Fig 1: The effect of HSA-Trx on myocardial oxidative stress and inflammation in the hearts of Dox-induced cardiomyopathy model mice. (A) Representative photomicrographs of immunostaining for myocardial 8-OHdG (8-hydroxy-2'-deoxygenase) are shown at 4 weeks after the first injection of Dox. Original magnification: ×400. Scale bars represent 100 mm. Image analysis was performed to quantify the extent and intensity of 8-OhdG staining. (B) Trx and (C) MIF expression in the heart at 4 weeks after the first injection of Dox were assessed by Western blotting. (D) TNF-a, (E) I IL-6, (F) IL-10, (G) iNOS and (H) CD206 mRNA expression in the heart at 4 weeks after the first injection of Dox were determined by real-time qPCR. PBS or HSA-Trx (400 nmol/kg) was administered i.v. three times a week from 2 weeks after the first injection of Dox. Results are the means ± S.E. (n = 4). * p < 0.05, ** p < 0.01 compared with the saline injection group (normal mice).