Easy to Use Viability Staining Solution for Live and Dead Cell Detection

Dana-Farber Cancer Institute
CIV
Postdoctoral Researcher

Overall

Quality of Results

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Company:

BioLegend

Product Name:

7-AAD Viability Staining Solution

Catalog Number:

420404

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.

Experimental Design and Results Summary

Application

Detection of Live and Dead cells in flow cytometry panel

Starting Material

N/A

Tips

Add 2-5ul per 1M cells a d wait for 10 minutes

Results Summary

It is a very easy way to exclude dead cells from the flow cytometry panel. Positive cells are dead cells, which could be gated out.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Sensitive and easy to use.

The Bad

None

The Bottom Line

In general, I recommend this more than other live/dead colors. It is very easy to use and start to work few minutes after adding to the cells.

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