BioLegend
7-AAD Viability Staining Solution
420404
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Browse Similar Products: 7-AAD Cell Viability Reagents
In order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD from BioLegend works well and cost less than from other vendors. We usually test our reagents using a positive control, which are cells treated for 2-4 hours with 0.8mM hydrogen peroxide to induce cell death.
Flow Cytometry
K562 cells treated for 2 hours with 0.8mM hydrogen peroxide or untreated control
7AAD for 15 minutes at room temperature
none
We see a clear decrease in cell viability when K562 cells are treated with hydrogen peroxide (red) versus untreated cells (black).
Easy to use
Works as well as 7AAD from other vendors and is lower in price. I will continue to purchase 7AAD from BioLegend.