Smad3 (C67H9) Rabbit mAb 9523S from Cell Signaling Technology

Product Specs
ItemSmad3 (C67H9) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number9523S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP, ChIP, ChIP-seq
ReactivityH, M, R, Mk
TargetSmad3
HostRabbit
NCBI Gene AliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
ClonalityMonoclonal
NCBI Full Gene NameSMAD family member 3
Research AreaDevelopmental Biology
IsotypeIgG
CloneC67H9
Molecular Weight52

Questions and Answers

Can this antibody be used to study the role of SMAD3 in TGF-β signaling?

Yes, the Smad3 (C67H9) Rabbit mAb has been shown to be effective in applications related to TGF-β signaling. For instance, in a study, targeted dephosphorylation of SMAD3 was utilized to impede TGF-β-induced transcriptional responses, demonstrating the antibody's relevance in investigating SMAD3's role in this pathway ().

Is this antibody suitable for use in immunoprecipitation experiments?

Yes, the Smad3 (C67H9) Rabbit mAb is applicable for immunoprecipitation (IP). It has been cited in various studies where it was used to pull down SMAD3 protein, allowing for further analysis of its interactions and modifications ().

What species can this antibody detect, and is it effective for human samples?

The Smad3 (C67H9) Rabbit mAb is reactive with multiple species, including human, mouse, rat, and non-human primates. It has been effectively used in studies involving human samples, confirming its utility in diverse biological contexts ().

Has this antibody been utilized in chromatin immunoprecipitation assays?

Yes, the Smad3 (C67H9) Rabbit mAb has been employed in chromatin immunoprecipitation (ChIP) assays. It was used to investigate the recruitment of the SMAD complex to specific enhancer regions, highlighting its role in transcriptional regulation in response to TGF-β signaling ().

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Hydrogen sulfide ameliorates peritoneal fibrosis: inhibition of high mobility group box-1 expression to block the activation of the transforming growth factor-beta/Smad3 pathwayMedical Gas ResearchJune 1, 2026Lulu Liu, Weiyan Huang, Jiao Ming, Fei Cai, Haiyan Wang, Xueli Lai, Zhiyong GuoUSA)",HPA001240,AB_1079408 HMGB1,Rabbit,1:1000,CST,3935,AB_2295241 FN1,Rabbit,1:1000,CST,63779,AB_2637836 TGF-βΙ,Rabbit,1:1000,Abways,CY6608,AB_3663010 SMAD3,Rabbit,1:1000,CST,9523,AB_2193182 P-SMAD3,Rabbit,1:1000,CST,9520,AB_2193207 Information on the antibodies used 3-MST:

(2) TGIF2-mediated HMGB3 overexpression promotes esophageal squamous cell carcinoma proliferation and metastasis through TLR3/TGF-β signalingGenes & DiseasesMay 1, 2026Liaoran Niu, Wanli Yang, Wei Zhou, Lili Duan, Qi Wang, Xiaoqian Wang, Yiding Li, Chengchao Xu, Yujie Zhang, Jinqiang Liu, et al.Cell Signaling Technology, 1:1000), anti-p-SMAD2 (#3108; Cell Signaling Technology, 1:1000), anti-SMAD3 (#9523; Cell Signaling Technology, 1:1000), anti-p-SMAD3 (#9520; Cell Signaling Technology, 1:1000), anti-extracellular

(3) ATG7 vs. ATG5: Distinct autophagy pathways shaping TGF-β signaling and endothelial functionPLoS OneApril 30, 2026Aman Singh, Bharatsinai Peddi, Kriti S. Bhatt, Hien C. Nguyen, Krishna K. Singh#12994), ATG7 (Cell Signalling, Cat #8558), p21 (Cell Signaling # 2947S), SMAD3 (Cell Signalling, Cat #9523), SMAD5 (Cell Signalling, Cat #12534), LC3 (Proteintech, Cat #14600–1-AP) and GAPDH (Cell Signaling

(4) Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic woundsCell Reports MedicineApril 21, 2026Li Lu, Yuan Xiong, Jiewen Liao, Juan Zhou, Guangji Wang, Yating Qin, Shengming Zhang, Yanzhi Zhao, Xiaodan Zhong, Mengwen Wang, et al.,Cell Signaling Technology,Cat#3033; RRID: AB_331284 Rabbit monoclonal anti-Smad3,Cell Signaling Technology,Cat#9523; RRID: AB_2193182 Rabbit monoclonal anti-STAT1,Cell Signaling Technology,Cat#14994; RRID: AB_2737027 Rabbit

(5) Proliferation And Apoptosis Adaptor Protein 15 (PEA15), a potential oncogenic regulator of VHL and HIF1A identified through proteomic analysis in hepatocellular carcinomaCancer CommunicationsApril 8, 2026Yun Seong Jeong, Yun Seong Jeong, Ji-Hyun Shin, Soo Mi Sohn, Sun Young Sohn, Sun Young Yim, Ji-Hoon Shim, Sung Hwan Shim, Sung Hwan Chun, Sunyoung S. Chun, et al.3 (STAT3) (1:1,000, #9139, Cell Signaling Technology), anti-SMAD family member 3 (SMAD3) (1:1,000, #9523, Cell Signaling Technology), and anti-vascular endothelial growth factor A (VEGFA) (1:1,000, #50661,

(6) ZNF384-Driven Fibulin-1 Exacerbates Vascular Stiffness via TGF-β/Smad3-Mediated Senescence and FibrosisFASEB JMarch 31, 2026Dan Yan, Tianyi Ji, Xiaolu Liang, Mandi Luo, Yi Huang, Pengcheng Luo, Zhen Yang, Le Zhang, Tao Li, Yong Ping Bai, et al.(ab270993, Abcam, 1:1000), Collagen III (ab184993, Abcam, 1:1000), TGF‐β (#3709, CST, 1:1000), Samd3 (#9523, CST, 1:1000), P‐Samd3 (ab52903, Abcam, 1:1000) or GAPDH (#2118, CST, 1:5000). After washing with TBST,

(7) Disrupting the TGF-β-regulated epithelial-mesenchymal transition, apoptotic and autophagic phenotypes of 3D glioblastoma spheroids via glycolytic inhibitionExploration of Targeted Anti-tumor TherapyMarch 30, 2026Maellis Payet-Desruisseaux, Alain Zgheib, Bogdan Alexandru Danalache, Michel Desjarlais, Borhane AnnabiS), phospho-AKT Ser473 (12604, 4060 S), Snail (C15D3, 3879 S), Smad2 (D43B4, 5339 S), Smad3 (C67H9, 9523 S), phospho-Smad2 Ser465/467 (138D4, 3108 S), and phospho-Smad3 Ser423/425 (C25A9, 9520 S) were from

(8) Polyethylene terephthalate microplastics impair erectile function through macrophage mediated cGAS-STING ferroptosisiScienceMarch 20, 2026Siyu Liu, Wenhao Wang, Yijun Zhang, Shufeng Li, Haoyi Jia, Shiyun Liu, Lei Wu, Pingnan Dou, Jianying Li, Chenyi Jiang, et al.GPX4 (Abcam, ab125066, 1:1000), E-cadherin (CST #3195, 1:1000), Smad2 (CST #5339, 1:1000), Smad3 (CST #9523, 1:1000), p-Smad2 (CST #3108, 1:1000), p-Smad3 (CST #9520, 1:1000), CD31 (Abcam, ab28364, 1:1000), and

(9) Pelargonidin inhibits isoproterenol induced myocardial fibrosis via regulating transforming growth factor-β/Smad2/3 signaling and Th2 cytokines in micePhysiological ResearchMarch 10, 2026Juanhua Zhao, Linping Dong, Long Yaoantibodies including TGF-β1 (Cat# 3711), Smad2 (Cat# 3103), phospho-Smad2 (Cat# 3108), Smad3 (Cat# 9523), phospho-Smad3 (Cat# 9520), α-SMA (Cat# 19245), COL3A1 (Cat# 22734), FN1 (Cat# 26836), and β-actin

(10) Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosisExperimental & Molecular MedicineMarch 4, 2026Hui Jae Lim, Yong Kwon Han, Mi Ra Noh, You Ri Park, Se Young Jang, Joshua H. Lipschutz, Kwon Moo ParkExoc7 (cat. no. 28666, Cell Signaling Technology), p-Smad3 (cat. no. ab52903, Abcam), Smad3 (cat. no. 9523, Cell Signaling Technology), β-catenin (cat. no. 8480, Cell Signaling Technology), alpha-smooth muscle

(11) Cell type–dependent role of transforming growth factor-β signaling on postnatal neural stem cell proliferation and migrationNeural Regeneration ResearchMarch 1, 2026Kierra Ware, Joshua Peter, Lucas McClain, Yu LuoSmad 2,Cell signaling,mAb# 5339 p-Smad 3,Cell signaling,mAb# 9520 Total Smad 3,Cell signaling,mAb# 9523 Critical commercial assays and components,Critical commercial assays and components,Critical commercial

(12) LncRNA RMST knockout inhibits fibrosis by down-regulating Smad3 during mouse skin wound healingBiochemistry and Biophysics ReportsMarch 1, 2026Zhong Zhou, Xulong Huang, Chaohang Chen, Tao Zou, Yifan Hai, Guokai Dong, Shanshan Li(sc293182),1:500,SANTA CRUZ α-SMA (67735-1-Ig),1:50000,Proteintech TGF-β1 (21898-1-AP),1:2000,Proteintech Smad3 (9523T),1:1000,CST β-actin (AP0060),1:20000,Bioworld TNF-α (AF7014),1:500,Affinity Il-1β (16806-1-AP),1:2000,Proteintech Antibodies

(13) Reduced cyclin D3 expression in erythroid cells protects against malariaNatureFebruary 18, 2026Maria Giuseppina Marini, Maura Mingoia, Maristella Steri, Ioannis Tsamesidis, Maria Laura Idda, Alessia Manca, Cristina D’Avino, Francesca Virdis, Valeria Lodde, Antonella Mulas, et al.immunoprecipitation and 1:1,000 for western blot) supplied by Cell Signaling Technology, clone C67H9, 9523; anti-human GATA1 (GATA1, 10 µg in 100 µl) supplied by Abcam, ab11852; and normal rabbit IgG, (IgG,

(14) Nur77 Regulates the Phosphorylation of Smad3, Thereby Influencing Skeletal Muscle Fibrosis Caused by ObesityFASEB JFebruary 15, 2026Na Li, Tingting Tian, Qihe Zhao, Difei Wanganti‐Collagen III a1 (1:1000, Proteintech, 22 734‐1‐AP), Rabbit anti‐Smad3 (1:1000, Cell Signaling Technology, 9523), Rabbit anti‐p‐Smad3 (1:1000, Cell Signaling Technology, 9520), Rabbit anti‐TGF‐β1 (1:1000, Abcam,

(15) Forsythiaside A inhibits the progression of intrahepatic cholangiocarcinoma partially via suppressing oxidative stress-induced hepatic stellate cell activation and macrophage polarizationBMC BiotechnologyFebruary 10, 2026Shiyu Zhang, Fan Du, Ye Liao, Chuyu Zhang, Meixia ZhangCell signaling technology), anti-p-Smad2 antibody (1:1000; #18338, Cell signaling technology), anti-Smad3 antibody (1:1000; #9523, Cell signaling technology), anti-p-Smad3 antibody (1:1000; #9520, Cell signaling technology), anti-hypoxia-inducible

(16) Bioabsorbable Endovascular Adhesive Tape (BEAT) for Improving Vascular RegenerationAdvanced ScienceFebruary 10, 2026Jiarong Wang, Jing Wang, Xinyi Li, Bo Yu, Yiduo Chen, Yirong Guo, Honglin Qian, Meng Hu, Haoyang Liu, Wenhui Liu, et al.protein expression levels of collagen‐I (Col‐I, CST 72026), α‐smooth muscle actin (α‐SMA, Abcam ab7817), Smad3 (CST 9523), and phospho‐Smad3 (p‐Smad3, CST 9520) were evaluated by Western blot. To evaluate the function of

(17) Curcumin pretreatment enhances the capacity of BMSC exosomes to attenuate renal ischemia-reperfusion injury by ferroptosis suppression via miR-16-5p/Smad3/Mb axisStem Cell Research & TherapyFebruary 9, 2026Bingyi Chen, Jinxing Chen, Zekun Shen, Shuang Liu, Yifan Mei, Hui Cai, Kai Li, Ziyang Peng, Liwei Zhang, Weiyi Wang, et al.was aliquoted as Input. The remaining samples were incubated overnight at 4 °C with Smad3 antibody (#9523, CST) under gentle rotation, using IgG antibody as a negative control. Following all washing steps,

(18) Endothelium-Treg Communication Through Extracellular Vesicle Transfer Exacerbates Acute Respiratory Distress SyndromeJ Extracell VesiclesFebruary 8, 2026Xu Liu, Wei Huang, Xiwen Zhang, Shiming Li, Haofei Wang, Zongsheng Wu, Shuangfeng Zi, Lu Wang, Ling Liu, Yi Yang, et al.USA); smad2 rabbit mAb (5339, CST, USA); phosphor‐smad3 rabbit mAb (9520, CST, USA); smad3 rabbit mAb (9523, CST, USA); phosphor‐STAT5 rabbit mAb (25656, CST, USA); STAT5 rabbit mAb (94205, CST, USA); phosphor‐STAT3

(19) Serpine1 Regulates the Enhanced Inhibitory Effect of CHIR99021 Combined with Fibroblast Growth Factor 2 on Myocardial Fibrosis After Myocardial Infarction in MiceInternational Journal of Molecular SciencesFebruary 7, 2026Yangyang Jia, Xiangqin Tian, Mengyu Wei, Pingping Xu, Jikui Wang, Yaping Xu, Changye Sun, Zhikun Guoanti-Smad2 (ET1604-22, HuaBio, 1:5000, China), anti-p-Smad2 (ET1612-32, HuaBio, 1:5000, China), anti-Smad3 (#9523, CST, 1:1000, Danvers, MA, USA), anti-p-Smad3 (#9520, CST, 1:1000, Danvers, MA, USA), anti-FAK (ET1602-25,

(20) IFN-γ and TNF-α Impair Lung Development by Upregulating SMAD7 to Inhibit TGF-β Signaling Pathway and ECM DysregulationInflammationFebruary 3, 2026Xiaotian Liao, Weiliang Huang, Jianwei Wei, Lu Zhu, Xiaojun Lin, Zitong Mo, Chunhong Jia, Zhiwen Su, Fan WuSMAD2 (Cat#5339, 1:1000), phosphorylated SMAD2 (p-SMAD2, Ser465/467, Cat#18338, 1:1000), SMAD3 (Cat#9523, 1:1000), phosphorylated SMAD3 (p-SMAD3, Ser423/425, Cat#9520, 1:1000), and SMAD4 (Cat#38454, 1:1000)

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1/31/2023Smad3 (C67H9) Rabbit mAb for Western Blot Gherardo BaudoI used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer. I was interested to ... Read Review

Smad3 (C67H9) Rabbit mAb

Smad3 (C67H9) Rabbit mAb from Cell Signaling Technology

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Smad3 (C67H9) Rabbit mAb from Cell Signaling Technology

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