
Diverse protein and peptide sources can supply the needs of biomedical or biochemical research.
These biomolecules can fulfill a variety of roles, such as: growth factors and cytokines for cell culture, active enzymes for biochemical assays, carrier proteins for movement of molecules, inhibitors for blocking pathways, and antigens for use with antibodies.
Blocking peptides, which comprise of synthetically prepared short amino acid segments, can be used in experiments involving antibodies, such as for confirming antibody specificity. Obtaining ready-to-use commercial proteins or peptides can save a great deal of time and effort.
Recombinant proteins can come purified from E. coli, mammalian cells, or even from cell-free methods.
As with other chemical reagents, the suppliers often provide a measure of purity, typically with SDS-PAGE.
Our recombinant protein search tool offers the ability to browse through the entire catalog of proteins and peptides from a variety of suppliers.
Some considerations to keep in mind when choosing to purchase from a supplier include: the species origin of the gene, the source of the purified product, the intended applications, enzymatic activity, lyophilized or reconstituted formats, and any associated tags.
Recommended Reading:
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Peptidomics—the characterization of intact peptides in a biological sample—is fast emerging as an approach to uncover another layer in human health and disease.
Although challenges remain when sampling peptidomes, advances in sample processing, mass spectrometry, and data analysis have enabled the identification of peptide biomarkers for cancer and infectious disease diagnostics.
Innovative methods are supplementing traditional methods
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Similar to kappa and lambda on B cells, TRBC1 and TRBC2 are being used nowadays for evaluating T cell clonality. Although some labs use only TRBC1, we decided to use both because it allows for better resolution of clonal T cells especially when the ...
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The authors were able to identify SARS-CoV-2 B-cell epitopes using peptide microarray using this antibody. The epitope was detected by measuring red fluorescent-labeled anti-human IgG antibody.
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