
Protein purification is a staple of most pharmaceutical, biotech, and basic research labs. Multifarious protein purification methods and systems, from immunoprecipitation to chromatography are available, all of which require meticulous sample handling and solid research tools. Fundamental considerations for devising a protein purification plan include finding the right method, reagents, and instruments. Researchers require distinct tools which depend on their end goal, such as purifying proteins for antibody production or for therapeutics. Homogenizers, buffers, and protease inhibitors are needed to extract protein from its source. Products for extracting organelle proteins are also distributed to avoid cellular total protein contamination. To increase working quantity extracted proteins can be concentrated with products such as dialysis membranes. HPLC or GC chromatography are fast, high throughput methods of protein purification. Low-scale systems, like affinity kits are available for smaller sample volume. Researchers can acquire assistance by investigating listed protein purification service and user guide providers.
Researchers are finding new ways to target proteins that once seemed out of reach
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Learn more about tools to build PROTACs and assess their efficacy
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Set up a live demo with a Bio-Rad Imaging Specialist - Request a DemoUse the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This ...
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The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
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The Abcam PEG Virus Precipitation Kit is used to concentrate lentivirus from HEK293T supernatant, and it has been a great solution for labs that don't want to tie up an ultracentrifuge.
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I work with in-vitro protein phase separation assays which require high-quality purified proteins. The cOmplete™ His-Tag Purification Resin is a vital tool in protein purification for several reasons. Its nickel-chelating technology enables specific ...
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