternal epigenetic inheritance in Caenorhabditis elegansNature cell biologyFebruary 1, 2022Schreier J, Dietz S, Boermel M, Oorschot V, Seistrup AS, de Jesus Domingues AM, Bronkhorst AW, Nguyen DAH, Phillis S, Gleason EJ, et al.Qubit™ RNA BR Assay Kit (Art. No. Q10210, Invitrogen™), respectively. RNA 5' Pyrophosphohydrolase (RppH) (Art. No. M0356S, New England BioLabs®) treatment was performed with a starting amount of 690 ng. After purification

(75) Dual roles for piRNAs in promoting and preventing gene silencing in C. elegansCell ReportsDecember 7, 2021Brooke E. Montgomery, Tarah Vijayasarathy, Taylor N. Marks, Charlotte A. Cialek, Kailee J. Reed, Taiowa A. MontgomeryTranscription Kit,Thermo Fisher Scientific,Cat# 4366596 RNA 5′ Pyrophosphohydrolase,New England Biolabs,Cat# M0356S "Acrylamide/Bis 19:1, 40% (w/v) solution",Thermo Fisher Scientific,Cat# AM9022 AMPure XP Beads,Beckman Coulter

(76) m6Am-seq reveals the dynamic m6Am methylation in the human transcriptomeNature CommunicationsDecember 1, 2021Hanxiao Sun, Kai Li, Xiaoting Zhang, Jun’e Liu, Meiling Zhang, Haowei Meng, Chengqi YiAM1908). Quantification of m6A and m6Am by LC–MS/MS For m6Am, 150 ng RNA was decapped by 10 units of RppH (NEB, M0356S) in Thermopol buffer for 5 h at 37 °C. RNA was purified by RNA clean & concentrator kit (Zymo Research,

(77) TERA-Seq: true end-to-end sequencing of native RNA molecules for transcriptome characterizationNucleic Acids ResearchNovember 18, 2021Fadia Ibrahim, Jan Oppelt, Manolis Maragkakis, Zissimos MourelatosLigase I (NEB, M0204S)",Y,Y,Y "RtcB (NEB, M0458S)",Y,, "Quick CIP (NEB, M0525S)",Y,, "RppH (NEB, M0356S)",Y,,Y "10x Thermopol buffer (NEB, B9004S)",Y,,Y "Quick Ligation Reaction Buffer (NEB, B6058)",Y,Y,Y "T4 DNA Ligase

(78) Deep (Meta)genomics and (Meta)transcriptome Analyses of Fungal and Bacteria Consortia From Aircraft Tanks and Kerosene Identify Key Genes in Fuel and Tank CorrosionFrontiers in MicrobiologyOctober 1, 2021Ines Krohn et al.phosphorylated at the 5′ end, and decapped using 10 U T4-PNK ± 40 nmol ATP and 5 U RppH, respectively (M0356S, New England Biolabs, Frankfurt am Main, Germany). After each enzymatic treatment RNA was purified with

(79) Systematic detection of m6A-modified transcripts at single-molecule and single-cell resolutionCell Reports MethodsSeptember 27, 2021Kyung Lock Kim, Peter van Galen, Volker Hovestadt, Gilbert J. Rahme, Ekaterina N. Andreishcheva, Abhijeet Shinde, Elizabeth Gaskell, Daniel R. Jones, Efrat Shema, Bradley E. Bernsteincap-adjacent m6Am in 2x polyA+ RNA, 400 ng of 2x polyA+ RNA was decapped using 25 Units of RppH (NEB, Cat. #M0356S) in 1X ThermoPol buffer (NEB, Cat. #B9004S) for 3 hours at 37°C, followed by clean up with Zymo RNA

(80) DNA-guided photoactivatable probe-based chemical proteomics reveals the reader protein of mRNA methylationiScienceSeptember 24, 2021Yepei Huang, Xue Bai, Zhenchang Guo, Hanyang Dong, Yun Fu, Hui Zhang, Guijin Zhai, Shanshan Tian, Ye Wang, Kai ZhangThermo) were defined as “input-mRNA”, additionally, input-mRNA decapped by RNA 5' Pyrophosphohydrolase (M0356S, NEB) were defined as “input-decapped mRNA”. The extracted RNAs were analyzed by UPLC-MS/MS. UPLC-MS/MS analysis

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RNA 5 Pyrophosphohydrolase (RppH)

RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

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RNA 5 Pyrophosphohydrolase (RppH) from New England Biolabs

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