TRYPLE EXPRESS 100ML from Thermo Fisher Scientific

Product Specs
ItemTRYPLE EXPRESS 100ML
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number12604013
Quantity100mL
Concentration1 X
FormatAnimal Origin-Free
RangeCheck and Record
ApplicationsCELL CULTURE
Brand/TrademarkGibco™
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

Specifications/Features

Trypsin Replacement

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolismJ Extracell VesiclesDecember 27, 2023Hui Gao, Yuxiao Zeng, Xiaona Huang, Luodan A, Qingle Liang, Jing Xie, Xi Lin, Jing Gong, Xiaotang Fan, Ting Zou, et al.hESCs reached 80% confluence, they were dissociated into single‐cell suspensions using TrypLE Express (12604013; Gibco) containing 0.05 mg/mL DNase I (03724778103; Roche, Switzerland) and 20 mM Y‐27632 (SCM075; Merck,

(2) A Fluorescence-Based Sensor for Calibrated Measurement of Protein Kinase Stability in Live CellsbioRxivDecember 8, 2023Joseph W. Paul, Serena Muratcioğlu, John KuriyanFlow cytometry and fluorescence activated cell sorting Cells were released from plates with TrypLE (Gibco #12604013) at 37°C for five to ten minutes. Cells were then diluted in three volumes of flow cytometry buffer

(3) Sperm chromatin accessibility's involvement in the intergenerational effects of stress hormone receptor activationTransl PsychiatryDecember 8, 2023Vincent Fischer, Miriam Kretschmer, Pierre-Luc Germain, Jasmine Kaur, Sergio Mompart-Barrenechea, Pawel Pelczar, David Schürmann, Primo Schär, Katharina Gapplipofectamine2000 overnight. The next day, cells were washed with 1x PBS and harvested with 1 mL TryplE (Gibco, 12604013), then snap-frozen in liquid nitrogen. RNA extraction and q-RT-PCR RNA was extracted from liver and

(4) Visualizing DNA replication by single-molecule analysis of replicated DNASTAR ProtocDecember 3, 2023Advaitha Madireddy, Jeannine GerhardtScientific,Cat #15568-025 Tris-HCl pH 7.5 (TE 7.5),Invitrogen,Cat #X100 Tris-HCl pH 8.0 (TE 8),Invitrogen,Cat #12604013 Triton X-100,Sigma-Aldrich,Cat #T8154 Trypan blue,Sigma-Aldrich,Cat #T8154 YOYO-1 iodide (491/509),Thermo Fisher Scientific

(5) Protocol for isolation of signet ring cells from human gastric mucosaSTAR ProtocolsDecember 1, 2023Sarah G. Samaranayake, Lauren A. Gamble, Cassidy Bowden, Benjamin L. Green, Amber F. Gallanis, Dilara Akbulut, Niharika Shah, Jonathan M. Hernandez, Jeremy L. Davis,Miltenyi Biotec,Cat#130-100-008 "Gibco TrypLE express enzyme (1×), no phenol red",Thermo Fisher Scientific,Cat#12604013 "HistoGel specimen processing gel, Epredia",VWR,Cat#83009-992 ,, Critical commercial assays,Critical

(6) Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysisSTAR ProtocolsDecember 1, 2023Aikaterini Skorda, Anna Røssberg Lauridsen, Kaisa Huhtinen, Alexandra Lahtinen, Wojciech Senkowski, Jaana Oikkonen, Johanna Hynninen, Sampsa Hautaniemi, Tuula Kallunki(HPLM),Gibco,Cat# A4899101 Phosphate-buffered saline (PBS),Gibco,Cat# 10010023 TrypLE Express,Gibco,Cat# 12604013 "HEPES, pH 7.4",Homemade,N/A 2-Hydroxybutyric acid,Sigma-Aldrich,Cat# 220116-5G Primocin,InvivoGen,Cat#

(7) Optogenetic induction of alpha-synuclein aggregation in human dopaminergic neurons to model Parkinson’s disease pathologySTAR ProtocolsDecember 1, 2023Eun A. Ra, Min Seong Kim, Gabsang Lee11330057 KnockOut Serum Replacement,Gibco,Cat# 10828028 KnockOut™ DMEM,Gibco,Cat# 10829018 TrypLE™,Gibco,Cat# 12604013 mTeSR™1,STEMCELL Technologies,Cat# 85857 Neurobasal™ Medium,Gibco,Cat# 21103049 PBS,Corning,Cat#

(8) Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironmentNature CommunicationsNovember 30, 2023Ning Li, Qin Zhu, Yuhua Tian, Kyung Jin Ahn, Xin Wang, Zvi Cramer, Justine Jou, Ian W. Folkert, Pengfei Yu, Stephanie Adams-Tzivelekidis, et al.S7067-5MG). The organoids were passaged biweekly by digesting with TyrpLE (Thermo Fisher Scientific, 12604013) for 5 min at 37 °C and then splitting in 1:3-5 ratio. The medium was supplemented with 10 uM Y-27632

(9) The Influence of Nucleoside Reverse Transcriptase Inhibitors on Mitochondrial Activity, Lipid Content, and Fatty-Acid-Binding Protein Levels in Microglial HMC3 CellsPharmaceuticals (Basel)November 29, 2023Katarzyna Lipke, Adriana Kubis-Kubiak, Agnieszka PiwowarWhen the cells reached 80–90% confluence, they were subcultured using TrypLETM Express Enzymesolution (12604013) from Gibco, Thermo Fisher Scientific, Waltham, MA, USA. 4.2. Tested Compounds The concentrations

(10) Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2Science AdvancesNovember 24, 2023Gabriele Colozza, Heetak Lee, Alessandra Merenda, Szu-Hsien Sam Wu, Andrea Català-Bordes, Tomasz W. Radaszkiewicz, Ingrid Jordens, Ji-Hyun Lee, Aileen-Diane Bamford, Fiona Farnhammer, et al.was removed. The organoid/cell pellets were resuspended in 500 μl of TrypLE Express Enzyme (Gibco, 12604013) and incubated at 37°C for 5 min. After incubation, the mixtures were pipetted up and down a few times

(11) Embryo-scale reverse genetics at single-cell resolutionNatureNovember 23, 2023Lauren M. Saunders, Sanjay R. Srivatsan, Madeleine Duran, Michael W. Dorrity, Brent Ewing, Tor H. Linbo, Jay Shendure, David W. Raible, Cecilia B. Moens, David Kimelman, et al.with forceps and transferred to a 10 cm petri dish containing 1× TrypLE (Thermo Fisher, catalogue no. 12604013). Using a widebore tip, embryos were transferred, one by one, into separate wells of a 96-well V-bottom

(12) Accessible high-throughput single-cell whole-genome sequencing with paired chromatin accessibilityCell Reports MethodsNovember 20, 2023Konstantin Queitsch, Travis W. Moore, Brendan L. O’Connell, Ruth V. Nichols, John L. Muschler, Dove Keith, Charles Lopez, Rosalie C. Sears, Gordon B. Mills, Galip Gürkan Yardımcı, et al.inactivated",VWR,SH30073.04HI "Penicillin/Streptomycin (10,000 U/ml)",Life Technologies,15-140-122 TrypLE Express Enzyme,ThermoFisher Scientific,12604013

(13) Simultaneous Profiling of Chromosome Conformation and Gene Expression in Single CellsBIO-PROTOCOLNovember 20, 2023Yujie Chen, Heming Xu, Zhiyuan Liu, Dong Xing10010049)Fetal bovine serum (FBS) (Gibco, catalog number: 10099141C)TrypLETM Express (Gibco, catalog number: 12604013)Sucrose, reagent grade, 99% (Sigma, catalog number: V900116-500G)HEPES, BioUltra (Sigma, catalog number:

(14) Characterization of Rickets Type II Model Rats to Reveal Functions of Vitamin D and Vitamin D ReceptorBiomoleculesNovember 19, 2023Yuichiro Iwai, Ayano Iijima, Satoko Kise, Chika Nagao, Yuto Senda, Kana Yabu, Hiroki Mano, Miyu Nishikawa, Shinichi Ikushiro, Kaori Yasuda, et al.the resulting skin samples were incubated in 5 mL of Tryp Express (Thermo Fisher, Waltham, MA, USA, #12604013) for 15 min at 37 °C, and then 25 mL of CnT-07 medium was added, and the samples were vigorously pipetted

(15) WIP1 is a novel specific target for growth hormone actioniScienceNovember 17, 2023Tugce Apaydin, Svetlana Zonis, Cuiqi Zhou, Christian Wong Valencia, Robert Barrett, Ger J. Strous, Jan A. Mol, Vera Chesnokova, Shlomo Melmed17504044 A83301,Tocris,Cat# 2939 SB 202190,Tocris,Cat# 1264 TrypLE,Thermo Fisher Scientific,Cat# 12604013 Cell Recovery Solution,Corning,Cat# 354253 IntestiCult Organoid Growth Medium,STEMCELL Technologies,Cat#

(16) Inhibition of EIF2α Dephosphorylation Decreases Cell Viability and Synergizes with Standard-of-Care Chemotherapeutics in Head and Neck Squamous Cell CarcinomaCancers (Basel)November 9, 2023Anna M. Cyran, Florian Kleinegger, Norbert Nass, Michael Naumann, Johannes Haybaeck, Christoph ArensCO2. Once 70–80% confluence was achieved, cells were harvested enzymatically (TrypLE Express Enzyme, #12604013, ThermoFisher Scientific, USA), centrifuged, and suspended in the supplemented medium. For chemosensitivity

(17) Dual function of angiogenin-4 inducing intestinal stem cells and apoptosisFrontiers in Cell and Developmental BiologyNovember 2, 2023Hirohito Abo, Mst. Farzana Sultana, Hiroto KawashimaFBS in PBS and washed by centrifugation at 4°C, 300 × g for 3 min. Next, 500 µL of TrypLE Express (12604013, Gibco, Waltham, MA, United States) was added to organoids and incubated at 37°C for 10 min. After incubation,

(18) Immune microenvironment dynamics of HER2 overexpressing breast cancer under dual anti-HER2 blockadeFrontiers in ImmunologyOctober 31, 2023Sofia Batalha, Catarina Monteiro Gomes, Catarina Britofor alginate dissolution, washed in PBS and afterwards incubated in TrypLE™ Express Enzyme (Gibco, #12604013) for dissociation of cancer cell aggregates. Samples were treated with Human TruStain FcX (Biolegend,

(19) Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vectorScientific ReportsOctober 28, 2023Satoko Kise, Ayano Iijima, Chika Nagao, Tadashi Okada, Miyu Nishikawa, Shinichi Ikushiro, Tomoko Nakanishi, Shigeto Sato, Kaori Yasuda, Toshiyuki Sakakiwashed with PBS to remove extra collagenase, and incubated with 5 ml of Tryp Express (Thermo Fisher #12604013) at 37 °C for 15 min, and then 25 mL of a CnT-07 medium was added and pipetted tremendously to disassociate

(20) Computer controlled expansion of equine cord blood mesenchymal stromal cells on microcarriers in 3 L vertical-wheel® bioreactorsFrontiers in Bioengineering and BiotechnologyOctober 19, 2023E. L. Roberts, B. D. Abraham, T. Dang, E. Gysel, S. Mehrpouyan, A. H. Alizadeh, T. G. Koch, M. S. Kalloswashes. Following the second wash, DPBS was removed, and 1.4 L of TrypLE Express (Thermo Fisher, Ca#12604013) was added. The bioreactor was then agitated at 19 RPM at 37°C for 15 min. After 15 min the agitation

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(1)

12/28/2023Fast and Versatile Reagent From ThermoFisher for Embryoid Body Dissociation Karl WagnerEmbryoid bodies (EBs) were formed from human embryonic stem cells for mesoderm differentiation. TryplE was used to dissociate EBs after mesoderm differentiation for monolayer culture and further ... Read Review
(1)

3/3/2023Thermo-Stable Alternative to Trypsin Vincent GiacaloneTrypsin is a common reagent for detaching adherent cells, but must be stored frozen and activity can decline over time. The TrypLE Express solution acts in a similar way to left adherent cells off of ... Read Review
(1)

8/5/2022TrypLE Express Dissociates Adherent Cell Lines for Passaging Niroj Shrestha1-2 ml TrypLE Express is added to adherent cell lines like RBL-2H3 and HL-1 for a few minutes till the cells appear round. 2 ml of cell culture medium is added and pipetted gently to dissociate the ... Read Review
(1)

11/22/2021Gentle Enzyme Solution for Trypsinization of Adherent Cells Mona AgrawalI have used this TrypLE enzyme solution for the trypsinization of stem cells. It is an animal origin free and gentle to cells which makes it preferable for stem cells research.
(1)

10/11/2019Dissociation Reagent for hPSCs Elena SeranovaStable at RT, reproducible results.
(1)

6/5/2019TrypLE Express Enzyme for Gently Detaching Cells - Gibco Ab GUse TrypLE Express for generation of cancer stem cells and easy detachment. This reagent is particularly useful for flow cytometry as detachment by other methods can reduce cell surface protein ... Read Review
(1)

4/24/2015Tryple- Alternative to trypsin Monica ValenciaThis product can be used for very fast dissociation of hard to trypsinize cells without the need to inactivate with FBS or SBTI. The tryple reagent is also very gentle on cells, it is stable at room ... Read Review

TRYPLE EXPRESS 100ML from Thermo Fisher Scientific

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Specifications/Features

Thermo Fisher Scientific

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TRYPLE EXPRESS 100ML from Thermo Fisher Scientific

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