Podocalyxin Antibody AF1556 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPodocalyxin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1556
ApplicationsIHC-F,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat,Transgenic Mouse,Xenograft
TargetPodocalyxin
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
IsotypeIgG
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Podocalyxin, Ser21-Arg402, Accession # Q9R0M4
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namepodocalyxin-like, podocalyxin like
NCBI Gene AliasesGp200, gp135, PDX, PODXL1, Podxl1, PCLP, PC, Ly102, PCLP-1, Pclp1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for detecting podocalyxin in endothelial cells?

Yes, the Podocalyxin Antibody has been shown to be effective for detecting podocalyxin in endothelial cells through immunofluorescent staining, as demonstrated in studies such as Eakin et al. (2020) and Koch et al. (2021).

What species does this antibody react with?

The Podocalyxin Antibody is reactive with mouse, human, and rat species, making it suitable for a variety of experimental models.

Is there any evidence of this antibody being used in studies related to kidney diseases?

Yes, the Podocalyxin Antibody has been utilized in research related to kidney diseases, including studies on diabetic kidney disease where podocyte injury is a critical factor, as indicated in Liu et al. (2024).

How specific is this antibody for podocalyxin compared to other proteins?

The Podocalyxin Antibody exhibits less than 1% cross-reactivity with recombinant human podocalyxin and recombinant endoglycan, indicating a high specificity for its target in applications such as Western blotting and ELISA.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The Role of Dopaminergic Agonists in Geographic Atrophy: Converging Evidence From Clinical and Experimental DataTransl Vis Sci TechnolAugust 14, 2026Induvahi Veernala, Kyle S. Chan, Harrshavasan T. S. Congivaram, Andrea S. Cuamatzi-Castelan, Amrita Rajesh, Joyce Gong, Olivia C. Ide, Jeremy A. Lavine, on behalf of the SOURCE ConsortiumTarget,Fluorophore,"Manufacturer, Product #, Dilution",Use Goat anti-podocalyxin,—,"R&D Systems, AF1556,1:250",Primary Rat anti-podocalyxin,—,"R&D Systems, MAB1556,1:200",Primary Actin-Phalloidin,Alexa

(2) Modulation of miR-23b Wnt/β-catenin Axis Strengthens Endothelial Barrier PropertiesAdv Sci (Weinh)July 30, 2026Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, et al.added to tissue overnight at 4 C: anti‐NeuN (ab177487, Abcam, Waltham, MA, USA), anti‐Podocalyxin (AF1556, R&D Systems, Minneapolis, MN, USA), anti‐Glutamine synthetase (ab73593, Abcam, Waltham, MA, USA), anti‐LEF1

(3) Loss of pericytic integrin-β1 exacerbates blood-brain barrier damage and hemorrhagic brain injuryStrokeJuly 8, 2026Irem Culha-Taskin, Ava Nasrollahi, Yao Yaosections were incubated with anti-PDGFRβ (Cell Signaling, 3169S, 1:400) and anti-podocalyxin (R&D Systems, AF1556, 1:400) antibodies overnight, followed by appropriate secondary antibodies the next day. After extensive

(4) Tissue-resident macrophages maintain choroidal homeostasis by complement dependent and independent mechanismsJournal of NeuroinflammationMay 18, 2026Joyce Gong, Sanaz Ghotbaldini, Ritvik Viniak, Amrita Rajesh, Jeremy A. Lavinepodocalyxin,ࣧ,"R&D Systems, MAB1556, 1:200",Primary Ab Goat anti-mouse podocalyxin,ࣧ,"R&D Systems, AF1556, 1:250",Primary Ab Goat anti-mouse CD31,ࣧ,"R&D Systems, AF3628, 1:250",Primary Ab Rat anti-mouse CD31,ࣧ,"Novus

(5) High-fat diet feeding induces organ-specific vascular remodeling with distinct temporal dynamics in male miceCommunications BiologyMay 9, 2026Emmi Pakarinen, Satu Paavonsalo, Madeleine H. Lackman, Yelin Subashi, Hanna M. Ruddock, Sinem Karamanto three days at +4°C on a shaker. Primary antibodies used were goat anti-PDXL (1:400, R&D Systems, AF1556, RRID: AB_354858), rabbit anti-ERG (1:1000, Abcam, ab92513, RRID: AB_2630401), rabbit anti-LYVE1 (1:1000,

(6) Loss of mitochondrial DNA helicase in retinal macroglia drives neovascular retinopathyEMBO Molecular MedicineMay 8, 2026Sofiia Olander, Sinem Karaman, Fumi Suomi, Kevin Aguilar, Aleksandra Zhaivoron, Maiken Nedergaard, Lina Smeds, Jussi Tiihonen, Albert Quintana, Juan Hidalgo, et al.,Synaptic Systems,cat#234003 Rabbit anti-IBA1,Wako Chemicals,cat#019 19741 Primary goat anti-podocalyxin,R&D Systems,cat#AF1556 Rabbit anti-Sox9,Millipore,cat#AB5535 Streptavidin-conjugated Alexa Fluor 488 antibodies,Invitrogen,cat#S-32354 Donkey

(7) Innovative 3D-Image Analysis of Cerebellar Vascularization Highlights Angiogenic Gene Dysregulations in a Murine Model of Apnea of PrematurityCerebellumApril 28, 2026A. Rodriguez-Duboc, C. Racine, M. Basille-Dugay, D. Vaudry, B. Gonzalez, D. BurelAntibodies,Target,Dilution,Species,Supplier,Secondary antibodies,Dilution Podocalyxin,Capillaries,1:200,Goat,R&D Systems (#AF1556),DAG-Alexa 594,1:400 α-SMA-Cy3,Arteries,1:500,Mouse,Sigma-Aldrich (#C6198),N/A,N/A PECAM1,Arterioles,1:200,Rat,Millipore

(8) Hypoxia or tobacco-smoke exposure induce region-specific microvascular remodeling in the brainSci RepApril 17, 2026Nazli Salik Demirtas, Edma Loku, Yasmine Porschen, Julia Schäffer, Cheng-Yu Wu, Christoph Rummel, Natascha Sommer, Stefan Hadzic, Norbert Weissmann, Till Acker, et al.Biotin,Swine,Secondary,Polyclonal,Dako,E0353,, IF,IF,IF,IF,IF,IF,IF,IF anti-Iba1,Rabbit,Primary,Polyclonal,Wako Chemicals,019-19741,TE buffer pH 9,1:1000 anti-Podocalyxin,Goat,Primary,Polyclonal,R&D Systems,AF1556,Citrate buffer pH 6,1:100 anti-Fibrinogen,Rabbit,Primary,Polyclonal,Abcam,ab34269,Citrate buffer pH

(9) Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult MiceFASEB JApril 15, 2026Arttu Junnila, Kalle T. Rytkönen, Guillermo Martinez-Nieto, Mats Perk, Otto Mäkelä, Hao Li, Jenni Airaksinen, Ida Hyötyläinen, Oliver Mehtovuori, Asta Laiho, et al.,Cell Signaling Technology,2230,AB_823558,1:400,IF anti‐PAX2,Rabbit,Invitrogen,71–6000,AB_2533990,1:400,IF anti‐Calbindin,Goat,Santa Cruz Biotechnology,sc‐7691,AB_634520,1:500,IF anti‐Podocalyxin,Goat,R&D Systems,AF1556,AB_354858,1:400,IF anti‐HNF4A,Mouse,Invitrogen,MA1‐199,AB_2633309,1:50,IF anti‐Phosphohistone H3,Mouse,Cell Signaling Technology

(10) Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult MiceFASEB JApril 4, 2026Arttu Junnila, Kalle T. Rytkönen, Guillermo Martinez‐Nieto, Mats Perk, Otto Mäkelä, Hao Li, Jenni Airaksinen, Ida Hyötyläinen, Oliver Mehtovuori, Asta Laiho, et al.,Cell Signaling Technology,2230,AB_823558,1:400,IF anti‐PAX2,Rabbit,Invitrogen,71–6000,AB_2533990,1:400,IF anti‐Calbindin,Goat,Santa Cruz Biotechnology,sc‐7691,AB_634520,1:500,IF anti‐Podocalyxin,Goat,R&D Systems,AF1556,AB_354858,1:400,IF anti‐HNF4A,Mouse,Invitrogen,MA1‐199,AB_2633309,1:50,IF anti‐Phosphohistone H3,Mouse,Cell Signaling Technology

(11) Krüppel-like factor 2 regulates renin expression in mature juxtaglomerular cellsAmerican Journal of Physiology-Renal PhysiologyApril 1, 2026Hiroki Yamaguchi, Jason P. Smith, Omar Guessoum, Drishti Daga, Manako Yamaguchi, Lucas Ferreira de Almeida, Daisuke Matsuoka, Hirofumi Watanabe, Silvia Medrano, R. Ariel Gomez, et al.sections were then incubated overnight at 4°C with the Goat polyclonal anti-mouse podocalyxin antibody (AF1556 [R&D Systems, Minneapolis, MN], diluted at 1:50, RRID:AB_354858) in blocking solution. After the washing

(12) Sex-specific APOE4-dependent innate immunity regulates meningeal lymphatics, brain lipids, neuroinflammation, and cognitionNeuronMarch 26, 2026Nickoleta Delivanoglou, Kennedi T. Todd, Francisco Almeida, Shanon Rego, Amogh Changavi, Myriam Spajer, Virginia Estades Ayuso, Liliana M. Pinho-Correia, Guadalupe Sanchez, Sofia P. das Neves, et al.LYVE-1,AngioBio,Cat#11-034 Goat anti-mouse CD31,R&D Systems,Cat#AF3628 Goat anti-mouse Podocalyxin,R&D Systems,Cat#AF1556 "Rat anti-mouse CD206, clone MR5D3",Bio-Rad Laboratories,Cat#MCA2235 Rabbit anti-mouse hAPOE,Cell Signaling Technology

(13) The 129S1/SvlmJ mouse strain recapitulates severe hypertensive target organ damage under moderate angiotensin II–induced hypertensionScientific ReportsMarch 5, 2026Arthur Orieux, Romain Boulestreau, Marie-Lise Bats, Maxime Michot, Alexandre Boyer, Virginie Dinet, Juliette Vaurs, Pascale Dufourcq, Claire Peghaire, Cécile Duplaa, et al.antibodies: GFAP (1:400, ThermoFisher Scientific, 130300, MA, USA), PODOCALYXIN (1:400, R&D Systems, AF1556, Minneapolis, MN, USA), IBA1 (1:400, Fujifilm Wako, 019–19741, Osaka, Japan), CD206 (1:400, R&D Systems,

(14) Effects of Repeated Early-life Anesthesia Exposure on Visual System Development in MiceAnesthesiologyMarch 1, 2026Célia Seillier, Amandine Élodie Bonnet, Shahad Albadri, Nicolas Poirel, Gilles Orliaguet, Denis Vivien, Jean-Philippe SalaünNo.,Species,Dilution,Clone,Conjugate Antibodies used in immunohistochemistry,,,,,, Podocalyxin,Biotechne,AF1556,Goat,1:200,, Vascular cell adhesion molecule 1 (VCAM-1),BD Biosciences,553330,Rat,1:200,, Ionized

(15) A Highly Active Angiopoietin 1 Mimetic Potentiates Angiogenesis in Mouse Models of Choroidal NeovascularizationTransl Vis Sci TechnolFebruary 12, 2026Asmaa A. Youssif, Pan Liu, Danille Gaczkowski, Susan E. Quaggin, Jing Jin, Benjamin R. ThomsonImaging Whole-mount choroid immunostaining was performed as described above, using antibodies against Podocalyxin (AF1556; R&D Systems, Minneapolis, MN, USA), Pecam1 (AF3628; R&D systems), HA-tag (C29F4; Cell Signaling, Danvers,

(16) Oriented cell divisions induce basal progenitors and regulate neural expansion across tissues and speciesScience advancesFebruary 6, 2026Boulan B, Lacomme M, Benadjal A, Krueger M, Currie K, La Torre A, Chédotal A, Cayouette M.,Millipore Sigma,AB2237 PAR3,Rabbit,1/200,EMD Millipore,07-330 Parvalbumin,Rabbit,1/1000,Swant,PV27a PODXL,Goat,1/500,R&D Systems,AF1556 pH3,Rabbit,1/400,EMD Millipore,06-570 PTPRZ1,Rabbit,1/500,Millipore Sigma,HPA015103 SOX2,Rabbit,1/200,Abcam

(17) Astrocyte CB1 receptors drive blood-brain barrier disruption in central nervous system inflammatory diseaseJournal of NeuroinflammationJanuary 29, 2026Teresa Colomer, Ana Bernal-Chico, Ester Sánchez-Martín, Alvaro Moreno-García, Andrés Mateo Baraibar, Aitziber Uribe-Irusta, Ander Iriarte-Sarria, Sandra Beriain, Urszula Skupio, Charlotte Gatuingt-Chasseriaud, et al.Adhesion Molecule 1 (ICAM-1; 1:500; R&D Systems; #AF796), Podocalyxin (PODXL; 1:500; R&D Systems; #AF1556), Vascular cell adhesion protein 1 (VCAM-1; 1:1000; Abcam; #ab134047), zona occludens (ZO-1; 1:1000;

(18) Low and High Pressor Doses of Ang II Lead to Two Distinct Phenotypes of Hypertensive Heart Disease in MiceAPMISJanuary 6, 2026Diana Törmä, Tuisku Suoranta, Mimmi Rinta‐Harri, Jarkko Hytönen, Seppo Ylä‐Herttuala, Anna‐Kaisa Ruotsalainentissues were stained with HE‐stain. Additionally, cardiac tissues were stained with Podocalyxin (1:500; AF1556, goat anti‐mouse, R&D systems, USA), α‐smooth muscle actin (α‐SMA, 1:200, #19245, rabbit anti‐mouse,

(19) Combined HIF-1α blockade and CHIR99021 treatment reverses pulmonary fibrosis via modulation endothelial-to-mesenchymal transitioniScienceDecember 19, 2025Jae-Kyung Nam, Seo-Hyun Choi, Ji-Hee Kim, Kyu Jin Choi, Hae-June Lee, Jeeyong Lee, Haeng Ran Seo, Yoon-Jin Leeβ-Catenin (1:100; Cell signaling; #8480), CA9 (1:200; Novus; # NB100-417) and Podocalyxin (1:200; R&D; AF1556). For quantification, a minimum of five images per tissue section were acquired, and positively stained

(20) Protocol for generation of endothelial cells from human-derived iPSCs and integration into mouse brain explantsSTAR ProtocDecember 5, 2025Iza Erzar, Nora Noll, Roberta Lugano, Anna Dimberg, Maximiliano Arce, Peetra U. Magnussondilution),Sigma-Aldrich,C6198; RRID: AB_476856 Goat anti-Podocalyxin (1:100 dilution),R&D Systems,AF1556; RRID: AB_354858 Rabbit anti-VE-cadherin (1:100 dilution),Cell Signaling Technology,2500; RRID: AB_10839118 Goat

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3/27/2023Great Mouse Podocalyxin Antibody for Immunofluorescence Troy TrevinoBlood vessels in mouse cerebellum were identified by immunofluorescence by staining for podocalyxin. Leakage of fibrinogen into the brain parenchyma identified areas of blood-brain barrier damage.
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3/6/2023Podocalyxin as Vessel-Marker in Mouse Brain Ali AlmousawiPodocalyxin was detected in formalin fixed paraffin embedded sections of mouse brain using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at a dilution of... Read Review
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5/6/2021Workable Antibody to Label Endothelial Cells Abhijit NirwaneOur lab works on basement membrane and its role in blood-brain barrier maintenance. We used this antibody to stain mouse brain endothelial cells in vivo.