Great Mouse IFN-Gamma Protein

Houston Methodist Research Institute
Nanomedicine
Postdoctoral Researcher

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Company:

R&D Systems

Product Name:

Mouse IFN-gamma Protein

Catalog Number:

485-MI-100/CF

I used this mouse IFN-gamma (IFNɣ) Protein in mice bone-marrow-derived macrophage cells (BMDM). BMDMs were induced to M1 macrophage cells by 10 ng mL−1 IFNɣ and 10 ng mL−1 LPS. It works great, and the stimulations are very stable.

Experimental Design and Results Summary

Application

M1 macrophage cells stimulation

Starting Material

N/A

Tips

Use M-CSF culture BMDM for 7 days and then IFNɣ and LPS to stimulate M2 macrophage cells.

Results Summary

24 h stimulation of BMDM by 10 ng mL−1 IFNɣ and 10 ng mL−1 LPS is good enough to generate M1 macrophage cells. The results are stable and reliable.

DOI or PMID #

N/A

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Summary

The Good

Reliable

The Bad

Expensive

The Bottom Line

This IFNɣ protein works great for M1 macrophage stimulation. Highly recommended.

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