Invitrogen
Transferrin Receptor Monoclonal Antibody (H68.4)
13-6800
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We conducted transferrin receptor (TfR) staining in primary human brain endothelial cells (HBMECs).
Immunofluorescence
Primary human brain microvascular endothelial cells
Dilution 1:100
Donkey serum
Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647, #A-31571 (dilution 1:500)
none
Confocal
Cells were fixed in 4% PFA, and permeabilized with 0,3% Triton x-100. The optimization takes some time and it's possible to observe high background signal, compromising the visualization of the signal. Cells were countedstained with Hoescht and Ve-Cadherin for detection of nuclei and tight junctions, respectively.
Some signal can be detected
High background signal
This antibody performs very well in WB; however, IF applications may require some time and optimization to achieve optimal results.