Chemoattractant on Rab27A Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Invitrogen

Product Name:

RAB27A Polyclonal Antibody

Catalog Number:

PA5-47907

Clone Number:

N/A

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Here we checked the time course effect of Rab27A by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of Rab 27A expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Highly specific and strong signal

The Bad

None

The Bottom Line

This antibody works great for western blot, immunoprecipitation and immunostaining studies. Highly recommended.

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