Anti-Macrophage Migration Inhibitory Factor antibodies are used for the immunodetection of the protein encoded by the MIF gene. In humans, the canonical protein has a reported length of 115 amino acid residues and a mass of 12.5 kDa. Its subcellular localization is in the cytoplasm and is secreted. A member of the MIF protein family, it is known to be a pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The gene encoding this protein is implicated in Rheumatoid arthritis systemic juvenile. Other names for this target antigen include GLIF, MMIF, L-dopachrome isomerase, L-dopachrome tautomerase, epididymis secretory sperm binding protein, and GIF. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 150 citations in the literature describe the use of Macrophage Migration Inhibitory Factor antibodies in research. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.