Fig 1: Western blotting validation of expression levels of target proteins found to be differentially expressed among H, SA, and A follicles by proteomics. (A) Validation of the proteome quantification. Protein levels of beta catenin, laminA/C, inhibin alpha, HSD17B1, and MIF were detected by Western blotting in granulosa cells from healthy, slightly atretic, and atretic porcine follicles. The histogram shows the quantitative analysis (mean ± SD) of the results of three independent Western blots. The bars are labeled with completely different letters (a, b, c) indicating significant difference, P < 0.05. (B) Immunohistochemical detection of MIF expression in pig healthy, slightly atretic, and atretic porcine follicles. (C) MIF is primarily expressed in granulosa cells of primordial follicles, primary follicles, secondary follicles, and small antrum follicles. (D) MIF is also expressed in cumulus cells. (E) MIF concentrations in follicular fluid from H, SA, and A follicles. (F) MIF mRNA levels in GCs from H, SA, and A follicles. H, healthy follicle; SA, slightly atretic follicle; A, atretic follicle; BM, basement membrane; GC, granulosa cell; TC, theca cell. Scale bar is 100 µm. The data are representative of at least three independent experiments.
Supplier Page from Abcam for Anti-MIF antibody