Great Reagent for PNH

December 16, 2022

BlueskyReddit
UMass
Pathology
Lab Technician

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Company:

Cedarlane

Product Name:

FLAER (Alexa 488 proaerolysin variant) liquid format

Catalog Number:

FL2S

In our lab, we test for paroxysmal nocturnal hemoglobinuria (PNH). Although there are antibodies for this application, the addition of FLAER has revolutionized the testing! It binds to all GPI-anchors, which are lost in PNH cells.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

White blood cells (human)

Tips

Make sure it is titrated carefully.

Results Summary

All intact WBCs should be positive for FLAER. The only negative cells are those with a PNH phenotype (or non-WBC, e.g., platelets).

DOI or PMID #

N/A

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Summary

The Good

Accurate and highly concentrated.

The Bad

Can only be sourced from this supplier!

The Bottom Line

This is a good bright reagent that has become central to the work we do in PNH research. It is titratable down to a very small volume per test.

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