7-AAD Viability Staining Solution from BioLegend

Product Specs
Item7-AAD Viability Staining Solution
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420404
ApplicationsFC - Quality tested
Quantity500 tests
FormatPBS, pH 7.2 with 0.09% (w/v) sodium azide
Target7-AAD
Concentration50µg/ml

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Underlying MASLD-induced gut microbiome dysbiosis and intestinal inflammation are key to poor outcomes in vibriosis infections in a preclinical modelGut MicrobesDecember 31, 2026Punnag Saha, Subhajit Roy, Madhura More, Dipro Bose, Ayushi Trivedi, Bryan W. Brooks, Wing-Kin Syn, Anna Mae Diehl, Saurabh Chatterjeewere stained for viability by incubation with live/dead fixable near-IR viability dye (Catalog No. 420403, BioLegend, San Diego, CA, USA) for 20 min on ice in the dark. Following two washes, fixation was performed

(2) Antimetabolites synergize with antibody drug conjugate-based non-genotoxic conditioning facilitating hematopoietic stem cell-directed lentiviral gene therapyMolecular Therapy AdvancesSeptember 10, 2026Jennifer Okalova, Jordan S. Alexander, Seema R. Patel, Gianna M. Branella, Carlos Barichello de Quevedo, W. Hunter Baldwin, Chengyu Prince, Shanmuganathan Chandrakasan, Harrison C. Brown, Christopher B. Doering, et al.analyzer. Dead cells were excluded from the analysis using 3 μL of 7-AAD Viability Dye (BioLegend, Cat #420404) that was added 2 min before the sample was run. For the cycling analyses, femurs were processed as

(3) Liver Endothelia Orchestrate MASH-Associated Macrophage Zonation Through FSTL1-ITGA4 AxisAdv Sci (Weinh)August 3, 2026Lin Sun, Zhensheng Yue, Zhiqiang Fang, Hao Xu, Wei Du, Yuwei Ling, Jingjing Liu, Ping Song, Fei He, Juanli Duan, et al.CD11b‑Pacific Blue (101223, BioLegend), and Ly6C‐FITC (128005, BioLegend). Viability was assessed using 7‑AAD (420404, BioLegend). Flow cytometry was performed on a Sony MA900, and data were analyzed with Cell Sorter software

(4) Adipocyte-derived LTB4 programs human NKG2A+ γδ T cells as cytotoxic sentinels at the adipose-tumor interface in breast cancerJ Immunother CancerJuly 28, 2026Xiaoxiao Hu, Dang Wu, Fang Jia, Sangsang Zhu, Shouyu Ke, Di Chen, Jing Zhao, Lili Li, Yi Zhang, Zhen Wang, et al.FITC anti-human CD3 (BioLegend, Cat# 300406), and 7-AAD Viability Staining Solution (BioLegend, Cat# 420404) for 30 min at 4°C protected from light and sorted by fluorescence-activated cell sorting (FACS) using

(5) Chenodeoxycholic acid restrains tumor growth via TGR5-dependent type 1 dendritic cells cross-primingSignal Transduct Target TherJuly 27, 2026Fanlian Zeng, Fulei Zhao, Pei Zhou, Ya Li, Xiaoyan Wang, Yawen Hu, Jiadong Yu, Guolin Li, Chengcheng Yue, Yuting Feng, et al.cDC1 cells were incubated with 20 μg/mL OVA-FITC at 37 °C for 3 h. Dead cells were labeled with 7-AAD (420404, BioLegend), and the mean fluorescence intensity (MFI) of OVA-FITC in live cells was measured via flow cytometry

(6) EMT activates ER-to-Golgi trafficking through upregulation of REEP2 to promote lung cancer progressionResearchSquareJuly 22, 2026Guan-Yu Xiao, Oluwafunminiyi Obaleye, Oluwafunminiyi Obaleye, Shike Wang, Jiang Liu, Jonathan M. Kurie, Jonathan M Kurie, William K. Russell, Xiaochao Russell, Xiaochao Xiaoflow cytometer (BD Symphony). Cell apoptosis assay Cells were harvested and then incubated with 7-AAD (#420404, BioLegend) and Annexin-V (#640926, BioLegend) at room temperature for 10–20 min. After incubation,

(7) Protocol for high-throughput droplet-engineered TIL-retaining tumor organoids in triple-negative breast cancerSTAR ProtocJuly 17, 2026Xiaolong Zhou, Yu Zhu, Wanlong Wang, Yongde Cai, Zhiyuan Liu, Yingshi Su, Shaohua Ma9003-98-9 Cell Staining Buffer,BioLegend,Cat# 420201 7-AAD Viability Staining Solution,BioLegend,Cat# 420404 "IL-2 protein, Mouse",MCE,Cat# HY-P7077 MACS® Tissue Storage Solution,Miltenyi Biotec,Cat# 130-100-008 ,, Experimental models

(8) Unbiased avidity-based isolation of antigen-specific T cellsJ Immunother CancerJuly 8, 2026Amanda Montoya, Meredith L Frank, Peixin Jiang, Hui Nie, Minying Zhang, Emily Bontekoe, Jared K Slone, Ludovica L Posta, Sofia Rosy Caterina Sorice, Tina Cascone, et al.used, and immediately prior to flow cytometric analysis, 7AAD viability staining solution (BioLegend, #420404) was added to cells. Tetramers were provided by the Baylor College of Medicine MHC Tetramer Production

(9) CD117 epitope-shielded hematopoietic stem cell transplantation with toxin-free conditioning and in vivo selection ameliorates a β-thalassemia modelbioRxivJuly 8, 2026Romina Marone, Rosalba Lepore, Kiriaki Paschoudi, Jessica Zuin, Alessandro Sinopoli, Anna Camus, Thomas Burgold, Ewelina Bartoszek, Diego Calabrese, Mylène Toranelli, et al.antibody Goat anti-human IgG (Cat# A11013, Thermo Fisher) for 30 min at 4 °C. 7-AAD viability dye (Cat# 420404, Biolegend) was added for the final 10 min at room temperature (RT) in the dark. Flow cytometric acquisition

(10) The embryonic origins of site-specific arthritisNature ImmunologyJuly 1, 2026Sarah Davidson, Davide Simone, Kathrin Jansen, Max Cowan, Caio Machado, Ian Reekie, Ananya Bhalla, Rowie Borst, Cesar Prada Medina, Joshua Bull, et al.preparation and sequencing Where possible, cells were sorted for viability using 7-AAD dye (Biolegend, 420403) and loaded onto the 10x Genomics Chromium Controller (Chip G) following the manufacturer’s protocol

(11) Polystyrene Nanoplastics Induce Early Mitochondrial Dysfunction in H9c2 Cardiomyoblasts Without Substantial Cell DamageAntioxidants (Basel)June 26, 2026Ming-Hung Shen, Pei-Hsuan Lu, Ting-Yu Tsai, Eddy Owaga, Yi-Sheng Tsai, Chia-Wen Chen, Rong-Hong Hsiehcontaining 5% (v/v) FBS and 0.1% 7-aminoactinomycin D (7-AAD, BioLegend, San Diego, CA, USA; Cat. No. 420403). Fluorescence signals were analyzed by flow cytometry using a BD FACSDiscover S8 cell sorter (BD Biosciences,

(12) Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma developmentNature CommunicationsJune 26, 2026Masato Saito, Fumie Nakasuka, Nao Sankoda, Yihan Wang, Jumpei Taguchi, Sho Ohta, Yosuke Yamada, Manabu Ozawa, Satoko Sakurai, Atsushi Kondo, et al.the CD31⁻CD45⁻TER119⁻ fraction after dead cells were excluded using 7-AAD (1:200; BioLegend, Cat. No. 420403). Sorted cells were immediately collected in Buffer RLT Plus (Qiagen) and stored at −80 °C until RNA

(13) A Phase 2 Feasibility Study Combining Pembrolizumab and Metformin in patients with Metastatic Head and Neck CancerClin Cancer ResJune 25, 2026Olga Zamulko, Sam Nusbaum, Andrew Osterburg, McKenzie Crist, Maria Lehn, Ilaina Monroe, Audrey Romano, Casey L. Allen, Allie Forsythe, Muhammed Kashif Riaz, et al.4 hours before being collected. The cells were stained with 7AAD Viability Staining Solution (cat. #420404, Biolegend) and immediately analyzed on a 4-laser BD Fortessa Instrument. Target cells were considered

(14) Targeting non-canonical antigens unlocks functional T-cell responses in renal cell carcinomaJ Immunother CancerJune 24, 2026Jiahao Wang, Yihao Zhu, Xinyu He, Shenyi Yin, Yang He, Pengju Yao, Juan Li, Xuwen Li, Peiting Shi, Renzhi Qian, et al.interferon (IFN)-γ ELISpot assay section. Human TruStain FcX (BioLegend, Cat#422302), 7-AAD (BioLegend, Cat#420403), anti-human CD3-PE (BioLegend, Cat#300907), anti-human CD8-APC (BioLegend, Cat#300912), and anti-human

(15) Increased Arrhythmic Risk in Obesity Is Transduced by Adipose Tissue–Derived Extracellular VesiclesJACC: Basic to Translational ScienceJune 22, 2026Worawan B. Limpitikul, Marta Garcia-Contreras, Paul Spangler, Michael J. Betti, Quanhu Sheng, Steffen Pabel, Samuel Sossalla, Emeli Chatterjee, Ling Xiao, Patrick T. Ellinor, et al.anti-mouse CD206 (Biolegend, 141729, 1:50). 7-aminoactinomycin viability staining solution (Biolegend, 420403, 1:16) was used as viability marker. Then, stained cells were washed with MACS buffer (0.5% bovine serum

(16) Inducible T-bet deletion reveals tissue-specific requirements in NKp46+ ILC immunobiology and response to murine cytomegalovirusNature CommunicationsJune 18, 2026Pamela Wong, Yeeun Paik, Lily Chang, Allison Burdi, Carly Neal, Celia C. Cubitt, Jennifer Tran, Bishan Bhattarai, Lyra Morina, Nancy D. Marin, et al.RMA-S targets at the indicated E:T ratios for 4-6 h. Dead cells were identified via 7-AAD (Biolegend 420404) staining and specific killing calculated as: % 7-AAD+effector+target - % % 7-AAD+target alone. Phospho-STAT4

(17) Fungal metabolite-based immunotherapy overcomes tumor-associated macrophage immunosuppressionCell Reports MedicineJune 16, 2026Jing Hu, Xiaoman Ju, Biping Deng, Kaicheng Tang, Dongchen Yuan, Xiaofan Sun, Lingmei Kong, Yixin Xu, Zhuo Fu, Stephane Koda, et al.#P0013B CellTrace™ Violet,Invitrogen,Cat # 2286861 CSFE,Beyotime,Cat #C0051 7AAD (Clone N/A),BioLegend,Cat # 420404 Zombie Aqua™ (Clone N/A),BioLegend,Cat # 423102 123 count eBeads ebioscience,Thermo Fisher,Cat # 01-1234-42 Human

(18) Efficacy and immunogenic effects of Tumor Treating Fields ( TTFields ) in preclinical models of pancreatic ductal adenocarcinoma, with and without gemcitabine/nab‐paclitaxelInternational Journal of CancerJune 15, 2026Tal Kan, Tharwat Haj Khalil, Yiftah Barsheshet, Tali Voloshin, Lilach Koren, Bella Koltun, Cfir David, Kerem Wainer‐Katsir, Avital Vorontsova, Boris Brant, et al.(FITC)‐conjugated Annexin V (AnnV) and 7‐Aminoactinomycin D (7‐AAD) as per manufacturer's instructions (640945 and 420404, BioLegend). Cells were acquired by flow cytometry, and percentages of live (AnnV−/7AAD−), apoptotic

(19) A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains a homeostatic Th17 cell stateImmunityJune 12, 2026Dandan Yang, Linglin Huang, Hanning Cheng, Nandini Acharya, Davide Mangani, Yong Hu, Dan Hu, Alissa J. Nelson, Jian Min Ren, Vicky Yang, et al.proteins" Zombie UV fixable viability dye,BioLegend,Cat# 423108 7-AAD Viability Staining Solution,BioLegend,Cat# 420404 CountBright™ Absolute Counting Beads,Thermo Fisher Scientific,Cat# C36950 CellTrace Violet Cell Proliferation Kit

(20) Development of LRRC15-binding disulfide-constrained peptides for PET imaging of cancer-associated fibroblastsNature CommunicationsJune 5, 2026Benjamin J. Tombling, Fei Cai, Timothy J. Wendorff, Annie Ogasawara, Herman S. Gill, Jeff N. Tinianow, Andrew Chang, Aaron T. Balana, Lingling Peng, Stephen E. Miller, et al.1:500), CD45-BUV395 (BD #564279; 1:200), CD24-BUV737 (E-Bioscience #48-0242-82; 1:100), 7AAD (Biolegend #420403; 1:100) and Calcein Violet (Thermo #C34858; 1:1000). Data were acquired using a BD FACSymphony flow cytometer

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(1)

4/17/2023Easy to Use Viability Staining Solution for Live and Dead Cell Detection Soheil Tavakolpour7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.
(1)

3/24/2023Very Good 7AAD Viability Staining for Sorting Im Hong SunOur lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout ... Read Review
(1)

7/7/2022Easy to Use 7AAD Viability Stain Matthew RatsepI use this reagent to identify live/dead cells in flow cytometry experiments. The reagent is easy to use and gives good results. The only issue is that the emission spectrum is right in the middle of ... Read Review
(1)

3/13/2019Staining Human T Cells with 7-AAD Adam GuessThis reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ... Read Review
(1)

8/3/20187AAD Works Well to Determine Viability by Flow Cytometry Suzanne TomchuckIn order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD ... Read Review
(1)

10/29/2015Cell Viability Stain Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

7-AAD Viability Staining Solution from BioLegend

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7-AAD Viability Staining Solution from BioLegend

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