Novus Biologicals' CD79a Antibody Works for IHC

Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

CD79A Antibody (HM57) - BSA Free

Catalog Number:

NB100-64347

Clone Number:

HM57

Info:Supplier Page View Product Specs

Browse Similar Products: CD79a Antibodies

It is classical CD79a antibody. It stains B cell lymphocyte and some plasma cells. An antibody that also has affinity (probably nonspecific) for smooth muscle cells.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Dog lymphoma

Primary Incubation

1/100

Blocking Agent

BSA, 1% - 10'

Secondary Incubation

Anti-rabbit IgG, 1/200 dilution, RT.

Tertiary Incubation

Peroxidase-strepteavidin complex (1 hours at RT) and tehn AEC chromogen, RT 20'

Detection

ABC-peroxidase technique

Results Summary

It is essential antibody for cat and dog lymphoma Since CD20 (great b cell marker) can stain some T cell lymphoma. As seen in the photograph, it binds to muscular layer of vessels as a nonspecific reaction. But i used different brand of same antibody clone before. This muscular positivity is seen all.

DOI or PMID #

N/A

Additional Notes

Gill's hemalum as a background staining.

Image Gallery

Summary

The Good

Easy to use, specific for multiple species.

The Bad

Non-specific staining

The Bottom Line

It is an essential antibody for differential diagnosis of cat and dog tumors.

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