Invitrogen™
4',6-Diamidino-2-Phenylindole, Dilactate (DAPI)
D3571
Info: Supplier Page
I used DAPI to stain nucleus of the cells for visualization in confocal microscopy and observed the localization of my protein of interest.
Confocal microscopy
Fixed Neuro2a cells
Cells require permeabilization for better staining.
Confocal microscopy images showing expression pattern of over-expressed EGFP and nucleus stained with DAPI.
N/A
Good for multiplexing with other dyes, rapid staining,and highly stable.
Cannot be used for live cell imaging.
Good nuclear stain with minimal cross-reactivity.