Time Course Effect of Chemoattractant on SNAIL Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling

Product Name:

Snail Antibody

Catalog Number:

3879

Clone Number:

C15D3

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Zinc finger protein SNAI1 is a protein that in humans is encoded by the SNAI1 gene. Here we checked the time course effect of SNAIL by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Snail is a family of transcription factors that promote the repression of the adhesion molecule E-cadherin to regulate epithelial to mesenchymal transition during embryonic development. We found increase in Snail expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Antibody works great for western blot.

The Bad

None

The Bottom Line

Works great for western blot and immunoprecipitation but not for EMSA. Highly recommended.

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