Smad3 (C67H9) Rabbit mAb for Western Blot

January 31, 2023

BlueskyReddit
Houston Methodist Research Institute

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Company:

Cell Signaling Technology

Product Name:

Smad3 (C67H9) Rabbit mAb

Catalog Number:

9523S

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: SMAD3 Antibodies

I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer. I was interested to activated the pSMAD pathway related to ECM deposition.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer. I was interested to activated the pSMAD pathway related to ECM deposition.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Highly recommended.

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