TFAM (D5C8) Rabbit mAb 8076S from Cell Signaling Technology

Product Specs
ItemTFAM (D5C8) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8076S
Quantity100 µl
ApplicationsW, W-S, IP, IHC-P, IF-IC, eCLIP
ReactivityH
TargetTFAM
HostRabbit
NCBI Gene AliasesMTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3
ClonalityMonoclonal
NCBI Full Gene Nametranscription factor A, mitochondrial
Research AreaNeuroscience
IsotypeIgG
CloneD5C8
Molecular Weight24

Questions and Answers

Can this antibody be used for Western blotting, and if so, what dilution is recommended?

Yes, the TFAM (D5C8) Rabbit mAb has been shown to work in Western blotting applications. A dilution of 1:500 is recommended for optimal results in this assay.

Is there any evidence of this antibody being used in immunofluorescence assays?

Yes, the TFAM (D5C8) Rabbit mAb has been utilized in immunofluorescence assays, with a recommended dilution of 1:100, as noted in various studies.

How does this antibody contribute to research on mitochondrial function?

The TFAM antibody is critical for studies investigating mitochondrial biogenesis and function, as it recognizes the mitochondrial transcription factor A (TFAM), which plays a key role in mitochondrial DNA maintenance and transcription. Research has demonstrated its involvement in mitochondrial metabolism and bioenergetics, particularly in the context of diseases like heart failure and cancer.

What species does this antibody react with, and is it suitable for human studies?

The TFAM (D5C8) Rabbit mAb is specifically reactive with human (Homo sapiens) TFAM protein, making it suitable for studies involving human samples.

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.(#2118), STING (#13647), pTBK1 S172 (5483), TBK1 (#3504), pIRF3 S396 (#4947), IRF3 (#4302), TFAM (#8076), HSP90 (#4877), pUB S65 (#62802), COX4 (#4850), pPINK1 S228 (#89010), STOML2 (#89199), Vinculin (#13901)

(2) MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmyNat Struct Mol BiolApril 1, 2026Stephen P. Burr, Kathryn Auckland, Angelos Glynos, Abhilesh Dhawanjewar, Cameron Ryall, Wei Wei, Antony Hynes-Allen, Malwina Prater, Matylda Sczaniecka-Clift, Julien Prudent, et al.incubation was performed at room temperature for 1 h. Primary antibodies were anti-TFAM (Cell Signaling, 8076S) and anti-vinculin (Sigma, V4505). The Clean-Blot immunoprecipitate detection kit (horseradish peroxidase)

(3) Bone morphogenetic protein receptor 2 signaling mediates mitochondrial Ca2+ transport through its regulation of TAK1 splice variantCell Communication and SignalingFebruary 11, 2026Ashley Toussaint, Manohar Singh, Hellen Chiou, Guoqiang Wang, Dongxuan Jia, Monica Driscoll, Vrushank Bhatt, Jean De La Croix Ndong, Sahil Shuaib, Harrison Zoltowski, et al.Smad1(Cat. No. 6944), TAB1 (Cat. No. 3226), BMPR2 (Cat. No. 24803), MCU (Cat. No. 14997), TFAM (Cat. No. 8076), MT-ATP8 (Cat. No. 17412), and phospho-TAK1 (Thr184/187) (Cat. No. 4508) were purchased from Cell Signaling Technology

(4) Cold Atmospheric Plasma Promotes Anti-Inflammatory and Regenerative Responses in Oral Soft Tissue through Redox-Driven Mitochondrial RegulationACS OmegaJanuary 20, 2026Tien Ngoc Thuy Nguyen, Byung-Hoon Kim(S36002). Primary antibodies against NF-κB p65 (8242S), phospho-NF-κB p65 (3033S), PGC-1α (2178S), TFAM (8076S), and HSP60 (12165) were from Cell Signaling Technology (Danvers, MA, USA), while β-actin (ab8224) and

(5) Mitochondrial HMGCS1 mediates cisplatin resistance in cervical cancer through regulation of mitochondrial transcriptionBMC Molecular and Cell BiologyJanuary 16, 2026Wenxuan Yang, Shumin Liu, Dandan Shang, Ping Liu, Hongtang Shi, Hongtao Zhang, Chao Zhouwith antibodies against HMGCS1 (Abcam, ab194971), POLRMT (Abcam, ab228576), TFAM (Cell Signaling, #8076), or TFB2M (Abcam, ab228614) overnight at 4 °C. Immune complexes were captured with Protein A/G agarose

(6) Alterations in Mitochondrial DNA in Corneal Fibroblast and Myofibroblast Post InjuryInvestigative Ophthalmology & Visual ScienceJanuary 16, 2026Nishant R. Sinha, Alexandria C. Hofmann, Laila A. Suleiman, Maxwell T. Jeffrey, Rajnish Kumar, Rajiv R. MohanThereafter, cells received three more washes with PBS and the primary TFAM antibody at 1:200 dilution (8076T; Cell Signaling Technology, Danvers, MA, USA) or αSMA at 1:500 (GA611; Agilent Technologies) overnight

(7) Nucleotide metabolic rewiring enables NLRP3 inflammasome hyperactivation in obesityScienceJanuary 15, 2026Danhui Liu, Chuanli Zhou, Xiaochen Wang, Zhou Luo, Ruiyao Xu, Shanshan Huo, Lina Guo, Xuemei Luo, Shuhan Yang, Arielle Click, et al.anti-CMPK2 (NBP1–80653, Novus Biologicals), anti-mouse TFAM (ARP36993, Avivabio), anti-human TFAM (8076S, Cell Signaling Technologies), anti- PP2A-B55 (4593S, Cell Signaling Technologies), anti-p21 Waf1/Cip1

(8) SNAP23 deficiency triggers Trim21 mitochondrial translocation to suppress TFAM-mediated oxidative metabolism and drive chemoresistance in colorectal cancerCell Death & DiseaseNovember 22, 2025Abudushalamu Yalikun, Bingjie Guan, Jiawei Pan, Haonan Chen, Jingfeng Cai, Ziyan Zhu, Runkai Zhou, Bowen Xie, Youdong Liu, Jikun Li(for WB, IP)",CST,"Cat# 92043,RRID:AB_2800177" "TFAM (D5C8) Rabbit mAb (for WB, IP, IHC)",CST,"Cat# 8076, RRID:AB_10949110" Ubiquitin (E6K4Y) XP® Rabbit mAb (for WB),CST,"Cat# 20326, RRID:AB_3064918" Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb

(9) Estrogen-Related Receptor Alpha Promotes Skeletal Muscle Regeneration and Mitigates Muscular DystrophyFASEB JOctober 15, 2025Thi Thu Hao Nguyen, Ya Xiang Huang, Svitlana Poliakova, Citu Citu, Eira Mann, Danesh H. Sopariwala, Zhongming Zhao, Ashok Kumar, Vihang A. NarkarThermo Fisher Scientific, 1:1000), total OXPHOS rodent antibody cocktail (ab110413, Abcam, 1:1000), TFAM (8076, Cell signaling, 1:1000), CYCS (sc13561, Santa Cruz Biotechnology, 1:500). The membranes were washed

(10) Zinc Promotes Mitochondrial Health Through PGC-1alpha Enhancing Bacterial Clearance in Macrophages Infected with Mycobacterium avium ComplexInternational Journal of Molecular SciencesSeptember 23, 2025Ruxana T. Sadikot, Prabagaran Narayanasamy, Zhihong Yuan, Deandra Smith, Daren L. KnoellDanvers, MA, USA, 2178), PGC-1α (Santa Cruz Biotechnology (SCBT), Dallas, TX, USA, sc-518025), TFAM (CST, 8076), mtTFA (SCBT, sc-166965), β-Actin (CST, 3700), β-Tubulin (CST, 2146) anti-rabbit IgG HRP (CST, 7074),

(11) miR‑34a induces apoptosis and pyroptosis in D‑Galactose‑induced aging cochlear hair cells via inhibiting TFAM and promoting mitochondrial dysfunction in vitro and in vivoInt J Mol MedApril 30, 2025Yilan Wang, Ming Yang, Guihua Wang, Weimin Liu, Bin Deng, Xiaoran Yang, Xuzhao LiInc.), anti-myosin VIIA (Myo7a, cat. no. 3402, Cell Signaling Technology, Inc.), anti-TFAM (cat. no. 8076, Cell Signaling Technology, Inc.), and anti-gasdermin D (GSDMD, Thermo Fisher Scientific, Inc.). Primary

(12) Screening of patient-derived organoids identifies mitophagy as a cell-intrinsic vulnerability in colorectal cancer during statin treatmentCell Reports MedicineApril 15, 2025Zhi-Hang Tao, Ji-Xuan Han, Jia Xu, Enhao Zhao, Ming Wang, Zheng Wang, Xiao-Lin Lin, Xiu-Ying Xiao, Jie Hong, Haoyan Chen, et al.9664), Cleaved PARP (CST, 5625), PINK1 (CST, 6946), p-ULK1 (CST, 5869), LC3B (CST, 3868), TFAM (CST, 8076), and PGC-1α (CST, 2178). The next day, membranes were incubated with HRP-conjugated secondary antibodies

(13) Extracellular Vesicles Released From Skeletal Muscle Post-Chronic Contractile Activity Increase Mitochondrial Biogenesis in Recipient MyoblastsJ Extracell VesiclesApril 10, 2025Patience O. Obi, Tamiris F. G. Souza, Berkay Özerkliğ, Samira Seif, Benjamin Bydak, Nicholas Klassen, Todd A. Duhamel, Adrian R. West, Joseph W. Gordon, Ayesha Saleemoxidative phosphorylation [OXPHOS] cocktail (45‐8099, Invitrogen, 1:500) and rabbit monoclonal anti‐TFAM (8076, Cell Signaling, 1:200). Subsequently, membranes were washed three times for 5 min with TBST, followed

(14) ZNFX1 Functions as a Master Regulator of Epigenetically Induced Pathogen Mimicry and Inflammasome Signaling in CancerCancer ResearchApril 3, 2025Lora Stojanovic, Rachel Abbotts, Kaushelendra Tripathi, Collin M. Coon, Saranya Rajendran, Elnaz Abbasi Abbasi Farid, Galen Hostetter, Joseph W. Guarnieri, Douglas C. Wallace, Sheng Liu, et al.(1:1000, Abcam, #ab179452), anti-MAVS (1:1000, Abcam, #ab290729), anti-TFAM (1:1000, Cell Signaling, #8076), anti-VDAC (1:1000, Cell Signaling, #4866)., STAT3 (1:1000, Cell Signaling, #4904), NFKB 105/50 (1:1000,

(15) Bioengineering the metabolic network of CAR T cells with GLP-1 and Urolithin A increases persistence and long-term anti-tumor activityCell Reports MedicineMarch 18, 2025Areej Akhtar, Md Shakir, Mohammad Sufyan Ansari, Divya, Md Imam Faizan, Varnit Chauhan, Aashi Singh, Ruquaiya Alam, Iqbal Azmi, Sheetal Sharma, et al.antibody,CST,Cat#2118;RRID: AB_561053 mTOR antibody,CST,Cat#2972;RRID: AB_330978 Anti-TFAM antibody,CST,Cat#8076;RRID: AB_10949110 TOM20 antibody,Abcam,Cat#ab56783;RRID: AB_945896 Anti-p62 antibody,Cell Signaling Technology

(16) PGC-1α activation to enhance macrophage immune function in mycobacterial infectionsPLoS OneFebruary 6, 2025Joel R. Frandsen, Zhihong Yuan, Brahmchetna Bedi, Zohra Prasla, Seoung-Ryoung Choi, Prabagaran Narayanasamy, Ruxana T. Sadikot(CST), Danvers, MA 2178), PGC-1α (Santa Cruz Biotechnology (SCBT), Dallas, TX sc-518025), TFAM (CST, 8076), mtTFA (SCBT, sc-166965), β-Actin (CST, 3700), β-Tubulin (CST, 2146) Anti-rabbit IgG HRP (CST, 7074),

(17) Mechanism study on the attenuation of cerebral ischemia-reperfusion injury by LBP extract through regulation of SIRT1/PGC-1α axisTransl NeurosciJanuary 1, 2025Qingfeng Niu, Jiahui Peng, Yujia Zhou, Xiaowen Li, Ouya Liu, Cheng Xin, Ping Liu, Changchun Hei, Xiao YangSC2081-10mM) from Beyotime; and antibodies for SIRT1 (2028S), PGC-1α (2178S), NRF1 (46743S), TFAM (8076S), Bax (2772S), Caspase-3 (9662S), Bcl-2 (3498S), β-actin (4967S), and H3 (9715S) were sourced from Cell Signaling Technology

(18) TR-107, an Agonist of Caseinolytic Peptidase Proteolytic Subunit, Disrupts Mitochondrial Metabolism and Inhibits the Growth of Human Colorectal Cancer CellsMolecular Cancer TherapeuticsDecember 3, 2024Michael Giarrizzo, Joseph F. LaComb, Hetvi R. Patel, Rohan G. Reddy, John D. Haley, Lee M. Graves, Edwin J. Iwanowicz, Agnieszka B. Bialkowska1:1000), anti-TUFM (ThermoFisher, PA5–27511, RRID: AB_2544987, 1:1000), anti-TFAM/mtTFA (Cell Signaling, 8076, RRID: AB_10949110, 1:1000), anti-GPX4 (Cell Signaling, 52455, RRID: AB_2924984, 1:1000), anti-FTH1

(19) CARM1 phosphorylation at S595 by p38γ MAPK drives ROS-mediated cellular senescenceRedox biologyOctober 1, 2024Cho Y, Kim YK.(Santa Cruz Biotechnology, sc-8007), PGC-1α (Santa Cruz Biotechnology, sc-518025), TFAM (Cell Signaling Technology, #8076; Boster Bio, PB9447), TOM20 (ProteinTech, 11802-1-AP; Abnova, H00009804-M01), VDAC1 (Santa Cruz Biotechnology,

(20) Tetramethylpyrazine nitrone exerts neuroprotection via activation of PGC-1α/Nrf2 pathway in Parkinson's disease modelsJournal of advanced researchOctober 1, 2024Guo B, Zheng C, Cao J, Luo F, Li H, Hu S, Mingyuan Lee S, Yang X, Zhang G, Zhang Z, et al.1:50",PA5-38022,Invitrogen PGC-1α,Rabbit polyclonal,IgG,"IF, 1:250",HPA063136,Atlas TFAM,Rabbit monoclonal,IgG,"WB, 1:1000",8076S,CST HO-1,Rabbit monoclonal,IgG,"WB, 1:1000",43966S,CST 8-OHdG,Rabbit polyclonal,IgG,"IF, 1:250",orb16710,Biorbyt 4-HNE,Mouse

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(1)

3/29/2023Highly Efficient Human TFAM Antibody Hyunho LeeThe antibody sensitivity for Western blot analysis is exceptionally high in human cells, including mesenchymal stem cells, HUVECs, and some cancer cell lines. To detect the presence of TFAM protein, ... Read Review
(1)

6/14/2022Good TFAM Antibody for Western Blot Daniel HamiltonCells were seeded and left overnight at 37 °C and 5% CO2. The day after were stimulated for 24 hours with 0.5% FBS. I harvested with lysis buffer and protease inhibitor cocktail 1:100 (v/v). The ... Read Review

TFAM (D5C8) Rabbit mAb

TFAM (D5C8) Rabbit mAb from Cell Signaling Technology

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TFAM (D5C8) Rabbit mAb from Cell Signaling Technology

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