7-AAD Viability Staining Solution from BioLegend

Product Specs
Item7-AAD Viability Staining Solution
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420404
Quantity500 tests
ApplicationsFC - Quality tested
FormatPBS, pH 7.2 with 0.09% (w/v) sodium azide
Target7-AAD
Concentration50µg/ml

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Underlying MASLD-induced gut microbiome dysbiosis and intestinal inflammation are key to poor outcomes in vibriosis infections in a preclinical modelGut MicrobesDecember 31, 2026Punnag Saha, Subhajit Roy, Madhura More, Dipro Bose, Ayushi Trivedi, Bryan W. Brooks, Wing-Kin Syn, Anna Mae Diehl, Saurabh Chatterjeewere stained for viability by incubation with live/dead fixable near-IR viability dye (Catalog No. 420403, BioLegend, San Diego, CA, USA) for 20 min on ice in the dark. Following two washes, fixation was performed

(2) Efficacy and immunogenic effects of Tumor Treating Fields ( TTFields ) in preclinical models of pancreatic ductal adenocarcinoma, with and without gemcitabine/nab‐paclitaxelInternational Journal of CancerJune 15, 2026Tal Kan, Tharwat Haj Khalil, Yiftah Barsheshet, Tali Voloshin, Lilach Koren, Bella Koltun, Cfir David, Kerem Wainer‐Katsir, Avital Vorontsova, Boris Brant, et al.(FITC)‐conjugated Annexin V (AnnV) and 7‐Aminoactinomycin D (7‐AAD) as per manufacturer's instructions (640945 and 420404, BioLegend). Cells were acquired by flow cytometry, and percentages of live (AnnV−/7AAD−), apoptotic

(3) 30-hydroxygambogic acid activates overlapping and independent apoptotic pathways in HPV positive and HPV negative oral cancer cellsTumour Virus ResearchJune 1, 2026Danelle Grubbs, Sonia Whang, Valeria Rodarte, Briza Martinez, Valeri Filippov, John Chen, Jaqueline Coats, Julia Unternaehrer, Isaac Kremsky, Brigitte Vazquez, et al.before following the staining protocol for Annexin V (Biolegend, cat. 640918) and 7AAD (Biolegend, cat. 420403). Each cell line contained ten individual samples: non-stained, single-stained Annexin V, single-stained

(4) Multicellular senescence impairs skeletal muscle recovery following disuse in agingSci AdvMay 29, 2026Paul-Emile Bourrant, Elena M. Yee, Zachary J. Fennel, Robert J. Castro, Chad M. Skiles, Jonathan J. Petrocelli, Naomi M. M. P. de Hart, Lisa A. Lesniewski, Anna E. Beaudin, Katsuhiko Funai, et al.Detection Kit (Dojindo, SG04-01). To improve sorting, 7-aminoactinomycin D (7-AAD) (1:200; BioLegend, 420404) and Hoechst 33342 (1:40,000; Tocris, 5117) were added to exclude debris and to enrich for nucleated

(5) KLF4 overexpression in CAR T cells enhances antitumor potency by preventing exhaustionJ Immunother CancerMay 20, 2026Haoqi Wang, Xianglong Meng, Yuanyuan Feng, Anqi Su, Wentao Qin, Junjie Shen, Kunyu Lu, Lingling Gu, Yujie Ma, Yaxing Wang, et al.plates were incubated at 37°C with 5% CO₂ for 24 hours. After incubation, 7-Aminoactinomycin D (7-AAD, 420404, BioLegend, USA) was added, and the viability of the target cells was measured by flow cytometry. Cytotoxicity

(6) Taxane chemotherapy promotes response to TIM-3 checkpoint blockade via STING-mediated ER stress and HMGB1 secretionCell Rep MedMay 19, 2026Alexis Onimus, Daiana Celias, Shiun Chang, Joshua Davis, Jay Mandula, Jie Li, Alycia Gardner, Kay Hänggi, Olabisi Osunmakinde, Hatem Soliman, et al.50007 Clarity Max™ Western ECL Substrate,Bio-Rad,Cat# 1705062 7-AAD Viability Staining Solution,Biolegend,Cat# 420403 "AMG PERK 44, PERK Inhibitor",MedChemExpress,Cat# HY-12661 A MKC8866 IRE1a RNase Inhibitor,MedChemExpress,Cat#

(7) Tumour-derived SAA1 reprogrammes macrophages to promote CXCL1-mediated metastasis in Ovarian CancerClin Transl MedMay 18, 2026Xuan Zhou, Huangyang Meng, Qianjing Chang, Jingjing Ren, Meichen Wen, Lin Zhang, Wenjun ChengCD86 (BioLegend, 305422), APC–conjugated anti‐human CD206 (BioLegend, 321110), and 7‐AAD (BioLegend, 420404) for 1 h at 4°C. After two washes with flow cytometry buffer, cells were resuspended in 0.5 mL buffer

(8) Age- and tissue-dependent diversity of human plasmacytoid dendritic cells uncovers a cycling subset dominant in early life and cancerImmunityMay 12, 2026Alina Ulezko Antonova, Patrick Fernandes Rodrigues, Chelsea Mannie, Giorgia Ferrari, Horacio Carvajal Dominguez, Mattia Bugatti, William Vermi, Tong Wu, Marina Cella, Pirooz Eghtesady, et al.551811 "Chemicals, peptides, recombinant proteins and others",, 7AAD Viability Staining,Biolegend,Cat# 420404 1M HEPES Buffer,Corning,Cat# 25-060-Cl Beta-Mercaptoethanol,Sigma,Cat# M-7522 Dopamine hydrochloride,Sigma,Cat#

(9) Loss of the autoimmune risk gene TREX1 reveals a convergence of mechanisms promoting immune tolerance loss and antitumor immunitySci AdvMay 8, 2026Junghyun Lim, Katherine Williams, Stephanie Mittman, Patricia Pacheco Sanchez, Ryan Rodriguez, Annie Ogasawara, Grace Barnett, Mayra Cruz Tleugabulova, Barzin Y. Nabet, Jeffrey Hung, et al.Subsequently, cells were labeled with calcein blue (Invitrogen, C1429) and 7-aminoactinomycin D (BioLegend, 420403). Samples were sorted into T cells (CD45+CD90.2+CD11b−NK1.1−CD19−) and non-T immune cells (CD45+CD90.2−CD11b+NK1.1+CD19+)

(10) Optimization of head and neck cancer peripheral blood mononuclear cell processing for robust downstream functional immune analysismedRxivMay 5, 2026Margaret Nelson, Katelyn Jansen, Farah Sagin, Maria Lehn, Hani Alrefai, Claire Girten, Joanna Khoury, Marina Rodriguez, Jacob Garner, Carolyn Schroeder, et al.control and CFSE single stain control, were stained with 7AAD Viability Staining Solution (BioLegend, cat#420403) and immediately acquired on a LSR Fortessa (BD Biosciences, Franklin Lakes, NJ). Live target HN5 cells

(11) CRL4AMBRA1-mediated progesterone receptor degradation drives progestin resistance and represents a therapeutic vulnerability in endometrial cancerInt J Biol SciMay 1, 2026Yaru Sheng, Ting Ni, Xiaoyi Ding, Jiaying Huang, Xueya Zhao, Xinyao Li, Qingting Li, Jing Wang, Xiaoming Yang, Lin Zhang, et al.harvested for staining with Annexin V (BioLegend, #640920) and 7-aminoactinomycin D (7-AAD; BioLegend, #420404) staining. Finally, the cells were analyzed using a BD Fortessa Flow cytometer (BD Biosciences, Franklin

(12) TAF1 Suppresses MHC-I Expression and Correlates with Poor Immunotherapy Response in Small Cell Lung CancerBiomedicinesApril 23, 2026Qing Gao, Kehong Wei, Deshen Pan, Yufei Xi, Chaoliang Xu, Deshui Jiacultured SCLC cell lines, 1–2 × 106 cells were stained with 7 AAD Viability Staining Solution (BioLegend, #420404) together with either APC anti-human HLA A, B, C (BioLegend, #311409; for human lines) or APC anti-mouse H-2Kb/H-2Db

(13) Construction and evaluation of an engineered autonomously lysing Salmonella system coupled with self-assembled nanoparticles as a vaccine platform for antigen deliveryJournal of NanobiotechnologyApril 17, 2026Xiaoping Bian, Limin Jiang, Man Xu, Yue Sun, Xinyu Liu, Tianyu Fan, Mengru Li, Longlong Cao, Qingke Kongbuffer for flow cytometric analysis (FongCyte, Beijing Challen Biotechnology Co., Ltd.). The 7-AAD (420404, BioLegend, USA) was used to exclude dead cells. T cell populations were immunophenotyped, and naïve

(14) Integrative Multi-Omics Analysis Reveals the Immunoregulatory Effects of Sepia Ink on ADHD-like PhenotypesCurr Issues Mol BiolApril 16, 2026Baohong Wei, Jiayi Yin, Wenmin Yuan, Peiling Cai, Qiaoling Song, Zhe Li, Xiaoqing Ma, Xue Yang, Lejia Hong, Huashi Guan, et al.suspensions were incubated with fluorescent anti-mouse antibodies, followed by staining with 7-AAD (Cat. No. 420404, BioLegend, San Diego, CA, USA). Post-incubation, cells were washed with PBS, resuspended, and analyzed

(15) Incubation of brain organoids with fluorescent carbon nanodotsRSC AdvApril 8, 2026Carla Sprengel, Ute Fischer, Arndt Borkhardt, Rainer Haas, Thomas Heinzelwith a 40 µm cell strainer. A concentration of 0.5 µg per ml 7AAD (7-Aminoactinomycin D, BioLegend, #420404) was added prior to the measurement, with an incubation time of at least 5 min in the dark at room temperature,

(16) Peptide Coacervates Promote Cytosolic Delivery of STING Agonists for Cancer ImmunotherapyVaccines (Basel)April 7, 2026Wenlv Zheng, Wei Tang, Jianzheng Wang, Yurong Li, Shengnan Wang, Dan Wu, Xiaoquan Wang, Junmin QuanAlexa Fluor-647 anti-mouse CD3 (100209), FITC-anti-mouse CD8a (100706), anti-mouse CD16/32 (101302) and 7-AAD (420403) were obtained from BioLegend (Beijing, China). Penicillin-Streptomycin (15140122), PE-anti-mouse H-2Kb-SIINFEKL

(17) Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activitybioRxivApril 3, 2026Oriana Y. Teran Pumar, Elton L VanNoy, Abigail Haffey, Durga Prasad Gannamedi, Christine Isabelle Rafie, Dylan Scott Lykke Lykke Harwood, Julia R. Benedetti, Christine Ann Pittman Pittman Ballard, Erika Ciervo, Pedro Henrique Assenza Tavares Assenza Tavares Coroa, et al.hours and then stained at room temperature in the dark with 1:250 viability stain 7-AAD (Biolegend #420404) in Annexin Binding Buffer (Biolegend #79998) for 10 minutes. Cells were washed and resuspended in 150

(18) Biomimetic 3D-Bioprinted organoids of thymic epithelial tumors for translational drug screening and biomarker identificationMaterials Today BioApril 1, 2026Beibei Liu, Huiyan Cheng, Keke Yu, Wen Xu, Xiaoting Tian, Yuhan Xu, Yanbin Kuang, Jun Lu, Rong Li, Xiao Zhang, et al.FC-block (130-091-935,Miltenyi Biotec) to reduce nonspecific staining. Fluorescently labeled 7AAD (420404,Biolegend), CD45 (368512,Biolegend), CD326 (369816,Biolegend),CD8 (344704,Biolegend) and CD3 (317342,Biolegend)

(19) Alcaligenes lipid A as a sublingual adjuvant to augment protective immune responses in the respiratory and gastrointestinal tractsInternational ImmunologyApril 1, 2026Ken Yoshii, Zilai Liu, Atsushi Shimoyama, Yuki Hirayama, Keigo Iemitsu, Eri Node, Koji Hosomi, Hiroshi Kiyono, Koichi Fukase, Jun Kunisawanonspecific staining. Dead cells were detected with 7-aminoactinomycin D (7-AAD; 1:100; catalog no. 420404, BioLegend) and excluded from the analysis. To prevent non-specific fluorochrome interactions when using

(20) EMT activates ER-to-Golgi trafficking through upregulation of REEP2 to promote lung cancer progressionbioRxivMarch 27, 2026Kevin Fulp, Oluwafunminiyi Obaleye, Shike Wang, Xin Liu, Jiang Yu, Jonathan M. Kurie, Jun Xu, William K. Russell, Xiaochao Tan, Guan-Yu Xiaoflow cytometer (BD Symphony). Cell apoptosis assay Cells were harvested and then incubated with 7-AAD (#420404, BioLegend) and Annexin-V (#640926, BioLegend) at room temperature for 10–20 min. After incubation,

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(1)

4/17/2023Easy to Use Viability Staining Solution for Live and Dead Cell Detection Soheil Tavakolpour7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.
(1)

3/24/2023Very Good 7AAD Viability Staining for Sorting Im Hong SunOur lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout ... Read Review
(1)

7/7/2022Easy to Use 7AAD Viability Stain Matthew RatsepI use this reagent to identify live/dead cells in flow cytometry experiments. The reagent is easy to use and gives good results. The only issue is that the emission spectrum is right in the middle of ... Read Review
(1)

3/13/2019Staining Human T Cells with 7-AAD Adam GuessThis reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ... Read Review
(1)

8/3/20187AAD Works Well to Determine Viability by Flow Cytometry Suzanne TomchuckIn order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD ... Read Review
(1)

10/29/2015Cell Viability Stain Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

7-AAD Viability Staining Solution from BioLegend

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7-AAD Viability Staining Solution from BioLegend

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