7-AAD Viability Staining Solution from BioLegend

Product Specs
Item7-AAD Viability Staining Solution
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420404
ApplicationsFC - Quality tested
Quantity500 tests
FormatPBS, pH 7.2 with 0.09% (w/v) sodium azide
Target7-AAD
Concentration50µg/ml

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Underlying MASLD-induced gut microbiome dysbiosis and intestinal inflammation are key to poor outcomes in vibriosis infections in a preclinical modelGut MicrobesDecember 31, 2026Punnag Saha, Subhajit Roy, Madhura More, Dipro Bose, Ayushi Trivedi, Bryan W. Brooks, Wing-Kin Syn, Anna Mae Diehl, Saurabh Chatterjeewere stained for viability by incubation with live/dead fixable near-IR viability dye (Catalog No. 420403, BioLegend, San Diego, CA, USA) for 20 min on ice in the dark. Following two washes, fixation was performed

(2) Efficacy and immunogenic effects of Tumor Treating Fields ( TTFields ) in preclinical models of pancreatic ductal adenocarcinoma, with and without gemcitabine/nab‐paclitaxelInternational Journal of CancerJune 15, 2026Tal Kan, Tharwat Haj Khalil, Yiftah Barsheshet, Tali Voloshin, Lilach Koren, Bella Koltun, Cfir David, Kerem Wainer‐Katsir, Avital Vorontsova, Boris Brant, et al.(FITC)‐conjugated Annexin V (AnnV) and 7‐Aminoactinomycin D (7‐AAD) as per manufacturer's instructions (640945 and 420404, BioLegend). Cells were acquired by flow cytometry, and percentages of live (AnnV−/7AAD−), apoptotic

(3) 30-hydroxygambogic acid activates overlapping and independent apoptotic pathways in HPV positive and HPV negative oral cancer cellsTumour Virus ResearchJune 1, 2026Danelle Grubbs, Sonia Whang, Valeria Rodarte, Briza Martinez, Valeri Filippov, John Chen, Jaqueline Coats, Julia Unternaehrer, Isaac Kremsky, Brigitte Vazquez, et al.before following the staining protocol for Annexin V (Biolegend, cat. 640918) and 7AAD (Biolegend, cat. 420403). Each cell line contained ten individual samples: non-stained, single-stained Annexin V, single-stained

(4) Integrative Multi-Omics Analysis Reveals the Immunoregulatory Effects of Sepia Ink on ADHD-like PhenotypesCurr Issues Mol BiolApril 16, 2026Baohong Wei, Jiayi Yin, Wenmin Yuan, Peiling Cai, Qiaoling Song, Zhe Li, Xiaoqing Ma, Xue Yang, Lejia Hong, Huashi Guan, et al.suspensions were incubated with fluorescent anti-mouse antibodies, followed by staining with 7-AAD (Cat. No. 420404, BioLegend, San Diego, CA, USA). Post-incubation, cells were washed with PBS, resuspended, and analyzed

(5) Incubation of brain organoids with fluorescent carbon nanodotsRSC AdvApril 8, 2026Carla Sprengel, Ute Fischer, Arndt Borkhardt, Rainer Haas, Thomas Heinzelwith a 40 µm cell strainer. A concentration of 0.5 µg per ml 7AAD (7-Aminoactinomycin D, BioLegend, #420404) was added prior to the measurement, with an incubation time of at least 5 min in the dark at room temperature,

(6) Peptide Coacervates Promote Cytosolic Delivery of STING Agonists for Cancer ImmunotherapyVaccines (Basel)April 7, 2026Wenlv Zheng, Wei Tang, Jianzheng Wang, Yurong Li, Shengnan Wang, Dan Wu, Xiaoquan Wang, Junmin QuanAlexa Fluor-647 anti-mouse CD3 (100209), FITC-anti-mouse CD8a (100706), anti-mouse CD16/32 (101302) and 7-AAD (420403) were obtained from BioLegend (Beijing, China). Penicillin-Streptomycin (15140122), PE-anti-mouse H-2Kb-SIINFEKL

(7) Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activitybioRxivApril 3, 2026Oriana Y. Teran Pumar, Elton L VanNoy, Abigail Haffey, Durga Prasad Gannamedi, Christine Isabelle Rafie, Dylan Scott Lykke Lykke Harwood, Julia R. Benedetti, Christine Ann Pittman Pittman Ballard, Erika Ciervo, Pedro Henrique Assenza Tavares Assenza Tavares Coroa, et al.hours and then stained at room temperature in the dark with 1:250 viability stain 7-AAD (Biolegend #420404) in Annexin Binding Buffer (Biolegend #79998) for 10 minutes. Cells were washed and resuspended in 150

(8) Biomimetic 3D-Bioprinted organoids of thymic epithelial tumors for translational drug screening and biomarker identificationMaterials Today BioApril 1, 2026Beibei Liu, Huiyan Cheng, Keke Yu, Wen Xu, Xiaoting Tian, Yuhan Xu, Yanbin Kuang, Jun Lu, Rong Li, Xiao Zhang, et al.FC-block (130-091-935,Miltenyi Biotec) to reduce nonspecific staining. Fluorescently labeled 7AAD (420404,Biolegend), CD45 (368512,Biolegend), CD326 (369816,Biolegend),CD8 (344704,Biolegend) and CD3 (317342,Biolegend)

(9) Alcaligenes lipid A as a sublingual adjuvant to augment protective immune responses in the respiratory and gastrointestinal tractsInternational ImmunologyApril 1, 2026Ken Yoshii, Zilai Liu, Atsushi Shimoyama, Yuki Hirayama, Keigo Iemitsu, Eri Node, Koji Hosomi, Hiroshi Kiyono, Koichi Fukase, Jun Kunisawanonspecific staining. Dead cells were detected with 7-aminoactinomycin D (7-AAD; 1:100; catalog no. 420404, BioLegend) and excluded from the analysis. To prevent non-specific fluorochrome interactions when using

(10) Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programsiScienceMarch 20, 2026Maya R. MacLean, Bianca Laura Bernardoni, Wasundara Fernando, Giovanni Petrarolo, Ilaria D’Agostino, Cheryl A. Dean, Jaganathan Venkatesh, Christopher S. Hughes, Kerry B. Goralski, Geetha Subramanian, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, 7AAD viability dye,BioLegend,Cat#420404 Puromycin,SigmaAldrich,Cat#P8833 TRizol,Invitrogen,Cat#15596018 Matrigel,Corning,Cat#CLS356237 Indomethacin,SigmaAldrich,Cat#I7378 CLM296,This

(11) Engineered Energy-Harvesting Hybrid Nanoscintillators for Enhanced Cancer RadiotherapyACS Appl Mater InterfacesMarch 18, 2026Valeria Secchi, Irene Villa, Samuela Sala, Alessandro Colombo, Stefania Garbujo, Miriam Colombo, Angelo MonguzziA13201) and incubated for 5 min at room temperature in the dark. Subsequently, 5 μL of 7-AAD (BioLegend, 420404) was added, followed by an additional 10 min of incubation in the dark. After staining, 50 μL of binding

(12) CDK4/6 inhibition mitigates chemotherapy-induced expansion of TP53-mutant clonal hematopoiesisNat GenetMarch 11, 2026Irenaeus C. C. Chan, Pu Zhang, Xiangyu Pan, Cynthia Castro, Nina Fox, Alexander M. Lewis, Kenyon Weis, Adriana Cuibus, Steven Tittley, Giulia Petrone, et al.BioLegend) and subsequently stained with FITC–anti-annexin V (1:1,000; 640922, BioLegend) and 7-AAD (420403, BioLegend) in annexin V binding buffer for 15 min at room temperature. The cells were resuspended with

(13) Fine tuning of TCR signaling via CD8αβ and PD-1 and the fate of autoreactive thymocytes during negative selectionImmunohorizonsMarch 10, 2026Martha C Zúñiga, Sangwon Hyun, Jacob Du, Shahar Dubiner, Alicia Freedman-Goretsky, Yitzhar Goretsky, Anita Pothukuchi, Nicholas A Y Fong, Alexander Berg, Caitlin Davis, et al.CD5,100643/25UG,BioLegend PE/DazzleTM 594 anti-mouse CD5,BioLegend,100644/100UG AF488 anti-mouse IgG,A11017,Invitrogen 7-AAD,420403,BioLegend APC Annexin V,640920,BioLegend Biotin anti-mouse CD45.2,109803,BioLegend Biotin anti-mouse CD5

(14) Early Marrow Microenvironment Immune Patterns After Hematopoietic Stem Cell Transplant in Pediatric Acute Lymphoblastic Leukemia Are Associated with Later Development of Chronic GvHD and RelapseInt J Mol SciMarch 2, 2026Catherine M. Njeru, Bernard Ng, Sayeh Abdossamadi, Alima Suleimenova, Carmen Dolores De Luca, Vaishnavi Parthasarathy, Laura M. Sly, Gregor S. D. Reid, Chia Huan Ng, Kirk R. Schultz(eBioscience, San Diego, CA, USA, clone 2D1, Cat # 17-9459-41) and 7-AAD (BioLegend, San Diego, CA, USA, Cat # 420404), and live CD45+ cells were sorted using a BD FACSAria IIu Cell Sorter (BD Biosciences, San Jose, CA,

(15) Viral infection and brain inflammation with seizures in PARK7 deficiencyJournal of Human ImmunityMarch 2, 2026Jonas Lønskov, Annika Sünderhauf, Sisse Andersen, Caroline Bækmann Jeppesen, Franziska Winzig, Daniëla Maria Hinke, Johanna L. Heinz, Kenneth Thomsen, Mette B. Thorup, Thomas Zillinger, et al.and stained for viability with 60 nM SYTOX Green (#S34860; Thermo Fisher Scientific) or 5 μl 7-AAD (#420403; BioLegend) and 2.5 μl Annexin-V-APC (#640941; BioLegend) or Annexin-V-PE (# 640908; BioLegend) in 50

(16) CRISPR/Cas9-mediated B2m knockout paves the way for allogeneic basal cell transplantationRespiratory ResearchFebruary 28, 2026Huanjie Chen, Weizhan Luo, Ning Ma, Shiyue Li(#115008, BioLegend, USA), respectively. Prior to flow cytometry analysis, BCs were stained with 7‐AAD (#420403, Biolegend, USA) to distinguish the live and dead cells. BCs stained with 7‐AAD but not the antibodies

(17) Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosisImmunityFebruary 24, 2026Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins, et al.Purified Annexin V,BioLegend,Cat#640902 10X Annexin V Binding Buffer,BD Bioscience,Cat#556454 7-AAD,Biolegend,Cat#420403 13C6-lysine,Cambridge Isotopes,Cat#201740-81-0 12C6-arginine,Cambridge Isotopes,Cat#1119-34-2 L-Glutamine,Sigma-Aldrich,Cat#G8540-25G RPMI

(18) The Dual Mechanism of Action of CO-005 Overcomes CD20 Resistance in Diffuse Large B-Cell LymphomaImmunoTargets and TherapyFebruary 19, 2026Sittana Matar, Seham Skah, Liza E B Moltu, Øystein Åmellem, Hanne R Hagland, Kjetil Hestdal, Rolf D Pettersen, Nina RichartzμL staining buffer for acquisition. For samples not treated with doxorubicin, 7-AAD (BioLegend, Cat# 420403; final concentration 25 μg/mL) was added for live/dead discrimination. Flow cytometric analysis was

(19) Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytesSignal Transduction and Targeted TherapyFebruary 18, 2026Ziyin Zhang, Nanshu Xiang, Qian Liu, Yanfeng Li, Linhua Wang, Xinpu Yang, Ju Liu, Lichen Zhang, Liaoxun Lu, Yinming Liang, et al.(BioLegend, 640908) was used to detect apoptotic cells; and 7-AAD viability staining solution (BioLegend, 420404) was used to distinguish live and dead cells. To minimize nonspecific binding, the cells were blocked

(20) GPX4 Inhibitor Resistance and Metastatic Features in Triple‐Negative Breast CancerAdvanced ScienceFebruary 17, 2026Marie Sabatier, Mayher Kaur, Milena Chaufan, Felix‐Levin Hormann, Luiza Martins Nascentes Melo, Mario Palma, Jordan Torpey, Yanshan Liang, Alanis Carmona, Cameron Fraser, et al.ice‐cold PBS, and dead cells were stained with 5 µL/106 cells 7‐AAD fluorescent DNA stain (Biolegend, Cat#420404) before flow cytometry analysis. To quantify lipid peroxidation levels in cancer cells, we measured

Write a Review

(1)

4/17/2023Easy to Use Viability Staining Solution for Live and Dead Cell Detection Soheil Tavakolpour7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.
(1)

3/24/2023Very Good 7AAD Viability Staining for Sorting Im Hong SunOur lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout ... Read Review
(1)

7/7/2022Easy to Use 7AAD Viability Stain Matthew RatsepI use this reagent to identify live/dead cells in flow cytometry experiments. The reagent is easy to use and gives good results. The only issue is that the emission spectrum is right in the middle of ... Read Review
(1)

3/13/2019Staining Human T Cells with 7-AAD Adam GuessThis reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ... Read Review
(1)

8/3/20187AAD Works Well to Determine Viability by Flow Cytometry Suzanne TomchuckIn order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD ... Read Review
(1)

10/29/2015Cell Viability Stain Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

7-AAD Viability Staining Solution from BioLegend

Description

BioLegend

Citations (751)

Reviews

7-AAD Viability Staining Solution from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map