Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb 9145S from Cell Signaling Technology

Product Specs
ItemPhospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $431.00 Inquire
Catalog Number9145S
Quantity100 µl
ApplicationsW, W-F, W-S, IP, IHC-Bond, IHC-P, IF-IC, FC-FP, ChIP, ChIP-seq
ReactivityH, M, R, Mk
TargetSTAT3 (Tyr705) phosphate
ModificationsSTAT3 (Tyr705) phosphate
HostRabbit
NCBI Gene AliasesADMIO, ADMIO1, APRF, HIES
ClonalityMonoclonal
NCBI Full Gene Namesignal transducer and activator of transcription 3
Research AreaImmunology and Inflammation
IsotypeIgG
CloneD3A7
Molecular Weight79, 86

Questions and Answers

What applications has the Phospho-Stat3 (Tyr705) antibody been shown to be effective in?

The Phospho-Stat3 (Tyr705) antibody has been utilized in various applications, including immunohistochemistry and Western blotting, to study the activation of the JAK2/STAT3 signaling pathway in different biological contexts, such as diabetic wound healing and cancer progression.

Can this antibody be used to investigate the role of Stat3 in cancer models?

Yes, the Phospho-Stat3 (Tyr705) antibody has been referenced in studies examining its role in cancer, such as glioma development, where it was shown that activation of the JAK/STAT signaling pathway is crucial for tumor progression, indicating its potential utility in cancer research.

Is there any evidence supporting the use of this antibody in studies related to wound healing?

Yes, the antibody has been cited in research demonstrating that exosomes derived from platelet-rich plasma can promote diabetic wound healing through the JAK2/STAT3 pathway, highlighting its relevance in studies focused on tissue regeneration and repair.

How does the Phospho-Stat3 (Tyr705) antibody contribute to understanding the mechanisms of apoptosis in cancer cells?

The antibody has been used in studies that explore the relationship between Stat3 activation and apoptosis regulation in cancer cells, such as in the context of targeting Bcl-xL in extranodal NK/T cell lymphoma, where the IL2-JAK1/3-STAT5 signaling pathway was implicated in Bcl-xL dysregulation, showcasing its role in apoptosis mechanisms.

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Website: Technical Support

(1) Ceapin-A7 counteracts the protective effects of Lanreotide in endothelial cellsAspects of Molecular MedicineJune 1, 2026Md Matiur Rahman Sarker, Saikat Fakir, Madan Sigdel, Nektarios BarabutispP38 (9211S), P38 (9212S), pSTAT1 (9167S), STAT1 (9172S), pERK1/2 (9101S), ERK1/2 (9102S), pSTAT3 (9145S), STAT3 (4904S), BiP (3183S), and Grp94 (2104S) antibodies were acquired from Cell Signaling Technology

(2) Ruxolitinib reverses alopecia areata via a triple mechanism: JAK-STAT inhibition, localized oxidative stress attenuation and selective apoptosis modulationJournal of Translational AutoimmunityJune 1, 2026Meiling Zhu, Hao Mu, Bo Wang, Mengting Han, Zhao Ma, Xiaopan Nie, Tong Wang, Liyuan Zhang, Feng Lianganti-p-STAT1 (1: 250; Bioss Antibodies, 52209R), and anti-p-Stat3 (1:50 dilution; Cell Signaling Technology, 9145s). After washing, sections were incubated with a biotinylated goat anti-rabbit IgG secondary antibody

(3) Kaempferol attenuates LPS-induced inflammatory responses in H9c2 cells through involvement of the IL-6/JAK2/STAT3 pathwayMol Biol RepApril 27, 2026Hongyi Yue, Yunfei Jia, Ruohao Sun, Hehe Liao, Yi Zuo, Pingyi Wang, Wenhua LiConcentration Measurement Kit (Cat# AR0146) were purchased from BOSTER. Phospho-STAT3 antibody (Cat# 9145) was purchased from Cell Signaling Technology (Shanghai) Biological Reagents Co., Ltd. Phospho-JAK2

(4) Targeting pancreatic cancer with combined inhibition of EGFR and RAFPLoS OneApril 24, 2026Jakob Brandstetter, Lea Goldstein, Tim Schreiber, Rupert Palme, Tobias Lindner, Markus Joksch, Bernd Krause, Brigitte Vollmar, Simone KumstelHRP-conjugated (polyclonal goat anti-mouse, 1:100 Dako, Germany Hamburg) or anti-phospho Stat3 (Tyr705) (#9145 Cell Signaling Technology, Danvers; Massachusetts, USA) followed by secondary antibody (goat anti rabbit

(5) HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasionCell Reports MedicineApril 21, 2026Bo Zeng, Yingying Zhou, Heping Wang, Panyin Shu, Ting Pan, Fangfang Liu, Yangyang Li, Ming Yi, Ruirui He, Lingyun Feng, et al.,Cell Signaling Technology,Cat# 9167; RRID: AB_561284 Phospho-Stat3 (Tyr705) Rabbit mAb,Cell Signaling Technology,Cat# 9145; RRID: AB_2491009 Mouse PD-L1 Rabbit mAb,Cell Signaling Technology,Cat# 60475; RRID: AB_2924680 Human

(6) Out-of-frame CBX3::ALK fusion drives ALK activation and therapy responseCell Reports MedicineApril 21, 2026Jen-Fan Hang, Han-Ying Cheng, Yu-Shuen Tsai, Sin-Ying Lin, Jie-Hong Song, Chih-Hung Chung, Muh-Hwa Yang,Cell Signaling Technology,Cat # 9139; RRID: AB_331757 Rabbit polyclonal anti-P-STAT3 (Tyr705),Cell Signaling Technology,Cat # 9145; RRID: AB_2491009 Rabbit polyclonal anti-Erk1/2,Cell Signaling Technology,Cat # 9102; RRID: AB_330744 Rabbit

(7) Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic woundsCell Reports MedicineApril 21, 2026Li Lu, Yuan Xiong, Jiewen Liao, Juan Zhou, Guangji Wang, Yating Qin, Shengming Zhang, Yanzhi Zhao, Xiaodan Zhong, Mengwen Wang, et al.,Cell Signaling Technology,Cat#9167; RRID: AB_561284 Rabbit monoclonal anti-p-STAT3,Cell Signaling Technology,Cat#9145; RRID: AB_2491009 Rabbit monoclonal anti-STING,Cell Signaling Technology,Cat#13647; RRID: AB_2732796 Rabbit

(8) Octreotide Counteracts IFN-γ-Induced Endothelial InflammationJ Biochem Mol ToxicolApril 19, 2026Saikat Fakir, Madan Sigdel, Md Matiur Rahman Sarker, Nektarios Barabutis(8505S), pP38 (9211S), P38 (9212S), pJAK2 (3776S), JAK2 (3230S), pSTAT1 (9167S), STAT1 (9172S), pSTAT3 (9145S), and STAT3 (4904S) antibodies were available from Cell Signaling Technology (Danvers, MA). 2.2 Cell Cultures

(9) KAT8 drives M2 macrophage polarization to exacerbate allergic airway inflammationiScienceApril 17, 2026Xianwen Lai, Han Li, Zhao Zhao, Yu Zhong, Guomei Su, Jiewen Huang, Yuanyuan Xiang, Ruina Huang, Jingyun Quan, Zhihang Feng, et al.AB_2839421 Anti-STAT3,Santa Cruz,Cat#sc8019; RRID: AB_628293 Anti-p-STAT3,Cell Signaling Technology,Cat#9145; RRID: AB_2491009 Anti-ARG1,Affinity Biosciences,Cat#DF6657; RRID: AB_2838619 ,, Biological samples,Biological

(10) An Optimized Conversion of Spermatogonial Stem Cells Into Spinal Cord Neurons Enhances Functional Recovery in Rats After Spinal Cord InjuryCNS Neurosci TherApril 15, 2026Xinyu Guo, Yongjie Zhang, Haihong Zhang, Hao Yang(3230), anti‐phospho‐Jak2 antibody (3771), anti‐Stat3 antibody (9139), and anti‐phospho‐Stat3 antibody (9145) were obtained from Cell Signaling Technology. 2.3 Isolation and Culture of Spermatogonial Stem Cells Primary

(11) Nerves Stimulates Crosstalk between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance ImmunosuppressionCancer ResearchApril 15, 2026Fangli Liao, Yanran Tong, Hua Sun, Sen Chen, Siyang Wen, Yan-e Du, Linshan Jiang, Tong Huang, Manran Liu, Weixian Chen, et al.#ET1607-38, RRID: AB_3069762, 1:2,000), anti–phospho-STAT3 (pSTAT3; Tyr705; Cell Signaling Technology, cat. #9145, RRID: AB_2491009, 1:2,000), anti-GPR34 (Abways, AY2411, 1:1,000), anti–HIF-1 alpha (Abcam, cat. #ab2185,

(12) Mechanistic study of MUC3A in promoting progression of clear cell renal cell carcinoma via the JAK‑STAT pathwayOncol RepApril 14, 2026Yongyang Yun, Xing Ji, Shuai Hu, Tianyu Wu, Yixiao Liu, Zheng Li, Zhoujie Sun, Peimin Zhou, Lei Yang, Wei Yu(rabbit monoclonal; cat. no. 9139; dilution, 1:1,000), p-STAT3 (Tyr705) (rabbit monoclonal; cat. no. #9145; dilution, 1:1,000), Bcl-2 (rabbit monoclonal; cat. no. #4223; dilution, 1:1,000), and cleaved Caspase-3

(13) Chemotherapy Enrichment of ID Family Expression Is Associated with IL-6 Signaling in Ovarian CancerCancers (Basel)April 8, 2026Megan Anne Keene, Darren Lighter, Cassandra Brenner, Ixchel Urbano, Katelyn Shelby, Samuel F. Gilbert, Mikella Robinson, Carrie D. HouseSanta Cruz Biotechnology, Dallas, TX, USA, 1:200). For tumor lysates, primary antibodies include P-STAT3 (9145, Cell Signaling Technology, Danvers, MA, USA, 1:1000), T-STAT3 (4904, Cell Signaling Technology, Danvers,

(14) Inhibition of CDK9 sensitizes multidrug resistant ovarian cancer cells to paclitaxelSci RepApril 7, 2026Jinglu Wang, Francis J. Hornicek, Huirong Shi, Wenlong Feng, Zhenfeng Duan(Cell Signaling Technology, Cat.#9139, 1:1000 dilution); Phospho-Stat3 (Cell Signaling Technology, Cat.#9145, 1:1000 dilution); Mcl-1 (Cell Signaling Technology, Cat.#39224, 1:1000 dilution); Bax (Cell Signaling Technology

(15) CD38 overexpression drives glioblastoma progression via L1CAM/ICAM1/JAK-STAT-Driven tumor microenvironment rewiringTransl OncolApril 6, 2026Saeed Norouzi, Sonikpreet Aulakhspecified): Phospho-Stat1 (Tyr701) (#58D6), Stat1 (#14,994), Stat3 (#12,640), Phospho-Stat3 (Tyr705) (#9145), Human CD38 (#51,000), Mouse CD38 (#68,336), p21 Waf1/Cip1 (#2947), p27 Kip1 (#3688), p16 INK4A (#29,971),

(16) Tissue-specific inflammation induces cell state plasticity with oncogenic addiction in mucosal melanomaSci AdvApril 3, 2026Xuhui Ma, Yanni Ma, Li Zhang, Ruixin Liu, Ronghui Xia, Meiling Hao, Xiaole Song, Yinan Chen, Yang Zheng, Hao Wang, et al.,Cell Signaling,2243 Anti-HER2/ErbB2,Cell Signaling,2165 Anti-HER3/ErbB3,Cell Signaling,12708 Anti-Phospho-STAT3 (Tyr705),Cell Signaling,9145 Anti-GP100,HUABIO,HA721719 Anti-Phospho-NF-κB p65 (S529),HUABIO,ET1604-27 Anti-NF-κB p65,HUABIO,ER0815 Anti-STAT3,HUABIO,ET1607-38 Anti-Beta

(17) High-fat diet and obesity each increase tumor cell proliferation and muscle wasting in experimental cancer cachexiaAmerican Journal of Physiology-Cell PhysiologyApril 1, 2026Brittany R. Counts, Andrea Bonetto, Ho Lam Chan, Ernie D. Au, Yanlin Jiang, Marion E. Couch, Denis C. Guttridge, Michael C. Ostrowski, Leonidas G. Koniaris, Teresa A. ZimmersDura, Thermo Fisher Scientific, Suwanee, GA) and exposure to film. Antibodies used were: pSTAT3-Y705 (9145), STAT3 (9132), p-AKT-S473 (4060), AKT (4685), p-Foxo3a-S381/321 (9465), Foxo3a (2497), and GAPDH (2118)

(18) Kupffer Cell-Derived Interleukin-6 Aggravates Radiation-Induced Liver Disease by Activating Hepatocyte STAT3 to Promote Ccng1 TranscriptionAdvances in Radiation OncologyApril 1, 2026Xing Song, Yingjie Shao, Rui Sun, Wenjie Jiang, Yuan Chen, Mengjiao Liu, Wendong Gu, Xiao Zheng, Jingting Jiangsonication, immunoprecipitation was performed with a p-STAT3 (Tyr705) antibody (Cell Signaling Technology, #9145). The immunoprecipitated complex was subsequently washed. The DNA was then extracted and purified with

(19) A uniquely leptin sensitive hypothalamic neuron population limits hyperphagia and weight gain in diet-induced obesitybioRxivMarch 30, 2026Dylan M Belmont-Rausch, Benedicte Schultz Kapel, Abigail J. Tomlinson, Chao Ding, Allison M. Duensing, Bernd Coester, Cecilie Vad Mathiesen, Leonie Cabot, Jenny M. Brown, Charlotte Høy Kruse, et al.performed in KPBS with 1% NGS and 0.3% Triton X-100 with rabbit anti-pSTAT3 (Cell Signaling Technology, #9145; 1:200) overnight at 4°C. Slides were washed five times for 5 minutes each with 0.02 M KPBS. They were

(20) Tumor growth inhibitory activity of the P2X7 receptor antagonist AZ10606120 in two cell lines of human glioblastomaPurinergic SignalMarch 28, 2026Michela Graziano, Silvia Bono, Michela Pizzoferrato, Chiara Ferraro, Flavia Grossi, Pierluigi Navarra, Maria Martire, Lucia Lisi,Novus Biologicals,NB500-249 Cleaved caspase 3 (Asp 175),1:1000,Cell signaling,#9661 pSTAT 3,1:500,Cell signaling,#9145 totSTAT 3,1:1000,Cell signaling, NFKB,1:1000,Cell signaling,#8242 GAPDH,1:1000,Cell signaling,#2118 Anti-mouse,1:3000,Sigma-Aldrich,#A

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(1)

10/21/2022Good Phospho-STAT3 Antibody YONG MTo determine whether IL-6 gene transfer affects phosphorylated STAT3 expression. The results showed IL-6 enhanced STAT3 protein phosphorylation in obese mice.
(1)

6/15/2022Good Phospho-STAT3 Antibody for Protein Quantification Vanessa KelleyWe knocked out an upstream JAK protein in the Cal27 cell line and wanted to examine downstream STAT3 signaling. We blotted JAK KO (KO) and control cell lines for Phospho-Y705 STAT3 in starvation and ... Read Review
(1)

9/17/2020Phospho-STAT3 Activation in the ARC Deng GuoLeptin activates STAT3 though its receptor in the hypothalamus of the brain, which regulates body weight and energy expenditure.
(1)

2/28/2018Detection Of phospho-STAT3 By Western Blot Ab GThe aim of my research is to evaluate effects of novel drugs on Wnt signaling in breast cancer. I wanted to detect whether the drugs affected the STAT3 pathway, and pSTAT3 is an active mediator. The ... Read Review
(1)

9/14/2017Detection Of Phospho-STAT3 From Mouse CD4 T Cells Jeeho LeeThe purpose is to detect phospho-STAT3 from mouse CD4 T cells in response to IL-6.
(1)

3/7/2016Best Phospho-STAT3 Antibody A MOur lab works with kinases involved in kidney cancer, including STAT3. We needed to reliably detect low protein levels of phosphorylated STAT3 (at Tyr705) in 786-0 cell lysates, and had no problem ... Read Review
(1)

1/26/2016Good Phospho STAT3 Antibody Franz RicklefsI am investigating the role of the STAT3-Pathway between different cancerous cell lines.
(1)

7/8/2015Great phospho-STAT3 antibody J GI use this antibody to detect the levels of STAT3 phosphorylated at tyrosine 705 to see the affects different signaling pathway inhibitors have on STAT3 signaling. I like this antibody because it ... Read Review

Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb

Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb from Cell Signaling Technology

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