Cell Counting Kit 8 (WST-8 / CCK8) ab228554 from Abcam

Product Specs
ItemCell Counting Kit 8 (WST-8 / CCK8)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228554
Sample TypeAdherent cells, Suspension cells
Quantity1000 Test, 5000 Tests

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) hiPSC-Derived M1 Macrophages Exhibit Synergistic Therapeutic Effects with Paclitaxel in Ovarian CancerInternational Journal of Stem CellsFebruary 28, 2028Suji Jeong, Seho Cha, Haengseok Song, Heesoon Chang, Sung Hun Na, Seok Ho Hong, Mira Parkconditions. CCK8 assay Cell viability was assessed using Cell Counting Kit-8 (WST-8/CCK8) (Abcam; #ab228554) according to the manufacturer’s instructions. A ready-to-use CCK8 solution was added to the cell culture medium

(2) Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapyFront ImmunolApril 16, 2026Xuanyou Liu, Iris Kemler, David Dingliwith results reported in relative light units. In parallel, the cell counting kit-8 (CCK8, Abcam, Cat #ab228554) was used to assess the proliferation and viability of cells lacking luciferase expression, according

(3) Single-Cell Analysis Delineates Glutathione Metabolism-Related Gene Signatures in the Glioblastoma Microenvironment and Identifies GSTA4 as a Regulator of Malignant BehaviorsInt J GenomicsApril 13, 2026Baozhi Feng, Jie Wang, Wei Shang, Huihao Ma, Yan Fu, Hongtao Lv, Yinghui Xu, Bin Dongconditions (5% CO2, 37°C). At 24, 48, and 72 h postseeding, cell viability was assessed using a CCK‐8 assay (ab228554, Abcam). A working solution containing 10% CCK‐8 reagent in culture medium was prepared and incubated

(4) Protein Expression Analysis and Functional Characterization of Sorcin in Gallbladder CancerCellsApril 12, 2026Vaishali Jain, Neeraj Saklani, Srishti Kawatra, Puja Sakhuja, Surbhi Goyal, Anil Kumar Agarwal, Parveen Kumar, Fouzia Siraj, Poonam GautamAnalysis Cancer cell proliferation was evaluated using the Cell Counting Kit (CCK)-8 assay (cat. no. ab228554; Abcam, Cambridge, UK). NOZ cells were seeded in 24-well plates (5.0 × 103 cells/well) and transfected

(5) Phillyrin Modulates AMPK-Associated Cellular Responses and Migration in PC3 Prostate Cancer CellsJ ToxicolApril 11, 2026Cheng-Hsin Lu, Chun-Hsien Wu, Pei-Fang Hsieh, Richard Chen-Yu Wu, Hsing-Chia Mai, Wei-Lun Huang, Sih-Han Chen, Chien-Ming Lai, Victor C. Lin, Chiang-Ting Wangcontrol group were cultivated in a standard culture medium without treatment. 2.3. CCK8 Assay CCK8 (cat. No. ab228554; Abcam, Cambridge, UK) was used to assess cell viability. Before treatment, PC3 cells were seeded in

(6) Endogenous and environmental factors that induce DNA replication defects and genomic instability in ER-negative heterozygous BRCA1 cellsScientific ReportsMarch 27, 2026Madhura Deshpande, Theodore Paniza, Rebecca Brown, Kate Heslin, Nitya Patel, Advaitha Madireddy, Zev Rosenwaks, Jeannine Gerhardtequivalent amount of DMSO. After incubation, the cell viability was assessed using CCK-8 kit (Abcam, #ab228554). WST-8 reagent provided was added to each well, and plates were incubated for 4 h at 37 °C. The plates

(7) USP7 Drives Atherosclerosis by Promoting Ferroptosis in Vascular Endothelial Cells via the KIAA1429/NEAT1/CTCF AxisBiological Procedures OnlineMarch 13, 2026Bo Dong, Lu Kou, Jing-Yu Yang, Yang Li, Ning Yangox-LDL for 24 h. Cell Counting Kit-8 (CCK-8) Assay Cell viability was detected using the CCK-8 method (ab228554, Abcam). The collected HUVECs were inoculated into 96-well plates at 4 × 103 cells/well, and 10 µL of

(8) Sigma-1 receptor regulates HIV-1 and methamphetamine-induced endothelial/pericyte barrier impairment via strain-specific inflammatory responses and mitochondrial dysregulationJournal of NeuroinflammationMarch 6, 2026Nikolai Fattakhov, Silvia Torices, Sarah Becker, Joelle-Ann Joseph, Sarah Schmidlin, Alex Ngo, Adesuwa Okoro, Oandy Naranjo, Michal ToborekCell viability assay HBVP viability was assessed using the Cell Counting Kit 8 (CCK-8) assay (Abcam, Cat# ab228554) according to the manufacturer’s protocol. Briefly, 10 µL of CCK-8 reagent was added to each well of

(9) Production and characterization of rNGFSP: a recombinant fusion immunogen eliciting dual anti-NGF and anti-Substance P therapeutic antibodies for Degenerative Joint DiseaseBiotechnology ReportsMarch 1, 2026Valentina Varela, Monique Costa, Cecilia Maciel, Joaquín Barbeito, Exequiel E. Barrera, Erica Gutierre, Agustín Correa, Melania Elgue, Sebastián Carrasco, Magdalena Domínguez Larrosa, et al.hyperimmune dog sera, and appropriate controls. After 48 h incubation, 20 μL CCK-8 reagent (Abcam, ab228554) was added to estimate cell number, followed by 24 h incubation. Absorbance at 460 nm was measured using

(10) Integration proteomics analysis to identify AMPK as key target pathways of TCM formula for high fat diet induced obesity in miceJournal of Traditional and Complementary MedicineMarch 1, 2026Yu-Ju Chen, De-Shan Ning, Ching-Chiung Wang, Hong-Wei Zhao, Kun-Teng Wang, Ming-Chung Lee, Wan Chun Chiu, Chiu-Li Yeh, John Louie Jacinto Dela Vega, Chia-Jung Lee50, 100, and 200 μg/ml 24 h. Cell viability was determined using cell counting kit-8 (CCK-8) assay (ab228554, Abcam, Burlingame, CA, USA). CCK-8 solution was added to each well for 3 h, following which cell viability

(11) New N-Heterocyclic Carbene Gold and Platinum Complexes with 1,3-Dialkyl-4-anisyl-5-(4-chlorophenyl)imidazol-2-ylidene Ligands for the Treatment of Esophageal AdenocarcinomaInternational Journal of Molecular SciencesFebruary 21, 2026Hindole Ghosh, Tobias Rehm, Sangita Bhattacharyya, Miru Lee, Dileepkumar Veeragoni, Rainer Schobert, Bernhard Biersack, Prasad Dandawateand cell viability was assessed using the hexosaminidase enzymatic assay [44]. A CCK-8 assay (Abcam#ab228554, Cambridge, MA, USA) was performed on THP-1 cells. Briefly, 5000 cells were seeded in each well of a

(12) Dysbiosis of oral and gut microbiomes characterized by elevated Lactococcus in a mouse model of oral squamous cell carcinomanpj Biofilms and MicrobiomesFebruary 13, 2026Euon Jung Tak, Beom-Jin Goo, Jae-Yun Lee, Jeong Eun Han, Yun-Seok Jeong, Hae-In Joe, Hojun Sung, Hyun Sik Kim, Jin-Woo Bae(v/v). After 24 h of incubation, supernatants were aspirated, and 100 µL of WST-8 reagent (Abcam, cat# ab228554) was added to each well to assess cell viability. Each condition was assessed in at least five technical

(13) Baicalin as a Regulator of Peroxisome Proliferator-Activated Receptor Gamma: Alleviating Sepsis-Induced Liver Injury by Inhibiting the Cluster of Differentiation 14/Nuclear Factor Kappa B Signaling PathwayImmun Inflamm DisFebruary 12, 2026Hui Wang, Mengmeng Guo, Xueling Zeng, Yuqi Hu, Jiawei Ma, Yufei Changtreatment with 100 µM BA followed by 0.5 µg/mL LPS for 24 h). 2.4 Cell Counting Kit 8 (CCK8) Assay CCK8 (ab228554, Abcam, UK) was used to assess cell viability. In short, AML12 cells were seeded in 96‐well plates (FPT011,

(14) Desmoglein 2 (DSG2)-knockout human respiratory epithelial cell model to study species B adenovirus receptor usageVirologica SinicaFebruary 10, 2026Nora Bahlmann, Montaha Alshawabkeh, Raphael Tsoukas, Katrin Schröer, Sebastian Schellhorn, Mareike Sieler, Thomas Dittmar, Eric Ehrke-Schulz, Anja Ehrhardt, Wenli ZhangCell viability assay To monitor the cell proliferation after receptor knockout, a CCK-8 assay was performed (ab228554, Abcam, UK). The cells seeded in 96 well plates were measured at different time points. The absorbance

(15) Residuals of Chemical Cleaning Agents Impair Peri-Implant Cell Viability: An in Vitro StudyACS Biomaterials Science & EngineeringFebruary 9, 2026Qiang Wang, Håvard Jostein Haugen, Dirk Linke, Ståle Petter Lyngstadaas, Qianli MaCCK8 Assay Cell proliferative viability was measured using the Cell Counting Kit-8 (CCK-8; Abcam, ab228554). Cells were seeded in 96-well plates at a density of 7500 cells per well (≈2.3 × 104 cells/cm2) and

(16) Optoacoustically augmented magnetic guidewire for radiation-free minimally invasive therapiesSci AdvFebruary 6, 2026Fan Wang, Xianqiang Bao, Erdost Yildiz, Yan Yu, Xosé Luís Deán-Ben, Wenbin Kang, Shuaizhong Zhang, Devin Sheehan, Ren Hao Soon, Jelena Zinnanti, et al.cells were used as controls. WST-8 cell counting assay Meanwhile, the WST-8 cell counting kit (Abcam, ab228554) was used to verify the photo effect according to the user manual. Briefly, 1/10 volume of WST-8 solution

(17) Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradationEBioMedicineFebruary 5, 2026Yi Sun (孙义), Yanbing Lin (林晏冰), Shuaishuai Xing (幸帅帅), Wenxin Huang (黄文欣), Yanxin Wang, Geng Guo, Haijun Yan, Depeng Zhao, Wenli Cheng, Zhengzhong Wu, et al.validated by RT-qPCR and WB. Cell viability Cell viability was evaluated using Cell Counting Kit-8 (CCK8, ab228554, Abcam, Cambridge, UK). Cells (5 × 103 cells per well, three replicates for each group) were seeded

(18) Integrative functional genomics analysis identifies pleiotropic genes for vascular diseasesNature CommunicationsFebruary 5, 2026Charles U. Solomon, David G. McVey, Catherine Andreadi, Peng Gong, Lenka Turner, Dedrick S. S. Song, Heming Zhang, Dominic P. Lee, Elisavet Karamanavi, Wei Yang, et al.post-transfection, cells were subjected to a proliferation assay with the use of Cell Counting Kit-8 (Abcam, ab228554). Human aortic smooth muscle cells (Lonza, CC-2571) were transfected with either SMARCA4 siRNA (ThermoFisher

(19) Shelterin Component TPP1 Drives Tumor Progression and Predicts Poor Prognosis in Hepatocellular CarcinomaBiomedicinesFebruary 4, 2026Jung Eun Jang, Hye Seon Kim, Jin Seoub Kim, Jae Mo Han, Hee Sun Cho, Kwon Yong Tak, Ji Won Han, Pil Soo Sung, Si Hyun Bae, Jeong Won JangProliferation Assay Cell viability was evaluated using a CCK-8 assay (Cell Counting Kit-8, Abcam, ab228554) according to the manufacturer’s protocol. Cells were seeded into a 96-well plate at a uniform density

(20) Panobinostat Potentiates the Antitumor Efficacy of 5-Fluorouracil in Gastric Cancer by Suppressing Thymidylate Synthase ExpressionInt J Mol SciFebruary 3, 2026Sooyeon Park, Nayeon Kim, Changwon Yangsimultaneously. 4.2. CCK8 Assay Cell viability was assessed using the Cell Counting Kit8 (CCK8) (ab228554, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Cells were seeded into 96-well

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(1)

4/19/2023Great Kit for Cell Viability and More Seth TompkinsThis kit is marketed as a way to measure cell viability. This is true, but it can do so much more. The assay works by measuring active mitochondria (more mitochondria, the more the dye is reduced). So... Read Review
(1)

10/4/2022Good Cell Viability Assay Kit Yashavantha VishweshwaraiahWe used this kit for cell viability microplate assay using various cell lines and expression conditions. We got consistent and reproducible results.

Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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