Efficient DC Antibody from BioLegend

Chemical/Biomedical Engineering
University of Toronto
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

aCD11c BV750

Catalog Number:

117357

Clone Number:

N418

Info:Supplier Page View Product Specs

Browse Similar Products: CD11c Antibodies

We analyze mechanisms responsible for immune tolerance using single cell analysis methods such as flow cytometry, single cell omics, etc.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus and lymph nodes

Primary Incubation

Block: 1:50 in 100uL at 37C for 30 min

Blocking Agent

Fc Block (aCD16/32)

Secondary Incubation

L/D: Zombie NIR 1:4000 in 100 uL at RT for 20 min

Tertiary Incubation

Surface stain: 1:400 in 100uL at 4C for 30 min

Detection

Spectral flow cytometry (Cytek Aurora)

Results Summary

This marker works reasonably well for identifying CD11c+ dendritic cells when used alongside other DC markers, but is not a clear enough signal on its own.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Affordable, reasonable quality when used in a DC panel.

The Bad

Not a clear marker by itself.

The Bottom Line

Good for use in studying DC subsets if other antibodies are being used anyways.

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