Great Loading Control Antibody for Western Blot

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Techonology

Product Name:

GAPDH (14C10) Rabbit mAb (HRP Conjugate)

Catalog Number:

3683

Clone Number:

N/A

Info:Supplier Page View Product Specs

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The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system – the mammalian defense to challenges against homeostasis. Ketogenic diets (KDs) are extremely high in fats and very low in carbohydrates (calories 90% from fat, 10% from protein, and less than 0.1% from carbohydrates) that rewire whole body metabolism. This metabolic adaptation improves many physiological processes including aging, thermogenesis, and glucose homeostasis. However, the effects of KDs on the immune system remain less explored. Thus, the overall objective of my PhD studies will be to elucidate how diets, particularly KD, affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse liver tissue

Primary Incubation

1:1000

Blocking Agent

5% non fat milk in TBST buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

HRP

Results Summary

10 ug of protein extract was loaded to each lane. As expected, each lane showed similiar level of loading control GAPDH.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great signal.

The Bad

NA

The Bottom Line

A great loading control for a large variety of cell types.

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