Picogram-Level Protein Detection by Western Blot

Stanford University
Department of Pathology
Postdoctoral Researcher

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Company:

ThermoScientific

Product Name:

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog Number:

34580

I study gene signaling pathways and routinely use Western blotting to measure protein expression. SuperSignal West Pico PLUS provides a sensitive and reliable chemiluminescent readout for less abundant proteins.

Experimental Design and Results Summary

Application

Western blot detection

Starting Material

Protein lysates transfered onto membranes

Tips

This substrate can be mixed with regular ECL to control the level of signal and exposure time.

Results Summary

It produced clear and stable chemiluminescent signals for samples with low protein expression without increasing the background.

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Summary

The Good

Easy to use, reliable signal, and convenient for routine Western blotting.

The Bad

Expensive

The Bottom Line

A reliable, high-sensitivity ECL substrate for Western blots with a simple workflow and consistent results.

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