MIF Antibody AF-289-PB from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMIF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-289-PB
ApplicationsAffinity Assay,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Neutralization,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready,ELISA Capture
HostGoat
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Porcine,Rat
TargetMIF
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human MIF, Pro2-Ala115, Accession # AAA36315
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namemacrophage migration inhibitory factor
NCBI Gene AliasesGIF, GLIF, MMIF

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse, and rat MIF in Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Targeting fibroblast activation protein in solid tumors via LNP-mediated CAR-mRNA delivery promotes durable regression in murine modelsScientific ReportsDecember 10, 2025Sikun Meng, Tomoaki Hara, Tetsuya Sato, Shotaro Tatekawa, Yasuko Arao, Yoshiko Saito, Toshiro Hirai, Daisuke Motooka, Sarah Rennie, Taroh Satoh, et al.Cat# ab289891; 5 μg per 25 g body weight) and goat anti-MIF (Bio-Techne, Minneapolis, MN, USA; Cat# AF-289-PB; 5 μg per 25 g) were used. All other procedures, including tumor model establishment, treatment regimen,

(2) Lacticaseibacillus rhamnosus R0011 secretome attenuates Salmonella enterica serovar Typhimurium secretome-induced intestinal epithelial cell monolayer damage and pro-inflammatory mediator production in intestinal epithelial cell and antigen-presenting cell co-culturesFrontiers in microbiologyJanuary 1, 2022Jeffrey MP, MacPherson CW, Tompkins TA, Green-Johnson JM.al., 2020b). For some challenges, cells were also cultured with an antibody specific for human MIF (AF-289-PB) (0.05 μg/mL) (R&D Systems) or with goat IgG isotype controls (AB-108-C) (0.05 μg/mL) (R&D Systems).

(3) Targeting the MIF/CXCR7/AKT Signaling Pathway in Castration-Resistant Prostate CancerMolecular cancer research : MCRJanuary 1, 2019Rafiei S, Gui B, Wu J, Liu XS, Kibel AS, Jia L.Cell Signaling Technology; anti-β-Actin (sc-47778) and anti-β-Tubulin (sc-80011) from Santa Cruz Technology; anti-MIF (AF-289-PB) from R&D Systems. Cell viability assay PCa cells were seeded in 96-well plates (1×104 cells/well)

(4) An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV InfectionStem cell reportsAugust 14, 2018Lang J, Cheng Y, Rolfe A, Hammack C, Vera D, Kyle K, Wang J, Meissner TB, Ren Y, Cowan C, et al.

(5) Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJanuary 30, 2018Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ.

(6) MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cellsCytokineSeptember 1, 2014DiCosmo-Ponticello CJ, Hoover D, Coffman FD, Cohen S, Cohen MC.

(7) Neuroblastoma-derived TGF-β1 modulates the chemokine receptor repertoire of human resting NK cellsJournal of immunology (Baltimore, Md. : 1950)May 15, 2013Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias MV, Moretta A, Bottino C.

(8) Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAsStem CellsSeptember 1, 2012Fonsato V, Collino F, Herrera MB, Cavallari C, Deregibus MC, Cisterna B, Bruno S, Romagnoli R, Salizzoni M, Tetta C, et al.http://www.abcam. com) and anti-E2F-2 (Santa Cruz) and goat anti-macrophage migration inhibitory factor (MIF, AF-289-PB, R&D Systems, Minneapolis, MN, http://www.rndsystems.com). Paraffin sections were subject to antigen

(9) Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositisProceedings of the National Academy of Sciences of the United States of AmericaJuly 19, 2011Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S.

(10) Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational ageThe American journal of pathologyJune 1, 2011de Oliveira Gomes A, de Oliveira Silva DA, Silva NM, de Freitas Barbosa B, Franco PS, Angeloni MB, Fermino ML, Roque-Barreira MC, Bechi N, Paulesu LR, et al.

(11) Thrombin induces macrophage migration inhibitory factor release and upregulation in urothelium: a possible contribution to bladder inflammationPloS oneDecember 31, 2010Vera PL, Wolfe TE, Braley AE, Meyer-Siegler KL.overnight with affinity purified goat anti-human MIF polyclonal antibody (R&D Systems; Minneapolis, MN; AF-289-PB; 1/1000 dilution) at C, followed by incubation with an anti-goat secondary antibody labeled with infrared

(12) Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cellsJournal of immunology (Baltimore, Md. : 1950)November 1, 2010Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM.

(13) Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivationCancer lettersApril 1, 2010Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL.fractions was performed as described previously using an affinity purified biotinylated MIF antibody (AF-289-PB, R&D Systems, Minneapolis, MN) and a near-infrared 800 continuous wave (CW) labeled strepavidin [12].

(14) Substance P Increases Cell-Surface Expression of CD74 (Receptor for Macrophage Migration Inhibitory Factor): In Vivo Biotinylation of Urothelial Cell-Surface ProteinsMediators of InflammationMarch 22, 2009Katherine L. Meyer-Siegler, Shen-Ling Xia, Pedro L. Verawith 5 μg of the corresponding antibody (CD74, Santa Cruz Biotechnology, sc-5438; or MIF, R&D Systems, AF-289-PB) at 4°C. Protein A-agarose beads (50% slurry) were added to the mixture, which was incubated for 1 hour

(15) Up-regulation of vascular endothelial growth factor-D expression in clear cell renal cell carcinoma by CD74: a critical role in cancer cell tumorigenesisJournal of immunology (Baltimore, Md. : 1950)November 1, 2008Liu YH, Lin CY, Lin WC, Tang SW, Lai MK, Lin JY.assay After treatment with or without the appropriate siRNA mentioned above, or Abs MIF (catalog no. AF-289-PB; R&D Systems), CD74 (catalog no. sc5438; Santa Cruz Biotechnology), VEGF-D (catalog no. MAB286; R&D Systems),

(16) Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factorEndocrinologyNovember 1, 2008Gilliver SC, Ruckshanthi JP, Hardman MJ, Nakayama T, Ashcroft GS.

(17) Inhibition of macrophage migration inhibitory factor or its receptor (CD74) attenuates growth and invasion of DU-145 prostate cancer cellsJournal of immunology (Baltimore, Md. : 1950)December 15, 2006Meyer-Siegler KL, Iczkowski KA, Leng L, Bucala R, Vera PL.in Western blotting: affinity purified goat anti-human MIF polyclonal Ab was obtained from R&D Systems (AF-289-PB); goat polyclonal anti-human CD74 was obtained from Santa Cruz Biotechnology (sc-5438); and monoclonal

(18) Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alphaThe Journal of biological chemistrySeptember 15, 2006Daryadel A, Grifone RF, Simon HU, Yousefi S.

(19) Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestationBiology of reproductionApril 1, 2005Paulesu L, Cateni C, Romagnoli R, Ietta F, Dantzer V.

(20) High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?Critical care medicineMarch 1, 2004Chuang CC, Hung CJ, Tsai MC, Yeh TM, Chuang YC.

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MIF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (23)

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MIF Antibody from R&D Systems, a Bio-Techne Brand

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