Beckman Coulter
CD27-PB, 1A4CD27, 0.5 mL, ASR
B30635
1A4CD27
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Our assay focuses on the detection of CD27 on normal cells and how it changes in malignancy. We were also interested in testing CD27 on plasma cells as a marker for plasma cell dyscrasia and multiple myeloma.
Flow Cytometry
Peripheral blood (human)
10-15 minutes @ Room Temp
1% FBS
N/A
Flow cytometer equipped with violet laser
As shown in the dot-plot, the antibody produced reasonable, albeit dim, staining on the lymphocyte population (in red). Presumably, these are T cells. However, the dim signal means that it's very hard to distinguish dim populations, and hence difficult to appreciate the subtle changes seen in leukemia and lymphoma.
Easy to optimize. ASR.
Expensive. Dim signal.
This antibody may work for many common applications, and labs should certainly try it when they need CD27 in a violet-laser channel.