Cell Counting Kit 8 (WST-8 / CCK8) ab228554 from Abcam

Product Specs
ItemCell Counting Kit 8 (WST-8 / CCK8)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228554
Quantity1000 Test, 5000 Tests
Sample TypeAdherent cells, Suspension cells

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) hiPSC-Derived M1 Macrophages Exhibit Synergistic Therapeutic Effects with Paclitaxel in Ovarian CancerInternational Journal of Stem CellsFebruary 28, 2028Suji Jeong, Seho Cha, Haengseok Song, Heesoon Chang, Sung Hun Na, Seok Ho Hong, Mira Parkconditions. CCK8 assay Cell viability was assessed using Cell Counting Kit-8 (WST-8/CCK8) (Abcam; #ab228554) according to the manufacturer’s instructions. A ready-to-use CCK8 solution was added to the cell culture medium

(2) Quinoa and Peanut Extracts as Alternatives to Animal-Derived Extracellular Matrix Proteins in Cultured Meat ProductionJ Food SciSeptember 27, 2026Dominik Mundt, Kuo-Hui Chiu, Stefan Schillberg, Che Julius Ngwausing the Cell Counting Kit‐8 (CCK‐8) assay according to the manufacturer's instructions (Abcam, UK; ab228554). 2.4 Cell‐Free Protein Expression For cell‐free expression of the RGD‐containing proteins, the bifunctional

(3) Single-cell transcriptomics reveals that tenocyte-derived MIF contributes to tendon injury pathogenesisiScienceSeptember 18, 2026Cheuk Hin Kot, Xiaojie Xu, Peifeng Hong, Xu Liang, Gerun Chen, Zebin Macommercial assays,Critical commercial assays,Critical commercial assays ,, CCK-8 assay kit,Abcam,Cat#ab228554 ,, Deposited data,Deposited data,Deposited data ,, scRNA-seq raw data and analyzed data,Huang et

(4) Single-cell transcriptomics reveals critical role of CD74 in bone marrow-derived mesenchymal stem cellsStem Cells Transl MedSeptember 18, 2026Qinqin Xu, Carson Curtis, Jiahao Zhang, Arjun Verma, Aaron J Sheppard, Jeffrey Deng, Hicham Drissi, Shane Barton, Patrick Massey, Yufeng Donggrowth rate Cell growth rate was measured by cell counting in a hemocytometer and WST-8/CCK8 assay (ab228554, Abcam).13 Cells were plated 5 × 103 cells per well in 96-well plates or 5 000-20 000 cells per well

(5) Loss of RanBP9 cooperates with p53 deficiency to promote sarcomagenesisFront OncolSeptember 3, 2026Brianna C. Gonga-Cavé, Gabriel Onea, Xu Wang, Sean P. Cregan, Michael B. Boffa, Patti K. Kiser, Caroline Schild-Poultertissue culture plates. Cell density was measured at indicated time points using the Cell Counting Kit 8 (CCK-8; Ab228554, Abcam) according to the manufacture’s protocol. Briefly, 10µL of WST-8 solution was added to each well

(6) Acute TGFα drives OA-associated transcriptional and metabolic remodelling in primary chondrocytesOsteoarthr Cartil OpenSeptember 2, 2026Emily L. White, Emily A. Day, C. Thomas Appleton, Matthew W. Grol, Frank BeierAgilent XF Seahorse Analytics. 2.7 Confirmation of cell viability The Cell Counting Kit 8 (CCK-8; Abcam ab228554) was used to assess cell viability. Isolated iMACs were seeded in triplicate (7.5 × 104 cells/cm2) in

(7) HSPA8 orchestrates SNARE complex assembly to drive extracellular vesicle-mediated spread of p-tau217 in Alzheimer's diseaseTransl NeurodegenerAugust 27, 2026Bin Xu, Zhen Guo, Jun Chen, Yazhou Xie, Pan Wang, Xiao Xiong, Jiayi Yu, Zhi Xu, Yuan Fu, Zhuoqing Lan, et al.were recorded. Cell viability assay Cell viability was assessed using a CCK-8 assay kit (Abcam, Cat# ab228554, Cambridge, UK) in accordance with the manufacturer’s instructions. Cells were plated in 96-well plates,

(8) N-Myristoyltransferase 2 Is Regulated by Polyphosphate-Driven Lysine InteractionsBiomoleculesJuly 27, 2026Isabella Martins, Kaia Bailey, Anni Ge, Ethan Belrose, Xiaolong Yang, Zongchao JiaMetabolic activity was assessed by adding 10 µL of metabolic activity reagent (Abcam, Waltham, MA, USA, Cat#AB228554) per 100 µL of culture medium, followed by incubation for 4 h at 37 °C. Absorbance was subsequently

(9) MMF inhibits poxvirus infection by disrupting IMPDH2 interaction with USP5 and inducing its rod-and-ring assembliesVirologica SinicaJuly 23, 2026Qian Sun, Kesen Liu, Wandi Cao, Chengyue Wu, Hanhua Zhang, Xingya Wang, Chen Peng, Jie Sun, Anbing Zhang, Zhuo Zhou, et al.the EC50 was calculated. Cell viability was assessed using Cell Counting Kit-8 (CCK-8) assay (Abcam, #ab228554). HeLa cells were treated with candidate drugs at different concentrations for 24 h. Afterward, cell

(10) The Cuproptosis-Associated AL139023.1/miR-139-5p/ELOVL5 ceRNA Axis Regulates Proliferation, Migration, and Cell Cycle in Esophageal Squamous Cell CarcinomaCancer Manag ResJuly 18, 2026Kai Liang, Yun Gao, Xiangyu Zhang, Jin Yang103/well in 96-well plates, incubated overnight (37°C, 5% CO2), and treated with 10 µL CCK-8 reagent (ab228554, Abcam, USA) for 3 h. Absorbance at 450 nm was measured on a Molecular Devices microplate reader and

(11) Spray-Pyrolyzed Hollow and Yolk–Shell CeO 2 Nanocarriers with Tunable Structure for Redox-Responsive Delivery and Gene Rescue ApplicationsACS Applied Materials & InterfacesJuly 15, 2026Jalal Poostforooshan, Cléa Chesneau, Laurent Michely, Madara Dias Wickramanayaka, Iwona Pranke, Patrick Balaguer, Abdelhay Boulahtouf, Fabrice Lejeune, Benedikt Eberhardt, Alexandre Hinzpeter, et al.photocolorimetric analysis using the Cell Counting Kit 8 (WST-8) according to the manufacturer’s guidelines (Abcam, Ab228554). This involved diluting CCK8 in DMEM with FBS (1:10 ratio) and adding 100 μL to each well. The cells

(12) Kaempferol Attenuates Spaceflight-Associated Knee Cartilage Degradation by Targeting NOX4-Mediated Mitochondrial DysfunctionAdv Sci (Weinh)July 9, 2026Yuesong Yin, Ruiling Xu, Meagan J. Makarczyk, Jia-Jun Liu, Silvia Liu, Matthew Z. Shi, Roberta Di Carlo, Michael F. Almeida, Alex Garris, Sarah Day, et al.graded Kaempferol concentrations, and viability was measured at days 0, 1, 3, and 7 using CCK8 (Abcam, ab228554). 5.15 SiRNA Transfection Human primary chondrocytes (∼70% confluence) were transfected with siRNA

(13) Integrative serum metabolite prioritization and functional screening identify N-acetyl-L-glutamine as a protective candidate in premature ovarian insufficiencyFront GenetJuly 2, 2026Xinyue Zhang, Chen Chen, Xiaolan Zhuwashed with phosphate-buffered saline (PBS). Fresh medium containing 10% Cell Counting Kit-8 reagent (Ab228554, Abcam) was then added to each well. After incubation for 2 h at 37 °C in the dark, absorbance at 450

(14) Arsenic exposure promotes retinal pigment epithelium dysfunction and oxidative stress via NF-κB pathwayScientific ReportsJune 8, 2026Lan Zhong, Yumei Tao, Liming Taoand cytotoxicity assessment Cell viability was assessed using a Cell Counting Kit-8 (CCK-8, Abcam, ab228554). ARPE-19 cells were seeded into 96-well plates and treated as described. Following treatment, CCK-8

(15) Hyperoside protects against poly-GR-mediated neurodegeneration via regulation of mitochondrial fission and oxidative stress in C9orf72-associated ALSChin MedJune 4, 2026Wen-Chi Hsieh, Chun-Yu Lin, Hsuan-Cheng Wu, Eddie Feng-Ju Weng, Shao-Ming Wangincubator for the indicated periods. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8; ab228554, Abcam). Diluted CCK-8 reagent was added to each well and incubated for 1 h at 37 °C, after which absorbance

(16) Exposure to high doses of tyre antioxidant 6PPD causes senescence to induce unexplained miscarriage by suppressing BAZ1B-mediated ubiquitination degradation of P21EBioMedicineJune 1, 2026Waiping Zhang, Yan Liang, Yi Sun, Junchang Wang, Wenxin Huang, Shuaishuai Xing, Zhihong Zhang, Yanbing Lin, Liqin Zeng, Qiong Lei, et al.Western blotting. Cell viability Cell viability was evaluated using a Cell Counting Kit-8 (CCK8, ab228554, Abcam, Cambridge, UK). Cells (5 × 103 cells per well, three replicates per group) were seeded in 96-well

(17) Epigenetic brain reprogramming rejuvenates neuro-immune circuits to reverse Alzheimer’s disease pathology and systemic bone lossJournal of NeuroinflammationMay 28, 2026Mei-Dan Wan, Xi-Xi Liu, Teng-Fei Wan, Yi-Wei Liu, Ya-Ling Jiang, Jing-Tao Zou, Bin Jiao, Qian-Qian Liu, Ling Jin, Ran Duan, et al.without AAV-OSK pretreatment. Cell proliferation was assessed after 48 h using the CCK-8 assay (Abcam, ab228554). Flow cytometry Apoptosis in HT22 cells was assessed by flow cytometry following CCCP treatment.

(18) A MUC1-ALIX complex regulates extracellular vesicle cargo loading with activated SRC to enhance pancreatic cancer progressionCancer LettersMay 28, 2026Ying Huang, Kristine V. Hoagstrom, Clara S. Mundry, Tuo Hu, Heather C. Jensen-Smith, Jacob Wragge, Phong Le, Amina Baniya, Kyle L. McAndrews, Thomas C. Caffrey, et al.Lipofectamine 3000,ThermoFisher,Cat#: L3000015 APC/Cy7 Conjugation Kit,Abcam,Cat#: ab102859 Cell Counting Kit 8,Abcam,Cat#: ab228554 CellTiter-Glo® Luminescent Cell Viability Assay,Promega,Cat#: G7572 Cy5™ Mono-Reactive Dye,Amersham,Cat#:

(19) Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expressionNucleic Acids ResMay 26, 2026Zhiyun Yang, Zongchao Jiaobjective or Leica Mica Microhub confocal microscope. WST-8 assay WST-8 kit was purchased from Abcam (#ab228554), and assay was conducted according to the manufacturer’s instructions. Briefly, 1.5 × 104 cells/well

(20) Endothelial Drp1 integrates VEGF-induced redox signaling with glycolysis through cysteine oxidation to drive angiogenesisNature CommunicationsMay 20, 2026Sheela Nagarkoti, Young-Mee Kim, Archita Das, Dipankar Ash, Eric A. Vitriol, Tracy-Ann Read, Varadarajan Sudhahar, Md. Selim Hossain, Shikha Yadav, Stephanie Kelley Spears, et al.Cell viability was measured using CCK8 assay. For CCK8 assay, Cell Counting Kit 8 (WST-8/CCK8) (Abcam, ab228554) was used. CCK-8 solution (CCK8: medium, 1:10) was added into each dish and incubated in the dark at

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(1)

4/19/2023Great Kit for Cell Viability and More Seth TompkinsThis kit is marketed as a way to measure cell viability. This is true, but it can do so much more. The assay works by measuring active mitochondria (more mitochondria, the more the dye is reduced). So... Read Review
(1)

10/4/2022Good Cell Viability Assay Kit Yashavantha VishweshwaraiahWe used this kit for cell viability microplate assay using various cell lines and expression conditions. We got consistent and reproducible results.

Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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