Staining Human T Cells with 7-AAD

March 13, 2019

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

7-AAD

Catalog Number:

420404

This reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the staining panel, and BioLegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T cells

Primary Incubation

5 µL per test in 100 µL total staining volume for 15 minutes at room temperature.

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

7-AAD staining was as expected.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well. Reproducible results. Low price.

The Bad

N/A

The Bottom Line

Good staining and reproducible results. Will continue to use this product for 7-AAD staining.

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