DAPI from BioLegend

Product Specs
ItemDAPI
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number422801
Quantity10MG
ApplicationsICFC,ICC
FormatLyophilized
TargetDAPI

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Ilexoside K Ameliorates Atherosclerosis by Suppressing CD72hi Macrophages and Blocking CXCL12-CXCR4 Axis to Inhibit Endothelial PyroptosisAdv Sci (Weinh)September 27, 2026Xingling He, Sijing Li, Jiahui Chen, Liyu Lin, Xinyu Li, Xiaojiao Zhang, Yihui Zhang, Xingling Chen, Jinjian Guo, Zhongqi Yang, et al.staining buffer and resuspended in cold sorting buffer. Immediately prior to sorting, DAPI (Cat. No. 422801, BioLegend) was added to identify and exclude dead cells. Fluorescence‐minus‐one (FMO) controls were

(2) HIGH DOSE GAMMAHERPESVIRUS INFECTION IN MACROPHAGES RESULTS IN INFLAMMATORY MULTIMODAL CELL DEATHbioRxivSeptember 19, 2026Gabrielle Vragel, Gracyn Nelson-Reid, Rachael E. Kostelecky, Elizabeth A. Spear, Moses Lee, Derek W. Abbott, Linda F. van Dyk, Eric T. ClambeyJ6899.AK) and stained with DAPI (200 nM; 4',6-Diamidino-2-Phenylindole, Dilactate, Biolegend, Cat No. 422801) following manufacturer’s protocol, visualized using a 20X phase objective on EVOS FL. Flow Cytometry J774,

(3) Extracellular matrix composition and stiffness regulates progesterone responsiveness and decidualizationiScienceSeptember 18, 2026Danielle Klunk, Laura Homewood, Daniel AbebayehuScientific, A-21235) for 1 h at room temperature. Samples were then incubated with 500 ng/mL DAPI (BioLegend, 422801) for 15 min, then imaged with Leica Mica microscope within 2 h of staining. All quantification was performed

(4) Tumor-infiltrating plasma cell profiling after PD-1 blockade reveals tumor-specific antibodiesCancer CellSeptember 14, 2026R. Ryan Meyerhoff, Alan Chen, Jenny O’Brien, Carmen M. Anadon, Luis Uriel Lopez Lopez Bailon, Dayan Carrion-Estrada, Koravit Poysungnoen, Jared Lindenberger, Gabor Kemeny, Liliana Lyniv, et al.Cocktail,Sigma,#11697498001 IL-2,PeproTech,Cat# 200-02 Recombinant PAD2,Cayman Chemical,#10785 DAPI,Biolegend,# 422801 Zombie Yellow Fixable Viability Kit,Biolegend,# 423103 Ficoll-Paque gradient,GE Healthcare Systems,#17-1449-02 Critical

(5) Simultaneous gene engineering of T cells with multiple neoantigen-specific T cell receptors for adoptive cell transferMol Ther AdvSeptember 10, 2026Melinda Magna, Lior Levy, Krisztian Csomos, Aaron J. Dinerman, Abraham A. Hakim, Noam Levin, Sanghyun P. Kim, Nikolaos Zacharakis, Neilesh B. Parikh, Brian D. Bui, et al.anti-mouse TCR-β FITC (BD #553171) propidium iodide (#P1304MP, Thermo Fisher), or DAPI (BioLegend, #422801). Phenotyping panel: live/dead stain (eBioscience fixable viability dye eFluor 780, #65-0865-14), CD3-BUV395

(6) In vivo selection of anti-glioblastoma DNA aptamer-drug conjugates in an orthotopic patient-derived xenograft modelNAR CancerSeptember 3, 2026Caroline D Doherty, Sonia Jain, Lauren L Ott, Katie K Bakken, Brandon A Wilbanks, Danielle M Burgenske, Keenan S Pearson, Jessica I Griffith, Zichen Tian, Jeffrey A Meridew, et al.were washed four times with PBS, and slides were then stained with 300 nM DAPI solution (Biolegend #422801) prepared in PBS for 5 m. After washing twice with PBS, slides were mounted using mounting media (ProLong

(7) Comprehensive Analyses of SOX7 Provide Novel Insights on Its Tumor Suppressor Role and Its Target Genes with Therapeutic Implications in Multiple MyelomaInternational Journal of Molecular SciencesAugust 26, 2026Arda Ceylan, Xiaozhou Hu, Tevfik Hatipoğlu, Fenangi Chiara Nainkwi, Kadriye Bahriye Payzın, Güner Hayri Özsan, Itır Şirinoğlu Demiriz, Ömer Şeker, Merve Kakçı, Osman Can Öztürk, et al.Cycle Analyses Cell cycle distribution was analyzed with DAPI (BioLegend, San Diego, CA, USA, Cat no: 422801) staining 4 days post-transduction. Briefly, cells were fixed with the True-Nuclear Transcription Factor

(8) Rapid discovery of monoclonal antibodies via high-throughput single BCR affinity sequencingNucleic Acids ResAugust 20, 2026Mengzhu Hu, Qiuyu Lian, Xiaonan Cui, Xiao Chen, Xue Dong, Mengge Huang, Guangchao Wang, Yan Hu, Hao Zhang, Jiacan Su, et al.were washed twice after 30 min of incubation on ice. DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate, 422801, Biolegend) was used to label dead cells. DAPI−/B220 PE+/antigen AF647+ cells were sorted with a CytoFLEX

(9) Propionic Acid Remodels Mitochondrial Metabolism in SH-SY5Y CellsBiology (Basel)August 18, 2026Caitlyn Mahony, Erin Buchanan, Colleen O'Ryan90 min at 4 °C. Lastly, samples were counterstained with 250 nM Dapi (BioLegend, San Diego, CA, USA, 422801) for 10 min, washed and mounted on 26 × 76 mm × 1.2 mm glass microscope slides using 7 μL of Antifade

(10) P4HA3-mediated hydroxylation of SLC7A11 drives tyrosine kinase inhibitor resistance by suppressing ferroptosis in clear cell renal cell carcinomaRedox BiolAugust 14, 2026Yueming Chen, Guixin Zhang, Ruixin Yang, Yifan Li, Haozhuo Song, Zeping Chen, Hao Cai, Qingquan Li, Chen Fang, Jingyi Huang, et al.antibodies for 1 h at room temperature in the dark. Nuclei were counterstained with DAPI (Biolegend, #422801). Fluorescence images were acquired using a confocal laser scanning microscope. 2.18 Gene expression

(11) Genome-wide CRISPR screening identifies cellular factors controlling nonviral genome editing efficiencyNat CommunAugust 13, 2026Shivani Saxena, Meha Kabra, Amr A. Abdeen, Diana M. Tabima, Divya Sinha, Piper A. Rawding, Min Zhu, Ruosen Xie, Tanvi Kulkarni, Giovanni M. Hanstad, et al.488–conjugated donkey anti-mouse (Invitrogen, A21202, 1:500) secondary antibodies were used. DAPI (Biolegend, 422801, 1:1000) labeled the nucleus. A confocal microscope (Nikon C2 Instruments) was used to capture z-stack

(12) Humanizing acidic mammalian chitinase variants establish lung immune conditioning and control environmentally driven inflammation and fibrosisCell ReportsAugust 12, 2026Yilin Wang, Haerin Jung, Do-Hyun Kim, Eilene Liu, Donovan Watza, Samuel I. Risma, Kira L. Florczak, Leah M. Kim, Zeena Bou Bou Reslan, Ananya Gupta, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies DAPI,BioLegend,Cat#422801 Anti-mouse CD45 antibody (clone 30-F11),BD Biosciences,Cat#564279; RRID: AB_2651134 Anti-mouse CD3

(13) Lysosomal dysfunction drives a transcriptional and epigenetic signature characteristic of disease-associated microglia in neurodegenerative diseasesImmunityAugust 11, 2026Christopher D. Balak, Johannes C.M. Schlachetzki, Addison J. Lana, Elizabeth West, Christine Hong, Jordan DuGal, Yi Zhou, Benjamin Li, Nathanael J. Spann, Payam Saisan, et al.centrifuged at 800xg for 5 minutes at 4°C in a swinging bucket centrifuge. DAPI (1:1,000; BioLegend 422801) was used to label nuclei. Samples were strained through a 70 μm mesh strainer, washed once more, resuspended

(14) Temporal changes in metabolism guide oligodendrocyte precursor cell dynamics in aging and multiple sclerosisNeuronAugust 7, 2026Tess Dierckx, Sarah E. Wilson, Rebecca Buchanan, Themistoklis M. Tsarouchas, Yuhsiang Cheng, Maria Sacconi Nunez, Kelly Strickland, Dena Kumsa, Yohan S. S. Auguste, Sam Vanherle, et al.Afterwards, the cells were detached with 0.05% Trypsin-EDTA (Thermofisher, 25300054), DAPI (Biolegend, 422801) was added to determine cell viability, and MitoSox MFI was assessed in the DAPI-MitoSox+ cell population

(15) Cadherin 1 negatively regulates SARS-CoV-2 infection in the olfactory epitheliumEMBO ReportsAugust 3, 2026Weihao Li, Yuansen Shi, Xuewen Li, Meiqin Liu, Jinxia Liu, Zhen Qin, Haofeng Lin, Yuzhen Wang, Yiqun YuAPC anti-mouse/human CD324 (E-Cadherin),BioLegend,Cat #147311 APC/Cyanine7 anti-His-Tag,BioLegend,Cat #362633 DAPI,BioLegend,Cat #422801 Oligonucleotides and other sequence-based reagents,Oligonucleotides and other sequence-based reagents,Oligonucleotides

(16) Protocol for generation of a 3D humanized lung-tumor-on-a-chip model of immune-hot and immune-cold microenvironmentsSTAR ProtocJuly 23, 2026Sabrina Brito Añez, Sanne van Kesteren, Peter Carmeliet, Anna Rita Cantelmo, Patricia Yagüe, Jeffrey Kroon,Cell Signaling Technology,8953 Mouse anti-human CD31 Alexa Fluor 647 1:200,BD Pharmingen,561654 DAPI 1:5000,BioLegend,422801 Hoechst 1:500,Thermo Fischer Scientific,62249 ,, Biological samples,Biological samples,Biological

(17) Emergency myelopoiesis in murine arthritis results in cell-intrinsic changes in dendritic cell development and functioniScienceJuly 17, 2026Lin Tze Tung, Roselyn R. Jiang, Bianca Pozzebon, Mathieu Mancini, Lucie Droin, Mitra Yousefi, Joo Eun (June) Kim, Marwah Mousa, Yue Liang, Connie M. Krawczyk, et al.Scientific,SB-4401-75 Viability Dye eFluor 506,ThermoFisher Scientific,65-0866-14 DAPI,Biolegend,422801 Fc blocker TruStain FcX,Biolegend,101320 Proliferation Dye eFluor 450,ThermoFisher Scientific,65-0842-85 Lipopolysaccharide,Millipore-Sigma,"LPS25,

(18) miR-205-5p promotes endothelial dysfunction and senescence in pulmonary fibrosisJCI InsightJuly 8, 2026Giuseppe Muscato, Benjamin B. Roos, Sharonda Harris, Xiaoyu Tracy Cai, Gina Civettini, Enrico Sciacca, Ahmed A. Raslan, Alessandra Castaldi, Sharon Elliot, Marilyn K. Glassberg, et al.(Thermo Fisher Scientific, 1:500 dilution, RRID:AB_2534069) and counterstained with DAPI (Biolegend, 422801, 1:1,000 dilution), which was added for 2 hours at room temperature. All antibodies were commercially

(19) Eliminating PD‐L1 on Dendritic Cell Extracellular Vesicles for Immunotherapy Potentiates Immune‐Mediated Tumour Rejection in MiceJournal of Extracellular VesiclesJuly 1, 2026Loes Teeuwen, Loïc Steiner, Chantal Reinhardt, Annemarijn Offens, Jesse E. Kuipers, Daniel Martínez‐Martínez, Jules Mazouin, Benedict J. Chambers, Gözde Güçlüler Akpinar, Susanne Gabrielssonco‐cultures were resuspended in 200 µL AnnexinV binding buffer or PBS, respectively, containing 3 µM DAPI (422801, BioLegend). Isolated cells from spleens, tumours and TDLNs were stained and incubated for 30 min at

(20) TROP2 targeting reveals therapy-driven cell state dynamics in colorectal cancerNatureJuly 1, 2026Nuria Vaquero-Siguero, Nikolaos Georgakopoulos, Maria C. Puschhof, Ioannis Chiotakakos, Jasmin Meier, Sigrid K. Fey, Gabriele Diamante, Manuel Mastel, Aitana Guiseris-Martinez, Guillaume Belthier, et al.FACS buffer (PBS with 1% fetal calf serum) and resuspended in 200-µl FACS buffer with DAPI (BioLegend; 422801) or Zombie NIR (BioLegend; 423105), which were used as death exclusion markers. The samples were analysed

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(1)

3/28/2023Great DAPI for Immunocytochemistry Umut TankI use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really low. So, it will last forever.
(1)

7/12/2022DAPI Nuclear Stain Matthew RatsepI use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.

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DAPI from BioLegend

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