GAPDH (14C10) Rabbit mAb (HRP Conjugate) 3683S from Cell Signaling Technology

Product Specs
ItemGAPDH (14C10) Rabbit mAb (HRP Conjugate)
CompanyCell Signaling Technology
Price $450.00 Inquire
Catalog Number3683S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk, B, Pg
TargetGAPDH
HostRabbit
NCBI Gene AliasesG3PD, GAPD, HEL-S-162eP
Conjugate/TagHRP
ClonalityMonoclonal
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase
Research AreaMetabolism
IsotypeIgG
Clone14C10
Molecular Weight37

Questions and Answers

Can this GAPDH antibody be used for Western Blot analysis in various species?

Yes, the GAPDH (14C10) Rabbit mAb (HRP Conjugate) has been shown to work effectively in Western Blot analysis for multiple species, including humans, mice, rats, bovines, and non-human primates, as indicated by its extensive usage in the scientific literature.

Is this antibody suitable for detecting GAPDH in cancer research?

The GAPDH antibody has been utilized in studies related to cancer, including its role in colorectal cancer as demonstrated in research that explores the Hippo signaling pathway and its implications in tumor growth and stem cell properties ().

How does this antibody perform in studies involving neuronal cells?

The GAPDH antibody has been referenced in studies involving neuronal cells, such as in the context of Parkinson's disease research, where it was used to assess protein levels in PC12 cells, indicating its utility in neurobiology ().

What is the significance of using GAPDH as a loading control in Western Blot experiments?

GAPDH is commonly used as a loading control in Western Blot experiments due to its stable expression across various cell types and conditions. This antibody has been cited in numerous studies, including those examining lipid droplet biogenesis and autophagy in cancer cells, reinforcing its reliability as a normalization standard ().

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Valosin-Containing Protein Contributes to Plexiform Neurofibroma Formation and Represents a Novel Therapeutic TargetCellsMay 6, 2026Lalitha Gopalan, Youjin Na, Liang Hu, Ashley Hall, Mi-Ok Kim, Eva Dombi, Sara Szabo, Nancy Ratner, Gang Huang, Jianqiang Wu#58395, 1:1000), CHOP (Cell Signaling Technology; #5554, 1:1000), GAPDH (Cell Signaling Technology; #3683, 1:3000), or β-actin (Cell Signaling Technology; #5125, 1:5000). Antibody binding to the membrane was

(2) QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp–overexpressing cancer modelsMol Cancer TherApril 22, 2026Yang Xie, Ruida Hou, Najah Albadari, Hao Chen, Darcie J. Miller, Judith Quadrozzi Gruntz, Michael L. Oldham, Mir Shahriar Kamal, Jianxiong Jiang, Zhongzhi Wu, et al.were probed overnight at 4 °C with β-tubulin or GAPDH-HRP antibodies (CST #2128, RRID: AB_823664; #3683, RRID: AB_1642205), then with HRP-conjugated secondary antibody (#7074) for 1.5 h at room temperature.

(3) QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp-overexpressing cancer modelsMol Cancer TherApril 22, 2026Yang Xie, Ruida Hou, Najah Albadari, Hao Chen, Darcie J. Miller, Judith Quadrozzi Quadrozzi Gruntz, Michael L. Oldham, Mir Shahriar Kamal, Jianxiong Jiang, Zhongzhi Wu, et al.were probed overnight at 4 °C with β-tubulin or GAPDH-HRP antibodies (CST #2128, RRID: AB_823664; #3683, RRID: AB_1642205), then with HRP-conjugated secondary antibody (#7074) for 1.5 h at room temperature.

(4) Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumorsCell Reports MedicineApril 21, 2026Yizhi Cao, Caleb Cheng, Yitong Yin, Sarah N. Yee, Yang Zheng, Somnath Mahapatra, Radha Paturu, Andrej Coleski, Shannon VanAken, Fan Yang, et al.,Cell Signaling Technology,Cat# 9234; RRID: AB_2269803 GAPDH (14C10) Rabbit mAb (HRP Conjugate),Cell Signaling Technology,Cat# 3683; RRID:AB_1642205 Anti-Ferritin Light Chain antibody,Abcam,Cat# ab69090; RRID: AB_1523609 FTH1 Antibody,Cell Signaling Technology

(5) STAG2 loss amplifies EWS-FLI1-driven microsatellite enhancer activity promoting Ewing sarcoma aggressivenessProc Natl Acad Sci U S AApril 14, 2026Sanjana Eyunni, Shih-Chun Chu, Mary L. Guan, Michaela Louw, Eleanor Young, Sandra E. Carson, Jianhui Gong, Marcin Cieslik, Arul M. Chinnaiyan, Abhijit ParoliaEWS-Fli1 (Abcam:ab15289);H3 (Cell Signaling Technologies: 3638S); GAPDH (Cell Signaling Technologies: 3683); STAG1 (Abcam:ab4457); H3K4me1 (Abcam:ab8895); H3K27ac (Abcam:ab4729). Immunoblotting. Cell lysates

(6) A multi-omics approach to identify the impact of miR-411ed on NSCLC TKI resistancebioRxivApril 3, 2026Daniel Del Valle Morales, Giulia Romano, Michela Saviana, Patrick Nana-Sinkam, Giovanni Nigita, Mario Acunzo(Invitrogen #700139), MET (Cell Signaling #4560), STAT3 (Cell Signaling #7907), and GAPDH (Cell Signaling #3683). RNAseq Sequencing libraries were prepared from the extracted RNA using the Illumina Stranded mRNA

(7) Novel Triazoloquinoxaline-Based Tubulin Polymerization Inhibitor Induces Necroptosis and Significantly Inhibits Metastatic Melanoma Tumor GrowthJournal of Medicinal ChemistryMarch 26, 2026Christopher J. Clark, Shelby N. Waddell, Garrett E. Tessmer, Carl B. Womack, Yang Xie, Brittney D. Williams, Meirola A. Endraws, Cole T. Huddleston, Keiluhn D. Pulis, Joshua Thammathong, et al.Cell Signaling Technology #2128, 1:1000; rabbit anti-GAPDH (HRP Conjugate), Cell Signaling Technology #3683, 1:1000). Proteins were detecting using SuperSignal West Pico PLUS Chemiluminescent Substrate (#34580,

(8) Developmental reprogramming underlies chemotherapy resistance in favorable-histology Wilms tumorCell ReportsMarch 24, 2026Andrew M. Fleming, Carolyn M. Jablonowski, Hongjian Jin, Siwei Zhang, Surbhi Sona, Ha Won Lee, Karissa M. Dieseldorff Jones, Changde Cheng, Beisi Xu, Christopher L. Morton, et al.RRID:AB_2864310" GAPDH (Western Blot; monoclonal; HRP- conjugate; 1:4000 dilution),Cell Signaling Technology,"cat# 3683, RRID:AB_1642205" "Pan-cytokeratin (Western blot, monoclonal mouse; 1:1000 dilution)",Abcam,"Abcam

(9) Deciphering the STAT3-PXN positive feedback loop in GBM, IDH-wildtype: transcriptional regulation and inhibition of YB-1 ubiquitinationCell Death DiscoveryMarch 23, 2026Xiaodong Li, Hongyan Guo, Ziyi Liu, Tianze Wang, Maode Wang, Wei Chen, Hai Yupurchased from Santa Cruz (cat. no. sc-8017).Anti-GAPDH primary antibody was purchased from CST (cat. no. #3683).Anti-beta Actin antibody was purchased from abcam (cat. no. ab115777).Anti-Rabbit-IgG (cat. no. #7074)

(10) TGFβ determines epithelial tissue spacing by regulating mesenchymal condensationbioRxivMarch 20, 2026Chan Jin Park, Pengfei Zhang, Carolina Trenado-Yuste, Celeste M. NelsonThermo Fisher Scientific, 44-244G), pERK1/2 (1:1000; Cell Signaling, 4370), or GAPDH (1:1000; Cell Signaling, 3683) for 2 hours at room temperature. Bands were detected using horseradish-peroxidase-conjugated secondary

(11) Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecovirusesScientific ReportsMarch 18, 2026John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko Iwasakiin this study are commercially available. Anti-MYC-HRP (71D10, #14038) and anti-GAPDH-HRP (14C10, #3683) were purchased from Cell Signaling Technology. Western blot Whole cell lysates from cultured 293T cells

(12) ADAMTS7 promotes smooth muscle foam cell expansion in atherosclerosisJournal of Clinical InvestigationMarch 16, 2026Allen Chung, Lauren E. Fries, Hyun-Kyung Chang, Huize Pan, Alexander C. Bashore, Karissa Shuck, Caio V. Matias, Juliana Gomez Pardo, Jordan S. Kesner, Hanying Yan, et al.(Cell Signaling Technology, 37879S), GAPDH (14C10) rabbit mAb, HRP-conjugated (Cell Signaling Technology, 3683S), anti-COMP (Abcam, ab42225), and rabbit Ab ADAMTS7 (custom-made by 21st Century Biochemicals). The

(13) Heterobifunctional proteomimetic polymers for targeted degradation of MYC and KRASNature CommunicationsFebruary 24, 2026Max M. Wang, Mihai I. Truica, Brayley S. Gattis, Julia Oktawiec, Vinay Sagar, Ananya A. Basu, Paul A. Bertin, Xiaoyu Zhang, Sarki A. Abdulkadir, Nathan C. Gianneschi(Abcam #ab32072), Streptavidin-HRP (PerkinElmer NEL750), KRAS (Abcam # ab275876), GAPDH (Cell Signaling #3683), and Actin (Cell Signaling #5125). Quantification analyses were performed by the Biorad ChemiDoc Imager

(14) Nitric oxide induces p53-mediated cell death in human nasal epithelial cellsScientific ReportsFebruary 20, 2026Shizuki Kamiuezono, Sho Kubota, Tomoki Tsuchida, Nobumasa Takasugi, Takashi Uehara(Cell Signaling Technology, #9542S), Cleaved-PARP (Cell Signaling Technology, #5625S), GAPDH (Cell Signaling Technology, #3683S), anti-rabbit IgG horseradish peroxidase (HRP)-linked F(ab′)2 fragment from donkey (GE Healthcare, Chicago,

(15) The serotonin receptor 2b (5-HT2B) modulates heart remodeling following myocardial infarction via regulation of Hippo pathwayiScienceFebruary 20, 2026Ryan Potter, Min Zi, Sukhpal Prehar, Tara McNulty, Dowan Kwon, Ellie England, Maram Almutairi, Nicholas Stafford, Ardiansah Bayu Nugroho, Efta Triastuti, et al.RRID:AB_10691830 MYPT1,Cell Signalling Technologies,Cat #2634; RRID:AB_915965 GAPDH-HRP,Cell Signalling Technologies,Cat #3683; RRID:AB_1642205 Vinculin,Abcam,Cat #ab91459; RRID:AB_2050446 Mouse anti-α-actinin,Sigma Aldrich,Cat

(16) Lipid-nanoparticle-mediated base editing of the trabecular meshwork rescues glaucoma in vivoJCI InsightFebruary 9, 2026Balasankara Reddy Kaipa, Linya Li, Prakadeeswari Gopalakrishnan, Samuel Du, Jiin Felgner, Krzysztof Palczewski, Philip Felgner, Gulab S. Zodeutilized were against KDEL (catalog ab12223, Abcam), RBPMS (catalog 118619, Gene Tex), GAPDH (catalog 3683, Cell Signaling Technology), TUJ1 (catalog GTX130245, Genetex), Cas9 (catalog 84430, BioLegend), GFAP

(17) CMTM6-Silencing Microbial Immunotherapy Reprograms PDAC Tumors and Restores T-cell FunctionbioRxivFebruary 1, 2026C Y Chabu, R Kazmierczak, M Hasani, N Patterson, Q Wang, L Canti, M.Z Tesfay, A Cios, B Dhagat, M.Q Pastor, et al.1:500 dilution) and HRP-conjugated anti-GAPDH (Cell Signaling Technology, Danvers, MA, USA; catalog# 3683S; 1:1000 dilution). Membranes were developed using SuperSignal West Pico PLUS (Thermo Fisher Scientific;

(18) Cardiomyocyte β-arrestins mediate inflammation and cGAS-STING activation in CVB3 viral myocarditisAmerican Journal of Physiology-Heart and Circulatory PhysiologyFebruary 1, 2026Emilio Y. Lucero, Haoran Jiang, Vincent D’Anniballe, Natalia Pakharukova, Daniella Galtes, Dana Bassford, Arun Jyothidasan, Kirk U. Knowlton, Michael Dee Dee Gunn, Robert J. Lefkowitz, et al.(1:5000) (73671, Cell Signaling, RRID: AB_2799843), monoclonal HRP conjugated GADPH antibody (1:1000) (3683, Cell Signaling, RRID:AB_1642205). Secondary antibodies included HRP conjugated anti-rabbit (NA934,

(19) Development of a quadruple-conjugated carbon dot nanomodel for targeted glioma therapyCommunications ChemistryJanuary 30, 2026Emel Kirbas Cilingir, Sajini D. Hettiarachchi, Parth Rathee, Yiqun Zhou, Braulio CLB Ferreira, Lukun Wang, Annu Joji, Carlos M. Gonzalez, Maria J. Moreno Hollweg, Mehrdad Shiri, et al.purchased from Thermo Scientific (Waltham, MA, USA), and GAPDH ((14C10) Rabbit Monoclonal Antibody, #3683) was purchased from Cell Signaling Technology (Danvers, MA, USA). These antibodies were previously validated

(20) ADAMTS7 promotes smooth muscle foam cell expansion in atherosclerosisJ Clin InvestJanuary 29, 2026Allen Chung, Lauren E. Fries, Hyun-Kyung Chang, Huize Pan, Alexander C. Bashore, Karissa Shuck, Caio V. Matias, Juliana Gomez Pardo, Jordan S. Kesner, Hanying Yan, et al.(Cell Signaling Technology, 37879S), GAPDH (14C10) rabbit mAb, HRP-conjugated (Cell Signaling Technology, 3683S), anti-COMP (Abcam, ab42225), and rabbit Ab ADAMTS7 (custom-made by 21st Century Biochemicals). The

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5/23/2022Great Loading Control Antibody for Western Blot Owen JiangThe nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system – the mammalian defense to challenges against ... Read Review

GAPDH (14C10) Rabbit mAb (HRP Conjugate)

GAPDH (14C10) Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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GAPDH (14C10) Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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