7-AAD Viability Staining Solution from BioLegend

Product Specs
Item7-AAD Viability Staining Solution
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420404
ApplicationsFC
Quantity500T
FormatPBS, pH 7.2 with 0.09% (w/v) sodium azide
Target7-AAD
Concentration50.0000µg/mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Underlying MASLD-induced gut microbiome dysbiosis and intestinal inflammation are key to poor outcomes in vibriosis infections in a preclinical modelGut MicrobesDecember 31, 2026Punnag Saha, Subhajit Roy, Madhura More, Dipro Bose, Ayushi Trivedi, Bryan W. Brooks, Wing-Kin Syn, Anna Mae Diehl, Saurabh Chatterjeewere stained for viability by incubation with live/dead fixable near-IR viability dye (Catalog No. 420403, BioLegend, San Diego, CA, USA) for 20 min on ice in the dark. Following two washes, fixation was performed

(2) TNFR1 expression on cancer cells mediates immune escape and an immunosuppressive microenvironmentJ Exp MedOctober 5, 2026Elixabet Bolaños, Lorena Cañas-Zabala, Irene Olivera, David Ruiz Guillamón, Enric Vercher, Gabriel Gomis, Carlos E. de Andrea, Raluca Alexandru, Arantza Azpilikueta, Nekane Soria, et al.RRID:AB_326593). The Zombie NIR Fixable viability kit (423106; BioLegend; RRID:SCR_023773) or 7AAD Viability Kit (420404; BioLegend; RRID:SCR_023774) was used as a live/dead cell marker. All flow cytometry stainings were

(3) Intracellular Staphylococcus aureus triggers isolate-specific host transcriptional responses alongside TNF-R1 regulated cell fate decisionsCell Commun SignalSeptember 28, 2026Annika Walter, Thorsten Bischler, Marvin Jungblut, Leonhard F. Breitsprecher, Filip Vilotic, Johanna Beck, Lucia Hofmann, Nicole Schäfer, Tanja Ziesmann, Rico Franzkoch, et al.FAM-YVAD-FMK substrate (#13483, Biomol) was diluted 1:250 in 250 µL cell suspension with 5 µL 7-AAD (#420404, BioLegend), followed by 1 h incubation under cell culture conditions. Cell suspensions were washed

(4) Hippuric acid contributes to NK-cell activation with dependence on TLR2 in chronic hepatitis B complicated by MASLDiScienceSeptember 18, 2026Luyuan Zhang, Anqi Li, Ke Xu, Siyang Tao, Meijuan ZhengBS099-500g Mannitol,MCE,Cat# HY-N0378 Monensin,MultiSciences Biotech,Cat# CS0004 7-AAD,BioLegend,Cat# 420403 Recombinant human IL-12,PeproTech,Cat# 200-12 Recombinant human IL-15,PeproTech,Cat# 200-15 ,, Deposited

(5) Single-cell transcriptomics reveals critical role of CD74 in bone marrow-derived mesenchymal stem cellsStem Cells Transl MedSeptember 18, 2026Qinqin Xu, Carson Curtis, Jiahao Zhang, Arjun Verma, Aaron J Sheppard, Jeffrey Deng, Hicham Drissi, Shane Barton, Patrick Massey, Yufeng DongCell Staining Buffer, and 5 µL (0.25 µg/million cells) of 7-AAD Viability Staining Solution (BioLegend #420403) was added to exclude dead cells. After incubation on ice for 3-5 min in the dark, samples were analyzed

(6) Antimetabolites synergize with antibody drug conjugate-based non-genotoxic conditioning facilitating hematopoietic stem cell-directed lentiviral gene therapyMolecular Therapy AdvancesSeptember 10, 2026Jennifer Okalova, Jordan S. Alexander, Seema R. Patel, Gianna M. Branella, Carlos Barichello de Quevedo, W. Hunter Baldwin, Chengyu Prince, Shanmuganathan Chandrakasan, Harrison C. Brown, Christopher B. Doering, et al.analyzer. Dead cells were excluded from the analysis using 3 μL of 7-AAD Viability Dye (BioLegend, Cat #420404) that was added 2 min before the sample was run. For the cycling analyses, femurs were processed as

(7) Exploring the functions of NKG7 in CD4+ and CD8+ T cells during malariaPLoS PathogSeptember 8, 2026Teija C-M. Frame, Fabian de Labastida Rivera, Nicholas L. Dooley, Jessica A. Engel, Jinrui Na, Yulin Wang, Luzia Bukali, Susanna S. Ng, Patrick T. Bunn, Lynette Beattie, et al.,BD Biosciences,564616,,,, Annexin V,"Apoptosis, survival",BD Biosciences,559763,,,, 7-AAD,"Apoptosis, survival",BioLegend,420404,,,, Phenotypic flow cytometry markers for apoptosis, activation and proliferation markers (S2 Fig).

(8) Paeoniflorin prevents acute graft-versus-host disease while preserving graft-versus-tumor effectsFront ImmunolSeptember 8, 2026Xingyu Wan, Nan Li, Yang Gou, Wuchen Yang, Shengwang Wu, Jing Zhang, Shuiqing Liu, Xi Zhangcell apoptosis was evaluated using Annexin V-PE (#C1065L, Beyotime) and 7-aminoactinomycin D (7-AAD) (#420404, BioLegend) according to manufacturer’s instructions. Transcriptome sequencing Splenic donor-derived

(9) Recruited monocyte-derived macrophages drive T cell inflammation in immune checkpoint inhibitor-mediated pneumonitisCancer CommunicationsSeptember 2, 2026Xiaoran Cui, Renyong Zhi, Xiaoyan Li, Yinming Ji, Zhisong Hu, Pengfei Cui, Yuhui Qin, Tao Li, Liangliang Wu, Lingxiong Wang, et al.500 μl of Cell Staining Buffer and stained with 5 μl of 7-aminoactinomycin D (7-AAD) Viability Dye (#420403, BioLegend) for 5 min. 7-AAD-negative cells were isolated via flow cytometry sorting and collected in

(10) LIM and SH3 protein 1 deficiency confers resistance to cytotoxic lymphocyte-mediated lysis and immunotherapy in gastric cancer by disrupting cytoskeleton dynamicsJ Immunother CancerSeptember 2, 2026Teming Zhang, Yingying Sun, Chao Chen, Jingwei Zheng, Liyun Chang, Yizhen Xiang, Qiongying Zhang, Haoliang Li, Wangkai Xie, Qiaoyi Xu, et al.BioLegend), PD-1 (329906, BioLegend), LAG-3 (369330, BioLegend), TIM-3 (17–3109-42, eBioscience), and 7-AAD (420404, BioLegend). On the third day, following a 4-hour treatment of cells with Brefeldin A (420601, BioLegend),

(11) Dual Targeting of FGFR4 and PI3K-mTOR Suppresses Tumor-Associated Phenotypes in Pancreatic Ductal AdenocarcinomaInt J Mol SciAugust 28, 2026Joseph A. Goode, Savannah A. Harris, Deborah A. Altomarewith Annexin V-Pacific Blue (BioLegend, San Diego, CA, USA, cat. #640918) and 7-AAD (BioLegend, cat. #420404) for 20 min at RT. Cells were washed and flow cytometry was performed using a CytoFlex LX (Beckman Coulter,

(12) The Comprehensive Live Cell-Based Cytotoxicity Assay for Monitoring Disease Activity and Guiding Rescue Therapy in Acute Attacks of NMOSDAnn Clin Transl NeurolAugust 26, 2026Xiaona Xu, Jie Ding, Yupeng Du, Guibao Luo, Jinming Li, Rui Liu, Xiaoyi Xu, Xiao Li, Minshu Li, Wei Jiang, et al.non‐viable cell populations, cell suspensions were stained with 5 μL of 7‐aminoactinomycin D (7‐AAD; Cat#420404; BioLegend, San Diego, CA, USA) and incubated for 10 min at room temperature in the dark. The endogenous

(13) Comparative In Vitro Apoptotic Activity of Two Chimeric Anti-CD99 Antibodies Recognizing Distinct CD99 Regions in T-ALL ModelsBiomedicinesAugust 26, 2026Phasinee Juengsamretkarn, Phakhwan Sampaoloi, Kadkanok Ruangkul, Manatchanok Chinakarapong, Praweekorn Pliensak, Myint Myat Thu, Tawan Chokepaichitkool, Supansa Pata, Witida Laopajon, Watchara Kasinrerk, et al.Diego, CA, USA, #640945, lot: B404229 and B480747) and 7-AAD (0.4 µg/mL; BioLegend, San Diego, CA, USA, #420404, lot: B402850). to assess apoptosis. The percentage of apoptotic cells was analyzed using an Accuri

(14) Shared Treg dysregulation underlies psoriasis vulgaris and atopic dermatitis through the PHF19-PRC2 epigenetic axisFrontiers in ImmunologyAugust 20, 2026Yiting Lin, Xiaolei Su, Mingzhu Jin, Yuqi Wang, Yaoyao Li, Bingquan Zong, Haoran Song, Yajie Lv, Zhenhua Liu, Gang Wang, et al.120119), anti-GITR (clone DTA-1, BioLegend, Cat. No. 126311), and 7AAD viability dye (BioLegend, Cat. No. 420404). After washing with PBS, cells were fixed and permeabilized using the eBioscience™ Foxp3/Transcription

(15) Genetic Engineering of Tumor-Infiltrating Lymphocytes (TIL) via a T-Editor Platform to Enhance Anti-Tumor ActivityCancer SciAugust 19, 2026Fenge Li, Xuejiao Ma, Yongming Xue, Jilong Yang, Ruijing Wang, Xiang Li, Chao Zhang, Wenhan Lu, Mei Liu, Yue Xu, et al.analysis, cells were harvested and stained with 1 μg/mL 7‐amino‐actinomycin D (7‐AAD; BioLegend, Cat. No. 420403) for 20 min at 4°C in the dark, then analyzed by flow cytometry. The percentage of 7‐AAD‐positive cells

(16) T cell receptor repertoire dynamics and newly identified functional regulators of autologous tumor-infiltrating lymphocyte therapy in advanced solid tumorsJ Transl MedAugust 11, 2026Fenge Li, Jilong Yang, Gregory Lizee, Yongming Xue, Ruijing Wang, Chao Zhang, Xinbo Gao, Xiangqin Zhao, Mei Liu, Wenhan Lu, et al.before analysis, cells were harvested and stained with 1 µg/mL 7-aminoactinomycin D (7-AAD; BioLegend, 420403) for 20 min at 4 °C in the dark, followed by flow cytometric analysis. The percentage of 7-AAD⁺ cells

(17) Salinomycin is effective for clinically relevant radioresistant oral squamous cell carcinoma cancer cells and their cancer stem cell marker positive fractionMedicine InternationalAugust 11, 2026Ryo Saga, Yoichiro Hosokawa, Manabu Fukumotowere detected using FITC-conjugated Annexin V (cat. no. 640906, BioLegend, Inc.) and 7-AAD (cat. no. 420404, BioLegend, Inc.). Trypsinized cells were adjusted to a density of 1x106 cells/ml and washed with PBS(-);

(18) Pathophysiology of the gut–eye axis in a Sjögren's disease‐like dry eye mouse model: An exploratory evaluation of dietary heat‐treated Enterococcus faecalis FK‐23Exp PhysiolAugust 10, 2026Kenji Goto, Shigekazu Takemura, Yukiko Minamiyama, Toshikazu Yoshikawaflow cytometry analysis, the cells were incubated with 5 µL of 7‐amino‐actinomycin D (RRID: N/A; catalogue no. 420403; BioLegend) for 5 min on ice, then quantified by flow cytometry analysis using X‐20 (Becton Dickinson

(19) RAS P21 Protein Activator 3 finetunes Type I immunity by limiting HCK-mediated STAT4 phosphorylationNature CommunicationsAugust 5, 2026Jiayu Song, Xiaoyu Liu, Jie Sun, Xi Cheng, Chuan He, Yunhong Zhong, Jinfang Zhang, Junjie Zhang, Hui Xiong, Bing WuCat: 110706, Biolegend), anti-BrdU-FITC (3D4, Cat: 364103, Biolegend), 7-amino-actinomycin D (Cat: 420404, Biolegend), Annexin V (Cat:640907, Biolegend) and anti-Ki-67 (11F6, Cat:151209, Biolegend) were used.

(20) Restricting efferocytosis pathway to CD91 via phosphatidylserine-targeting chimeric protein augments antitumor immune responsesMolecular TherapyAugust 5, 2026Yu Mizote, Hiroki Nishita, Kazuki Hashiba, Chisa Okuma, Sachiko Akane, Kenjiro Minomi, Hideaki Tahara421 (BV421) annexin V (640923, BioLegend, San Diego, CA), and dead cells were costained with 7-AAD (420403, BioLegend) or Helix NP NIR (425301, BioLegend). As a method to detect PS instead of annexin V, the

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(1)

4/17/2023Easy to Use Viability Staining Solution for Live and Dead Cell Detection Soheil Tavakolpour7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.
(1)

3/24/2023Very Good 7AAD Viability Staining for Sorting Im Hong SunOur lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout ... Read Review
(1)

7/7/2022Easy to Use 7AAD Viability Stain Matthew RatsepI use this reagent to identify live/dead cells in flow cytometry experiments. The reagent is easy to use and gives good results. The only issue is that the emission spectrum is right in the middle of ... Read Review
(1)

3/13/2019Staining Human T Cells with 7-AAD Adam GuessThis reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ... Read Review
(1)

8/3/20187AAD Works Well to Determine Viability by Flow Cytometry Suzanne TomchuckIn order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD ... Read Review
(1)

10/29/2015Cell Viability Stain Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

7-AAD Viability Staining Solution from BioLegend

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7-AAD Viability Staining Solution from BioLegend

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