Reliable Glutathione Agarose

Stanford University
Department of Pathology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

GoldBio

Product Name:

Glutathione Agarose Resin

Catalog Number:

G-250-50

I regularly express and purify GST-tagged proteins for biochemical studies. This glutathione agarose resin provides a straightforward affinity purification method for GST-tagged proteins.

Experimental Design and Results Summary

Application

Purification of GST-tagged proteins.

Starting Material

Bacterial lysates expressing GST-fusion proteins

Tips

Wash the beads before adding lysate to completely remove ethanol

Results Summary

I used the resin to efficiently capture GST-tagged proteins and obtain purified protein for downstream experiments. I could purify and recover 2-4 mg of my protein per mL of resin.

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Summary

The Good

Good binding capacity, ease of use

The Bad

Expensive

The Bottom Line

A reliable and straightforward resin for routine GST-fusion protein purification.

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