Easy to Use CD38 Antibody

Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Beckman Coulter

Product Name:

CD38-APC-A750, LS198-4-3, 0.5 mL, ASR

Catalog Number:

A86049

Clone Number:

LS198-4-3

Info:Supplier Page 

Browse Similar Products: CD38 Antibodies

CD38 is an antigen that is typically classified as a 'secondary' marker. It does not help in lineage identification as it is expressed on a variety of cells, including activated T and B cells, NK cells, and monocytes (and brightly on plasma cells). In our lab's work, we also use it as a prognostic marker for CLL. Here, I show that this antibody stains the cells it is expected to, but is it bright enough?

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood

Primary Incubation

No dilution; 5uL per test

Blocking Agent

Bovine serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Red laser (FL-8 on Navios)

Results Summary

The results show a normal peripheral blood specimen stained with this antibody, I highlighted the monocytes (in red) to show that there was a good resolution on these cells. The lymphocytes have variable expression (depending on their activation status), while the granulocytes are negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and optimize.

The Bad

Lots of spillover issues.

The Bottom Line

A good antibody that is bright for most applications, but worth testing to check it is good enough for your specific assay.

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