Great Kit for Cell Viability and More

Penn State College of Medicine
Neurosurgery
Research Intern

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Cell Counting Kit 8 (WST-8 / CCK8)

Catalog Number:

ab228554

This kit is marketed as a way to measure cell viability. This is true, but it can do so much more. The assay works by measuring active mitochondria (more mitochondria, the more the dye is reduced). So, if you have reason to believe your experimental condition can impact mitochondrial function, this will be impacted by that.

Experimental Design and Results Summary

Application

Cell Viability

Starting Material

Live Cells

Tips

Be sure to properly optimize.

Results Summary

I used this kit to determine if my treatment increased the overall metabolic activity of my cells. I have also used it to measure general cell viability.

DOI or PMID #

N/A

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Summary

The Good

Works very well.

The Bad

The reliance on mitochondrial activity can be an issue.

The Bottom Line

I recommend but understand the limitations.

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