Recombinant Mouse IFN-gamma Protein, CF 485-MI-100/CF from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Mouse IFN-gamma Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $503.00Supplier Page View Company Product Page
Catalog Number485-MI-100/CF
Size100 ug
ApplicationsBioactivity
FormatLyophilized from a 0.2 µm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP with Trehalose
Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular WeightObserved: 11-12 kDa, reducing conditions
Theoretical: 15.6 kDa
Conjugate/TagUnconjugated
SpeciesMouse
Molecule NameIFN-gamma
Source/Expression SystemE. coli-derived Recombinant Mouse IFN-gamma Protein, CF
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFN-g, Ifg, If2f
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Akt inhibition enhances the antitumor efficacy of immune checkpoint blockades and radiotherapy in a syngeneic breast cancer modelMolecular Therapy OncologyDecember 18, 2025Nawon Park, Seung Hyuck Jeon, Yoomin Kim, Seongmin Kim, In Ah Kim(LPS; 100 ng/mL; Sigma-Aldrich, USA, L6529-1MG) and interferon-γ (IFN-γ; 20 ng/mL; R&D Systems, USA, 485-MI-100/CF), or IL-4 (20 ng/mL; PeproTech, 204-14), respectively, for 24–48 h. Akt inhibitor at different concentrations

(2) CXCL1 augments host defense against Legionella pneumophila through the IL-18–IFN-γ axis and neutrophil homeostasisThe Journal of ImmunologyNovember 1, 2025Ky V Hoang, John Le, Xiaoqian Shan, Samithamby Jeyaseelanin Cxcl1−/− mice, in vivo reconstitution of IFN-γ and IL-18 was performed. Recombinant IFN-γ (Cat# 485-MI-100/CF, R&D System, Minneapolis, MN) or IL-18 (Cat# 9139-IL-050/CF, R&D System) was administered intranasally

(3) An interleukin-27-centered cytokine circuit regulates macrophage and T cell interactions in autoimmune diabetesiScienceOctober 17, 2025Ashley E. Ciecko, Rabia Nabi, Amber Drewek, David M. Schauder, Pavel N. Zakharov, Xiaoxiao Wan, Scott M. Lieberman, Weiguo Cui, Martin J. Hessner, Chien-Wei Lin, et al.,BD Bioscience,Cat#565694 Zombie NIR Fixable Viability Kit,BioLegend,Cat#423105 Recombinant mouse IFN-gamma,R&D SYSTEMs,Cat#485-MI-100/CF Recombinant mouse M-CSF,R&D Systems,Cat#416-ML-010/CF ,, Critical Commercial Assays,Critical Commercial

(4) Sirtuin 1 Activation Mitigates Murine Vasculitis Severity by Promoting Autophagy and MitophagybioRxivSeptember 11, 2025Asli E. Atici, Prasant K. Jena, Thacyana T. Carvalho, Benjamin L. Ross, Emily A. Aubuchon, Malcolm E. Lane, Angela C. Gomez, Youngho Lee, Shuang Chen, Timothy R. Crother, et al.LCWE (60 μg/ml) or LPS (10ng/ml; Invivogen, tlrl-3pelps) together with IFNγ (50ng/ml; R&D Systems, 485-MI-100/CF) for M1 polarization or IL-4 (10ng/ml; Tonbo Biosciences, 21–8041-U020) for M2 polarization for 24 hours.

(5) Dissecting FAP+ Cell Diversity in Pancreatic Cancer Uncovers an Interferon-Response Subtype of Cancer-Associated Fibroblasts with Tumor-Restraining PropertiesCancer researchJuly 2, 2025Cumming J, Maneshi P, Dongre M, Alsaed T, Dehghan-Nayeri MJ, Ling A, Pietras K, Patthey C, Öhlund D.established in splitting media or splitting media with recombinant murine IFNγ 10 ηg/mL ( R&D Systems, 485-MI-100/CF). After 4 days, media were refreshed with splitting media or splitting media with IFNγ. For STING agonist

(6) Intracellular methylglyoxal accumulation in classically activated mouse macrophages is mediated by HIF-1αJournal of Leukocyte BiologyMarch 14, 2025Daniel Prantner, Stefanie N. Vogelantibodies Escherichia coli K235 LPS was prepared as previously described.43 Recombinant mouse IFN-γ (# 485-MI-100/CF) and recombinant human IFN-γ (# 285-IF-100) were purchased from R & D systems (Minneapolis, MN, USA).

(7) Contribution of brain pericytes to neuroinflammation following repetitive head traumaJ Inflamm (Lond)March 3, 2025Arianna Cembran, Maxwell Eisenbaum, Daniel Paris, Michael Mullan, Fiona Crawford, Scott Ferguson, Corbin Bachmeier24",R&D systems,410-MT-025/CF IL1β,100,"2, 24",R&D systems,201-LB-010/CF IFN-γ,100,"2, 24",R&D systems,485-MI-100/CF Growth factor,Conc. (ng/mL),Incubation (h),Supplier,Cat No. PDGF-BB,10,24,Abcam,Ab78593 Specifications

(8) Mechanosensitivity of macrophage polarization: comparing small molecule leukadherin-1 to substrate stiffnessFrontiers in immunologyJanuary 1, 2025Joshi H, Anaya E, Addanki A, Almgren-Bell A, Todd EM, Morley SC.polarization BMDMs were cultured for 24 h with LPS (100 ng/ml) plus IFNγ (50 ng/ml) (R&D systems Cat.# 485-MI-100/CF) for classical activation (host defense) and with IL-4 (Abcam cat.# ab259406) plus IL-13 (BD biosciences

(9) Inflammation associated with monocyte/macrophage activation and recruitment corresponds with lethal outcome in a mouse model of Crimean-Congo haemorrhagic fever1Emerging Microbes & InfectionsDecember 31, 2024Teresa E. Sorvillo, Jana M. Ritter, Stephen R. Welch, JoAnn D. Coleman-McCray, Katherine A. Davies, Heather M. Hayes, Scott D. Pegan, Joel M. Montgomery, Éric Bergeron, Christina F. Spiropoulou, et al.by incubating with 250 ng/mL lipopolysaccharide and 20 ng/mL mouse or human IFN-gamma (R&D Systems, 485-MI-100/CF or 285-IF-100/CF) for 48 h [19,20]. Statistical analyses All statistical analyses were conducted using

(10) Long-lived lung megakaryocytes contribute to platelet recovery in thrombocytopenia modelsJournal of Clinical InvestigationNovember 15, 2024Alison C. Livada, Kathleen E. McGrath, Michael W. Malloy, Chen Li, Sara K. Ture, Paul D. Kingsley, Anne D. Koniski, Leah A. Vit, Katherine E. Nolan, Deanne Mickelsen, et al.was given at 1 mg/kg as a 1-time dose (MilliporeSigma, L6259). Recombinant mouse IFN-γ (R&D Systems, 485MI100/CF) was administered once daily i.p. at 0.04 mg/kg for a total of 3 consecutive days. Plasmodium yoelli

(11) Inflammation-induced epigenetic imprinting regulates intestinal stem cellsCell Stem CellOctober 3, 2024Dongchang Zhao, Visweswaran Ravikumar, Tyler J. Leach, Daniel Kraushaar, Emma Lauder, Lu Li, Yaping Sun, Katherine Oravecz-Wilson, Evan T. Keller, Fengju Chen, et al.basement membrane,Corning,Cat# 356230 JAG-1 protein,AnaSpec,Cat# AS-61298 Recombinant mouse IFN-gamma protein,R&D systems,Cat# 485-MI-100/CF Critical commercial assays,Critical commercial assays,Critical commercial assays Succinate Assay Kit,Abcam,Cat#

(12) Machine Learning-Directed Conversion of Glioblastoma Cells to Dendritic Cell-Like Antigen-Presenting Cells as Cancer ImmunotherapyCancer immunology researchOctober 1, 2024Liu T, Jin D, Le SB, Chen D, Sebastian M, Riva A, Liu R, Tran DD.to parent KR158 GBM cells, we pretreated parent KR158 GBM cells with 100ng/ml IFNγ (R&D Systems, Cat#485-MI-100/CF) for 3 hrs to upregulate MHCI and MHCII expression and thoroughly washed off the excess IFNγ prior to

(13) Long-lived lung megakaryocytes contribute to platelet recovery in thrombocytopenia modelsJ Clin InvestSeptember 19, 2024Alison C. Livada, Kathleen E. McGrath, Michael W. Malloy, Chen Li, Sara K. Ture, Paul D. Kingsley, Anne D. Koniski, Leah A. Vit, Katherine E. Nolan, Deanne Mickelsen, et al.was given at 1 mg/kg as a 1-time dose (MilliporeSigma, L6259). Recombinant mouse IFN-γ (R&D Systems, 485MI100/CF) was administered once daily i.p. at 0.04 mg/kg for a total of 3 consecutive days. Plasmodium yoelli

(14) The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathologyNature immunologyMarch 1, 2024Linnerbauer M, Lößlein L, Vandrey O, Peter A, Han Y, Tsaktanis T, Wogram E, Needhamsen M, Kular L, Nagel L, et al.(Peprotech, no. 211-11B) 100 ng ml−1, mouse HB-EGF (Novus Biologicals, no. 35069) 50 ng ml−1, IFN-γ (R&D, no. 485-MI-100/CF) 20 ng ml−1, I3S (Sigma, no. I3875-250MG) 50 µg ml−1, CH-223191 (Sigma Aldrich, no. C8124) 50 µM, Cobalt(II)-chloride

(15) SELENOP modifies sporadic colorectal carcinogenesis and WNT signaling activity through LRP5/6 interactionsJ Clin InvestJuly 3, 2023Jennifer M. Pilat, Rachel E. Brown, Zhengyi Chen, Nathaniel J. Berle, Adrian P. Othon, M. Kay Washington, Shruti A. Anant, Suguru Kurokawa, Victoria H. Ng, Joshua J. Thompson, et al.supplemented with 10% (v/v) FBS, 1% (v/v) penicillin/streptomycin, and 10 U/mL recombinant mouse IFN-γ (485MI100/CF, R&D Systems), and cultured at 33°C in 5% CO2. All cells used for experiments were passaged fewer than

(16) Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrainBrain, behavior, and immunityJuly 1, 2023Hobson BD, Stanley AT, De Los Santos MB, Culbertson B, Mosharov EV, Sims PA, Sulzer D.,Perkin Elmer,NEL745001KT,NA,"FISH, 1:1500IHC, 1:7500" Recombinant Mouse IFN-gamma Protein,R&D Systems (Biotechne),485-MI-100/CF,NA,100 ng/μL FANS: fluorescence-activated nuclear sorting, IHC: immunohistochemical staining of brain

(17) Facile repurposing of peptide-MHC-restricted antibodies for cancer immunotherapyNature biotechnologyJuly 1, 2023Yang X, Nishimiya D, Löchte S, Jude KM, Borowska M, Savvides CS, Dougan M, Su L, Zhao X, Piehler J, et al.

(18) Reduced mitochondrial calcium uptake in macrophages is a major driver of inflammagingNat AgingJuly 1, 2023Seegren PV, Harper LR, Downs TK, Zhao XY, Viswanathan SB, Stremska ME, Olson RJ, Kennedy J, Ewald SE, Kumar P, et al.HY-16082; 1 µM). For M1 versus M2 polarization, BMDMs were treated either with IFN-γ (R&D Systems, 485-MI-100/CF; 100 ng ml−1) and LPS (Invivogen, tlrl-eblps; 100 ng ml−1; to induce polarization toward a M1 phenotype)

(19) ATG16L1 protects from interferon-γ-induced cell death in the small intestinal cryptMucosal immunologyApril 1, 2023Foerster EG, Tsang DKL, Goyal S, Robertson SJ, Robert LM, Maughan H, Streutker CJ, Girardin SE, Philpott DJ.

(20) Single molecule analyses reveal dynamics of Salmonella translocated effector proteins in host cell endomembranesNature communicationsMarch 4, 2023Göser V, Sander N, Schulte M, Scharte F, Franzkoch R, Liss V, Psathaki OE, Hensel M.

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12/20/2022Great Mouse IFN-Gamma Protein Lucy LiuI used this mouse IFN-gamma (IFN?) Protein in mice bone-marrow-derived macrophage cells (BMDM). BMDMs were induced to M1 macrophage cells by 10 ng mL-1 IFN? and 10 ng mL-1 LPS. It works great, and ... Read Review

Recombinant Mouse IFN-gamma Protein, CF

IFN-gamma Protein

IFN-gamma Protein

IFN-gamma Protein

Recombinant Mouse IFN-gamma Protein, CF from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

Citations (423)

Reviews

Recombinant Mouse IFN-gamma Protein, CF from R&D Systems, a Bio-Techne Brand

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