Fig 1: Neurogenic hypertension and cardiac mitochondrial defective OXPHOS subunit assembly/import. (A) Left, Western blot showed mitochondrial OXPHOS complex protein level in control and NG-HTN whole hearts lysate (total OXPHOS WB antibody cocktail, cat# ab110413, Abcam). Right, (i–iii) WB quantification of mitochondrial Complex-I/III, Complex-IV/III, and Complex-V/III ratio, respectively. (B) Left, Western blots showed mitochondrial OXPHOS complex protein level in cytosol vs. mitochondria fraction in control and NG-HTN hearts. Right, (i–iii) Western blot quantification of mitochondrial Complex-I/III, Complex-IV/III, and Complex-V/III ratio, respectively. Values are mean ± SEM, dot represents n = 6 rats, *P < 0.05 vs. Control, (A), Student’s t-test, (B); two-way ANOVA with Tukey’s multiple comparison test.
Fig 2: Relative levels of mtDNA and a subset of oxidative phosphorylation (OXPHOS) subunits in rat heart mitochondria from four experimental groups. (A). Relative amount of the mtDNA copy number in the heart tissue of four animal groups. The relative copy number of each group was calculated as the ratio between the mtDNA and nuclear DNA copy numbers. Data are expressed as mean values ± standard error from six independent experiments. (B). Representative Western blot of typical subunits of complexes I–V of the respiratory chain (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1, and CV-ATP5A) in the mitochondria (10 μg of protein per lane) isolated from the hearts of experimental rats. The analysis was carried out using the Total OXPHOS Rodent WB Antibody Cocktail (Abcam, #ab110413, Cambridge, UK) and polyclonal anti-VDAC1 antibodies (Abcam, #ab15895, Cambridge, UK). (C). Densitometric analysis of the content of the OXPHOS subunits in the heart mitochondria of rats from four experimental groups. Data are expressed as mean values ± standard deviations of three independent experiments. * p < 0.05 compared to the control group (CTR). Control values are taken as 100%.
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