Reliable Detection Substrate for Highly Expressed Proteins

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Super signal west PICO plus chemiluminescent substrate

Catalog Number:

34580

We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic Aire expressing cells.

Experimental Design and Results Summary

Application

Western blotting

Starting Material

N/A

Tips

While a good general purpose substrate, this kit is not high enough sensitivity for very rare proteins.

Results Summary

This chemiluminescent substrate kit gives reliable detection of most proteins by western blot using both film and imaging systems following the manufacturer's protocol with no further optimization. However it is not a high-sensitivity substrate suitable for extremely lowly expressed proteins.

DOI or PMID #

N/A

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Summary

The Good

A good compromise between price and sensitivity for most proteins.

The Bad

Not sensitive enough for very lowly expressed proteins in your sample.

The Bottom Line

Decent substrate kit for most western blotting applications.

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